Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0162 and BT0163 are separated by 45 nucleotides BT0163 and BT0164 are separated by 7 nucleotides
BT0162: BT0162 - penicillin-binding protein 1C (PBP-1c) (NCBI ptt file), at 167,345 to 169,663
BT0162
BT0163: BT0163 - conserved hypothetical protein (NCBI ptt file), at 169,709 to 169,975
BT0163
BT0164: BT0164 - conserved hypothetical protein (NCBI ptt file), at 169,983 to 175,358
BT0164
Position (kb) 169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 168.810 kb on - strand, within BT0162 at 168.810 kb on - strand, within BT0162 at 169.018 kb on - strand, within BT0162 at 169.182 kb on + strand, within BT0162 at 169.183 kb on - strand, within BT0162 at 169.183 kb on - strand, within BT0162 at 169.310 kb on - strand, within BT0162 at 169.323 kb on - strand, within BT0162 at 169.418 kb on - strand at 169.418 kb on - strand, within BT0162 at 169.573 kb on + strand at 169.576 kb on - strand at 169.607 kb on + strand at 169.669 kb on + strand at 169.670 kb on - strand at 169.675 kb on - strand at 169.693 kb on - strand at 169.768 kb on + strand, within BT0163 at 169.796 kb on - strand, within BT0163 at 169.867 kb on - strand, within BT0163 at 169.898 kb on - strand, within BT0163 at 169.903 kb on + strand, within BT0163 at 169.905 kb on + strand, within BT0163 at 169.986 kb on + strand at 169.986 kb on + strand at 169.988 kb on + strand at 170.006 kb on + strand at 170.053 kb on + strand at 170.130 kb on - strand at 170.130 kb on - strand at 170.180 kb on + strand at 170.180 kb on + strand at 170.563 kb on + strand, within BT0164 at 170.734 kb on + strand, within BT0164 at 170.770 kb on - strand, within BT0164 at 170.770 kb on - strand, within BT0164 at 170.798 kb on + strand, within BT0164 at 170.798 kb on + strand, within BT0164 at 170.906 kb on - strand, within BT0164
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 168,810 - BT0162 0.63 -1.5 168,810 - BT0162 0.63 -1.0 169,018 - BT0162 0.72 -1.5 169,182 + BT0162 0.79 -1.2 169,183 - BT0162 0.79 -2.0 169,183 - BT0162 0.79 -0.5 169,310 - BT0162 0.85 -3.1 169,323 - BT0162 0.85 -1.2 169,418 - +0.5 169,418 - BT0162 0.89 -0.7 169,573 + -2.3 169,576 - -0.9 169,607 + -1.5 169,669 + -0.9 169,670 - -1.2 169,675 - +0.5 169,693 - -0.5 169,768 + BT0163 0.22 -0.5 169,796 - BT0163 0.33 -2.3 169,867 - BT0163 0.59 -1.7 169,898 - BT0163 0.71 -3.0 169,903 + BT0163 0.73 +2.1 169,905 + BT0163 0.73 -0.5 169,986 + +0.1 169,986 + -0.9 169,988 + -0.4 170,006 + +0.2 170,053 + +0.1 170,130 - -0.5 170,130 - -1.9 170,180 + -2.9 170,180 + -1.2 170,563 + BT0164 0.11 -1.5 170,734 + BT0164 0.14 +1.7 170,770 - BT0164 0.15 -0.5 170,770 - BT0164 0.15 -2.2 170,798 + BT0164 0.15 -0.7 170,798 + BT0164 0.15 +0.6 170,906 - BT0164 0.17 +0.1
Or see this region's nucleotide sequence