Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5815 and Pf1N1B4_5816 are separated by 150 nucleotides Pf1N1B4_5816 and Pf1N1B4_5817 are separated by 227 nucleotides Pf1N1B4_5817 and Pf1N1B4_5818 are separated by 192 nucleotides
Pf1N1B4_5815 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 247,219 to 248,796
_5815
Pf1N1B4_5816 - FIG00957304: hypothetical protein, at 248,947 to 249,474
_5816
Pf1N1B4_5817 - Decarboxylase family protein, at 249,702 to 251,075
_5817
Pf1N1B4_5818 - hypothetical protein, at 251,268 to 251,627
_5818
Position (kb) 249
250
251
252 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 248.815 kb on + strand at 248.883 kb on - strand at 248.900 kb on - strand at 248.949 kb on - strand at 249.376 kb on + strand at 249.377 kb on - strand, within Pf1N1B4_5816 at 249.441 kb on - strand at 249.502 kb on - strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.556 kb on - strand at 249.556 kb on - strand at 249.560 kb on - strand at 249.560 kb on - strand at 249.560 kb on - strand at 249.611 kb on - strand at 249.642 kb on + strand at 249.649 kb on + strand at 249.718 kb on + strand at 249.718 kb on + strand at 249.718 kb on + strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.741 kb on - strand at 249.776 kb on - strand at 249.810 kb on + strand at 249.811 kb on - strand at 249.892 kb on - strand, within Pf1N1B4_5817 at 250.203 kb on + strand, within Pf1N1B4_5817 at 250.204 kb on - strand, within Pf1N1B4_5817 at 250.204 kb on - strand at 250.204 kb on - strand, within Pf1N1B4_5817 at 250.507 kb on - strand at 250.650 kb on + strand, within Pf1N1B4_5817 at 250.651 kb on - strand, within Pf1N1B4_5817 at 251.046 kb on + strand at 251.046 kb on + strand at 251.047 kb on - strand at 251.094 kb on + strand at 251.157 kb on - strand at 251.157 kb on - strand at 251.164 kb on - strand at 251.525 kb on - strand, within Pf1N1B4_5818 at 251.525 kb on - strand, within Pf1N1B4_5818 at 251.639 kb on + strand at 251.690 kb on + strand at 251.690 kb on + strand at 251.692 kb on + strand at 251.692 kb on + strand at 251.693 kb on - strand at 251.693 kb on - strand at 251.716 kb on + strand at 251.717 kb on - strand at 251.746 kb on + strand at 251.757 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.776 kb on + strand at 251.777 kb on - strand at 251.777 kb on - strand at 251.777 kb on - strand at 251.777 kb on - strand at 251.814 kb on + strand at 251.947 kb on + strand at 251.947 kb on + strand at 251.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 248,815 + -1.0 248,883 - -0.3 248,900 - +0.6 248,949 - +0.7 249,376 + +0.3 249,377 - Pf1N1B4_5816 0.81 -2.7 249,441 - -1.7 249,502 - +0.9 249,555 + -2.1 249,555 + +0.5 249,555 + +0.4 249,555 + -0.6 249,555 + -1.2 249,555 + +2.2 249,556 - +0.2 249,556 - +0.6 249,560 - +0.3 249,560 - -0.1 249,560 - -1.4 249,611 - +0.6 249,642 + +0.4 249,649 + -1.4 249,718 + +0.1 249,718 + +0.6 249,718 + -0.1 249,719 - -0.1 249,719 - -0.3 249,719 - -2.1 249,719 - -0.7 249,719 - +0.1 249,741 - +1.3 249,776 - +0.6 249,810 + +0.4 249,811 - +1.6 249,892 - Pf1N1B4_5817 0.14 +0.1 250,203 + Pf1N1B4_5817 0.36 -1.7 250,204 - Pf1N1B4_5817 0.37 +0.3 250,204 - +0.6 250,204 - Pf1N1B4_5817 0.37 -2.7 250,507 - -2.6 250,650 + Pf1N1B4_5817 0.69 -3.3 250,651 - Pf1N1B4_5817 0.69 -2.5 251,046 + -1.9 251,046 + +1.6 251,047 - +1.3 251,094 + -0.4 251,157 - -0.0 251,157 - +0.0 251,164 - -0.2 251,525 - Pf1N1B4_5818 0.71 +0.5 251,525 - Pf1N1B4_5818 0.71 -0.6 251,639 + -0.6 251,690 + +0.4 251,690 + -0.1 251,692 + +0.7 251,692 + +0.4 251,693 - +0.6 251,693 - +0.4 251,716 + +0.9 251,717 - -0.5 251,746 + -1.1 251,757 + -0.1 251,776 + -0.2 251,776 + +0.1 251,776 + +1.7 251,776 + -0.4 251,776 + -1.7 251,776 + +0.5 251,776 + -0.4 251,776 + -0.2 251,776 + -0.0 251,777 - -2.2 251,777 - +2.2 251,777 - -0.9 251,777 - -2.2 251,814 + +0.4 251,947 + -0.3 251,947 + -2.2 251,948 - -0.5
Or see this region's nucleotide sequence