Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4028 and Pf1N1B4_4029 are separated by 7 nucleotides Pf1N1B4_4029 and Pf1N1B4_4030 are separated by 39 nucleotides
Pf1N1B4_4028 - NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3), at 4,478,252 to 4,479,715
_4028
Pf1N1B4_4029 - NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3), at 4,479,723 to 4,481,255
_4029
Pf1N1B4_4030 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 4,481,295 to 4,483,148
_4030
Position (kb)
4479
4480
4481
4482 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4479.228 kb on + strand, within Pf1N1B4_4028 at 4479.228 kb on + strand, within Pf1N1B4_4028 at 4479.229 kb on - strand, within Pf1N1B4_4028 at 4479.435 kb on + strand, within Pf1N1B4_4028 at 4479.489 kb on + strand, within Pf1N1B4_4028 at 4479.490 kb on - strand, within Pf1N1B4_4028 at 4479.830 kb on - strand at 4480.155 kb on - strand, within Pf1N1B4_4029 at 4480.162 kb on + strand, within Pf1N1B4_4029 at 4480.259 kb on - strand, within Pf1N1B4_4029 at 4480.259 kb on - strand, within Pf1N1B4_4029 at 4480.352 kb on - strand, within Pf1N1B4_4029 at 4480.444 kb on + strand, within Pf1N1B4_4029 at 4480.444 kb on + strand, within Pf1N1B4_4029 at 4480.588 kb on + strand, within Pf1N1B4_4029 at 4480.801 kb on + strand, within Pf1N1B4_4029 at 4480.802 kb on - strand, within Pf1N1B4_4029 at 4481.325 kb on + strand at 4481.861 kb on - strand, within Pf1N1B4_4030 at 4481.861 kb on - strand, within Pf1N1B4_4030 at 4481.954 kb on - strand, within Pf1N1B4_4030 at 4482.168 kb on + strand, within Pf1N1B4_4030 at 4482.215 kb on - strand, within Pf1N1B4_4030
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 4,479,228 + Pf1N1B4_4028 0.67 -1.6 4,479,228 + Pf1N1B4_4028 0.67 -2.0 4,479,229 - Pf1N1B4_4028 0.67 +0.0 4,479,435 + Pf1N1B4_4028 0.81 -1.7 4,479,489 + Pf1N1B4_4028 0.84 -1.4 4,479,490 - Pf1N1B4_4028 0.85 -2.7 4,479,830 - -1.7 4,480,155 - Pf1N1B4_4029 0.28 -2.2 4,480,162 + Pf1N1B4_4029 0.29 -2.4 4,480,259 - Pf1N1B4_4029 0.35 -3.1 4,480,259 - Pf1N1B4_4029 0.35 -1.8 4,480,352 - Pf1N1B4_4029 0.41 -1.8 4,480,444 + Pf1N1B4_4029 0.47 -1.1 4,480,444 + Pf1N1B4_4029 0.47 -2.2 4,480,588 + Pf1N1B4_4029 0.56 -3.2 4,480,801 + Pf1N1B4_4029 0.70 -2.1 4,480,802 - Pf1N1B4_4029 0.70 -1.8 4,481,325 + -3.5 4,481,861 - Pf1N1B4_4030 0.31 -2.4 4,481,861 - Pf1N1B4_4030 0.31 -2.4 4,481,954 - Pf1N1B4_4030 0.36 -2.6 4,482,168 + Pf1N1B4_4030 0.47 +0.0 4,482,215 - Pf1N1B4_4030 0.50 -0.2
Or see this region's nucleotide sequence