Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4022 and Pf1N1B4_4023 are separated by 79 nucleotides Pf1N1B4_4023 and Pf1N1B4_4024 are separated by 477 nucleotides Pf1N1B4_4024 and Pf1N1B4_4025 are separated by 207 nucleotides Pf1N1B4_4025 and Pf1N1B4_4026 overlap by 4 nucleotides
Pf1N1B4_4022 - hypothetical protein, at 4,474,955 to 4,475,155
_4022
Pf1N1B4_4023 - hypothetical protein, at 4,475,235 to 4,475,567
_4023
Pf1N1B4_4024 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 4,476,045 to 4,476,425
_4024
Pf1N1B4_4025 - Outer membrane porin, OprD family, at 4,476,633 to 4,477,394
_4025
Pf1N1B4_4026 - Outer membrane porin, OprD family, at 4,477,391 to 4,477,894
_4026
Position (kb)
4476
4477 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4475.143 kb on + strand at 4475.153 kb on + strand at 4475.153 kb on + strand at 4475.153 kb on + strand at 4475.153 kb on + strand at 4475.153 kb on + strand at 4475.154 kb on - strand at 4475.234 kb on + strand at 4475.235 kb on - strand at 4475.235 kb on - strand at 4475.340 kb on - strand, within Pf1N1B4_4023 at 4475.361 kb on + strand, within Pf1N1B4_4023 at 4475.480 kb on + strand, within Pf1N1B4_4023 at 4475.493 kb on + strand, within Pf1N1B4_4023 at 4475.493 kb on + strand, within Pf1N1B4_4023 at 4475.493 kb on + strand, within Pf1N1B4_4023 at 4475.493 kb on + strand, within Pf1N1B4_4023 at 4475.516 kb on + strand, within Pf1N1B4_4023 at 4475.516 kb on + strand, within Pf1N1B4_4023 at 4475.516 kb on + strand, within Pf1N1B4_4023 at 4475.530 kb on - strand, within Pf1N1B4_4023 at 4475.530 kb on - strand, within Pf1N1B4_4023 at 4475.693 kb on + strand at 4475.704 kb on + strand at 4475.704 kb on + strand at 4475.705 kb on - strand at 4475.741 kb on + strand at 4475.741 kb on + strand at 4475.742 kb on - strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.875 kb on + strand at 4475.876 kb on - strand at 4475.876 kb on - strand at 4475.876 kb on - strand at 4475.876 kb on - strand at 4475.901 kb on - strand at 4475.978 kb on - strand at 4475.978 kb on - strand at 4476.024 kb on + strand at 4476.165 kb on + strand, within Pf1N1B4_4024 at 4476.166 kb on - strand, within Pf1N1B4_4024 at 4476.168 kb on + strand, within Pf1N1B4_4024 at 4476.168 kb on + strand, within Pf1N1B4_4024 at 4476.168 kb on + strand, within Pf1N1B4_4024 at 4476.169 kb on - strand, within Pf1N1B4_4024 at 4476.169 kb on - strand at 4476.423 kb on + strand at 4476.423 kb on + strand at 4476.423 kb on + strand at 4476.424 kb on - strand at 4476.543 kb on + strand at 4476.544 kb on - strand at 4476.584 kb on - strand at 4476.584 kb on - strand at 4476.606 kb on - strand at 4476.653 kb on - strand at 4476.669 kb on + strand at 4476.763 kb on + strand at 4476.764 kb on - strand, within Pf1N1B4_4025 at 4476.764 kb on - strand, within Pf1N1B4_4025 at 4476.764 kb on - strand, within Pf1N1B4_4025 at 4476.764 kb on - strand, within Pf1N1B4_4025 at 4476.764 kb on - strand, within Pf1N1B4_4025 at 4476.764 kb on - strand, within Pf1N1B4_4025 at 4476.835 kb on + strand, within Pf1N1B4_4025 at 4476.835 kb on + strand, within Pf1N1B4_4025 at 4476.835 kb on + strand at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.836 kb on - strand, within Pf1N1B4_4025 at 4476.857 kb on - strand, within Pf1N1B4_4025 at 4476.913 kb on + strand, within Pf1N1B4_4025 at 4476.913 kb on + strand, within Pf1N1B4_4025 at 4476.913 kb on + strand, within Pf1N1B4_4025 at 4476.914 kb on - strand, within Pf1N1B4_4025 at 4476.979 kb on + strand, within Pf1N1B4_4025 at 4476.980 kb on - strand, within Pf1N1B4_4025 at 4477.162 kb on + strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.166 kb on - strand, within Pf1N1B4_4025 at 4477.166 kb on - strand, within Pf1N1B4_4025 at 4477.166 kb on - strand, within Pf1N1B4_4025 at 4477.166 kb on - strand, within Pf1N1B4_4025 at 4477.228 kb on + strand, within Pf1N1B4_4025 at 4477.228 kb on + strand, within Pf1N1B4_4025 at 4477.229 kb on - strand, within Pf1N1B4_4025 at 4477.229 kb on - strand, within Pf1N1B4_4025 at 4477.229 kb on - strand, within Pf1N1B4_4025 at 4477.232 kb on - strand, within Pf1N1B4_4025 at 4477.232 kb on - strand, within Pf1N1B4_4025 at 4477.232 kb on - strand, within Pf1N1B4_4025 at 4477.265 kb on - strand, within Pf1N1B4_4025 at 4477.298 kb on - strand, within Pf1N1B4_4025 at 4477.345 kb on + strand at 4477.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 4,475,143 + -0.9 4,475,153 + +0.3 4,475,153 + +0.6 4,475,153 + -1.3 4,475,153 + -2.5 4,475,153 + -1.5 4,475,154 - +0.8 4,475,234 + +0.8 4,475,235 - -0.2 4,475,235 - -0.2 4,475,340 - Pf1N1B4_4023 0.32 +1.8 4,475,361 + Pf1N1B4_4023 0.38 -1.7 4,475,480 + Pf1N1B4_4023 0.74 +0.9 4,475,493 + Pf1N1B4_4023 0.77 +0.6 4,475,493 + Pf1N1B4_4023 0.77 -1.5 4,475,493 + Pf1N1B4_4023 0.77 +1.0 4,475,493 + Pf1N1B4_4023 0.77 +1.3 4,475,516 + Pf1N1B4_4023 0.84 -2.3 4,475,516 + Pf1N1B4_4023 0.84 -2.7 4,475,516 + Pf1N1B4_4023 0.84 +0.6 4,475,530 - Pf1N1B4_4023 0.89 +0.0 4,475,530 - Pf1N1B4_4023 0.89 +0.6 4,475,693 + +0.3 4,475,704 + -0.5 4,475,704 + +1.4 4,475,705 - -0.2 4,475,741 + +0.1 4,475,741 + -1.0 4,475,742 - -2.4 4,475,875 + -0.4 4,475,875 + -2.4 4,475,875 + -0.1 4,475,875 + -0.4 4,475,875 + +2.6 4,475,875 + +0.2 4,475,876 - -1.2 4,475,876 - -1.8 4,475,876 - +0.4 4,475,876 - +0.5 4,475,901 - -2.2 4,475,978 - -1.6 4,475,978 - +0.4 4,476,024 + -1.5 4,476,165 + Pf1N1B4_4024 0.31 -1.5 4,476,166 - Pf1N1B4_4024 0.32 +0.6 4,476,168 + Pf1N1B4_4024 0.32 -0.3 4,476,168 + Pf1N1B4_4024 0.32 -1.8 4,476,168 + Pf1N1B4_4024 0.32 -1.2 4,476,169 - Pf1N1B4_4024 0.33 -0.1 4,476,169 - +0.7 4,476,423 + +0.9 4,476,423 + +1.3 4,476,423 + +0.3 4,476,424 - -1.3 4,476,543 + -0.8 4,476,544 - -1.7 4,476,584 - -0.3 4,476,584 - -1.4 4,476,606 - -0.2 4,476,653 - -3.4 4,476,669 + +0.3 4,476,763 + -2.3 4,476,764 - Pf1N1B4_4025 0.17 -0.2 4,476,764 - Pf1N1B4_4025 0.17 +0.0 4,476,764 - Pf1N1B4_4025 0.17 +0.4 4,476,764 - Pf1N1B4_4025 0.17 +0.1 4,476,764 - Pf1N1B4_4025 0.17 -0.2 4,476,764 - Pf1N1B4_4025 0.17 -0.9 4,476,835 + Pf1N1B4_4025 0.27 -1.4 4,476,835 + Pf1N1B4_4025 0.27 -0.5 4,476,835 + -1.2 4,476,836 - Pf1N1B4_4025 0.27 -0.8 4,476,836 - Pf1N1B4_4025 0.27 -0.5 4,476,836 - Pf1N1B4_4025 0.27 +0.6 4,476,836 - Pf1N1B4_4025 0.27 -1.0 4,476,836 - -0.5 4,476,836 - Pf1N1B4_4025 0.27 -0.8 4,476,836 - Pf1N1B4_4025 0.27 -0.8 4,476,836 - Pf1N1B4_4025 0.27 -0.2 4,476,836 - Pf1N1B4_4025 0.27 -1.6 4,476,836 - Pf1N1B4_4025 0.27 -1.2 4,476,836 - Pf1N1B4_4025 0.27 -0.3 4,476,836 - Pf1N1B4_4025 0.27 +0.5 4,476,857 - Pf1N1B4_4025 0.29 -0.0 4,476,913 + Pf1N1B4_4025 0.37 -0.1 4,476,913 + Pf1N1B4_4025 0.37 +1.1 4,476,913 + Pf1N1B4_4025 0.37 +0.7 4,476,914 - Pf1N1B4_4025 0.37 +0.2 4,476,979 + Pf1N1B4_4025 0.45 -0.1 4,476,980 - Pf1N1B4_4025 0.46 -0.6 4,477,162 + Pf1N1B4_4025 0.69 -0.3 4,477,163 - Pf1N1B4_4025 0.70 -0.7 4,477,163 - Pf1N1B4_4025 0.70 -0.7 4,477,163 - Pf1N1B4_4025 0.70 -0.4 4,477,163 - Pf1N1B4_4025 0.70 -0.6 4,477,163 - Pf1N1B4_4025 0.70 -1.4 4,477,163 - Pf1N1B4_4025 0.70 +1.4 4,477,163 - Pf1N1B4_4025 0.70 -0.2 4,477,163 - Pf1N1B4_4025 0.70 +0.9 4,477,163 - Pf1N1B4_4025 0.70 -2.3 4,477,163 - Pf1N1B4_4025 0.70 -2.3 4,477,163 - Pf1N1B4_4025 0.70 +0.2 4,477,166 - Pf1N1B4_4025 0.70 +0.4 4,477,166 - Pf1N1B4_4025 0.70 -1.2 4,477,166 - Pf1N1B4_4025 0.70 -0.3 4,477,166 - Pf1N1B4_4025 0.70 -0.7 4,477,228 + Pf1N1B4_4025 0.78 -0.1 4,477,228 + Pf1N1B4_4025 0.78 +0.0 4,477,229 - Pf1N1B4_4025 0.78 +0.6 4,477,229 - Pf1N1B4_4025 0.78 -0.6 4,477,229 - Pf1N1B4_4025 0.78 +1.4 4,477,232 - Pf1N1B4_4025 0.79 +1.2 4,477,232 - Pf1N1B4_4025 0.79 -0.4 4,477,232 - Pf1N1B4_4025 0.79 -0.1 4,477,265 - Pf1N1B4_4025 0.83 +0.1 4,477,298 - Pf1N1B4_4025 0.87 -0.1 4,477,345 + -1.8 4,477,346 - +1.5
Or see this region's nucleotide sequence