Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_376 and Pf1N1B4_377 are separated by 211 nucleotides Pf1N1B4_377 and Pf1N1B4_378 are separated by 5 nucleotides Pf1N1B4_378 and Pf1N1B4_379 are separated by 153 nucleotides Pf1N1B4_379 and Pf1N1B4_380 are separated by 64 nucleotides
Pf1N1B4_376 - Deblocking aminopeptidase (EC 3.4.11.-) @ Cyanophycinase 2 (EC 3.4.15.6), at 447,403 to 448,593
_376
Pf1N1B4_377 - FIG00953242: hypothetical protein, at 448,805 to 449,032
_377
Pf1N1B4_378 - Carbon storage regulator, at 449,038 to 449,223
_378
Pf1N1B4_379 - Protein of unknown function UPF0060, at 449,377 to 449,709
_379
Pf1N1B4_380 - Oxidoreductase, short chain dehydrogenase/reductase family, at 449,774 to 450,589
_380
Position (kb) 449
450 Strain fitness (log2 ratio) -2
-1
0
1
2 at 448.438 kb on + strand, within Pf1N1B4_376 at 448.438 kb on + strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.747 kb on - strand at 448.762 kb on - strand at 448.804 kb on + strand at 448.804 kb on + strand at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.216 kb on - strand at 449.312 kb on - strand at 449.312 kb on - strand at 449.358 kb on + strand at 449.359 kb on - strand at 449.510 kb on + strand, within Pf1N1B4_379 at 449.510 kb on + strand, within Pf1N1B4_379 at 449.510 kb on + strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.522 kb on + strand, within Pf1N1B4_379 at 449.523 kb on - strand, within Pf1N1B4_379 at 449.625 kb on + strand at 449.625 kb on + strand, within Pf1N1B4_379 at 449.626 kb on - strand, within Pf1N1B4_379 at 449.699 kb on + strand at 449.699 kb on + strand at 449.699 kb on + strand at 449.699 kb on + strand at 449.699 kb on + strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.700 kb on - strand at 449.835 kb on + strand at 449.836 kb on - strand at 449.836 kb on - strand at 449.928 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.935 kb on - strand, within Pf1N1B4_380 at 449.935 kb on - strand, within Pf1N1B4_380 at 449.935 kb on - strand at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.641 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 448,438 + Pf1N1B4_376 0.87 -0.3 448,438 + Pf1N1B4_376 0.87 -0.4 448,439 - Pf1N1B4_376 0.87 +1.0 448,439 - Pf1N1B4_376 0.87 -0.4 448,439 - Pf1N1B4_376 0.87 -1.1 448,439 - Pf1N1B4_376 0.87 -1.8 448,747 - -0.2 448,762 - -0.0 448,804 + +1.4 448,804 + +0.4 449,082 - Pf1N1B4_378 0.24 -1.0 449,082 - Pf1N1B4_378 0.24 +0.6 449,082 - Pf1N1B4_378 0.24 +0.7 449,082 - Pf1N1B4_378 0.24 +1.2 449,098 + Pf1N1B4_378 0.32 +1.4 449,098 + Pf1N1B4_378 0.32 -0.8 449,098 + Pf1N1B4_378 0.32 -0.8 449,099 - Pf1N1B4_378 0.33 -1.3 449,099 - Pf1N1B4_378 0.33 -0.5 449,099 - Pf1N1B4_378 0.33 +0.0 449,099 - Pf1N1B4_378 0.33 +0.7 449,099 - Pf1N1B4_378 0.33 +0.1 449,216 - -1.3 449,312 - -0.1 449,312 - +0.5 449,358 + -0.1 449,359 - -0.2 449,510 + Pf1N1B4_379 0.40 -1.7 449,510 + Pf1N1B4_379 0.40 -0.2 449,510 + Pf1N1B4_379 0.40 +1.1 449,511 - Pf1N1B4_379 0.40 -0.9 449,511 - Pf1N1B4_379 0.40 +1.1 449,511 - Pf1N1B4_379 0.40 +1.0 449,511 - Pf1N1B4_379 0.40 +0.4 449,511 - Pf1N1B4_379 0.40 -0.5 449,511 - Pf1N1B4_379 0.40 -1.1 449,522 + Pf1N1B4_379 0.44 -0.7 449,523 - Pf1N1B4_379 0.44 +0.4 449,625 + +0.5 449,625 + Pf1N1B4_379 0.74 -0.5 449,626 - Pf1N1B4_379 0.75 -0.8 449,699 + +1.3 449,699 + +0.1 449,699 + -1.7 449,699 + +1.0 449,699 + +0.4 449,700 - +0.3 449,700 - -0.9 449,700 - -0.8 449,700 - +0.4 449,700 - -0.3 449,700 - -1.2 449,700 - -0.7 449,700 - -0.3 449,835 + +1.0 449,836 - +1.5 449,836 - -0.5 449,928 + Pf1N1B4_380 0.19 +0.8 449,934 + Pf1N1B4_380 0.20 -0.9 449,934 + Pf1N1B4_380 0.20 -0.2 449,934 + Pf1N1B4_380 0.20 +0.6 449,935 - Pf1N1B4_380 0.20 +0.8 449,935 - Pf1N1B4_380 0.20 +0.3 449,935 - +0.2 450,141 + Pf1N1B4_380 0.45 -1.4 450,141 + Pf1N1B4_380 0.45 +2.2 450,141 + Pf1N1B4_380 0.45 +0.2 450,141 + Pf1N1B4_380 0.45 +0.2 450,142 - Pf1N1B4_380 0.45 +0.7 450,142 - Pf1N1B4_380 0.45 -1.7 450,142 - -1.5 450,142 - Pf1N1B4_380 0.45 +1.3 450,142 - Pf1N1B4_380 0.45 +1.3 450,142 - Pf1N1B4_380 0.45 -0.4 450,641 - -1.2
Or see this region's nucleotide sequence