Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2722

Experiment: nitrogen source nitrite 1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2721 and Pf1N1B4_2722 are separated by 71 nucleotidesPf1N1B4_2722 and Pf1N1B4_2723 overlap by 6 nucleotidesPf1N1B4_2723 and Pf1N1B4_2724 are separated by 187 nucleotides Pf1N1B4_2721 - AMP nucleosidase (EC 3.2.2.4), at 3,059,894 to 3,061,357 _2721 Pf1N1B4_2722 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 3,061,429 to 3,062,064 _2722 Pf1N1B4_2723 - FIG00954142: hypothetical protein, at 3,062,059 to 3,062,268 _2723 Pf1N1B4_2724 - Transcriptional regulator, AsnC family, at 3,062,456 to 3,062,935 _2724 Position (kb) 3061 3062 3063Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3060.464 kb on + strand, within Pf1N1B4_2721at 3060.465 kb on - strand, within Pf1N1B4_2721at 3060.465 kb on - strand, within Pf1N1B4_2721at 3060.465 kb on - strand, within Pf1N1B4_2721at 3060.515 kb on + strand, within Pf1N1B4_2721at 3060.515 kb on + strand, within Pf1N1B4_2721at 3060.515 kb on + strand, within Pf1N1B4_2721at 3060.516 kb on - strand, within Pf1N1B4_2721at 3060.689 kb on + strandat 3060.854 kb on + strand, within Pf1N1B4_2721at 3060.854 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.873 kb on - strand, within Pf1N1B4_2721at 3060.873 kb on - strand, within Pf1N1B4_2721at 3060.873 kb on - strand, within Pf1N1B4_2721at 3060.873 kb on - strand, within Pf1N1B4_2721at 3061.034 kb on + strand, within Pf1N1B4_2721at 3061.034 kb on + strand, within Pf1N1B4_2721at 3061.034 kb on + strand, within Pf1N1B4_2721at 3061.034 kb on + strand, within Pf1N1B4_2721at 3061.035 kb on - strand, within Pf1N1B4_2721at 3061.035 kb on - strand, within Pf1N1B4_2721at 3061.035 kb on - strand, within Pf1N1B4_2721at 3061.235 kb on + strandat 3061.235 kb on + strandat 3061.236 kb on - strandat 3061.236 kb on - strandat 3061.236 kb on - strandat 3061.236 kb on - strandat 3061.236 kb on - strandat 3061.390 kb on + strandat 3061.412 kb on + strandat 3061.413 kb on - strandat 3061.732 kb on + strand, within Pf1N1B4_2722at 3061.732 kb on + strand, within Pf1N1B4_2722at 3061.732 kb on + strand, within Pf1N1B4_2722at 3061.732 kb on + strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.809 kb on + strand, within Pf1N1B4_2722at 3062.060 kb on + strandat 3062.061 kb on - strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.063 kb on - strandat 3062.063 kb on - strandat 3062.131 kb on + strand, within Pf1N1B4_2723at 3062.132 kb on - strand, within Pf1N1B4_2723at 3062.294 kb on + strandat 3062.294 kb on + strandat 3062.295 kb on - strandat 3062.309 kb on + strandat 3062.309 kb on + strandat 3062.310 kb on - strandat 3062.310 kb on - strandat 3062.310 kb on - strandat 3062.311 kb on + strandat 3062.311 kb on + strandat 3062.312 kb on - strandat 3062.312 kb on - strandat 3062.361 kb on + strandat 3062.361 kb on + strandat 3062.361 kb on + strandat 3062.362 kb on - strandat 3062.362 kb on - strandat 3062.362 kb on - strandat 3062.506 kb on + strand, within Pf1N1B4_2724at 3062.507 kb on - strand, within Pf1N1B4_2724at 3062.507 kb on - strand, within Pf1N1B4_2724at 3062.600 kb on + strand, within Pf1N1B4_2724at 3062.600 kb on + strand, within Pf1N1B4_2724at 3062.601 kb on - strand, within Pf1N1B4_2724at 3062.601 kb on - strand, within Pf1N1B4_2724at 3062.601 kb on - strand, within Pf1N1B4_2724at 3062.613 kb on + strand, within Pf1N1B4_2724at 3062.613 kb on + strand, within Pf1N1B4_2724at 3062.614 kb on - strand, within Pf1N1B4_2724at 3062.704 kb on + strand, within Pf1N1B4_2724at 3062.704 kb on + strand, within Pf1N1B4_2724at 3062.704 kb on + strand, within Pf1N1B4_2724at 3062.705 kb on - strand, within Pf1N1B4_2724at 3062.705 kb on - strand, within Pf1N1B4_2724at 3062.730 kb on + strand, within Pf1N1B4_2724at 3062.730 kb on + strand, within Pf1N1B4_2724at 3062.730 kb on + strand, within Pf1N1B4_2724at 3062.731 kb on - strand, within Pf1N1B4_2724at 3062.731 kb on - strand, within Pf1N1B4_2724at 3062.790 kb on + strand, within Pf1N1B4_2724at 3062.790 kb on + strand, within Pf1N1B4_2724at 3062.790 kb on + strand, within Pf1N1B4_2724at 3062.790 kb on + strand, within Pf1N1B4_2724at 3062.790 kb on + strand, within Pf1N1B4_2724at 3062.790 kb on + strand, within Pf1N1B4_2724at 3062.791 kb on - strand, within Pf1N1B4_2724at 3062.791 kb on - strand, within Pf1N1B4_2724at 3062.791 kb on - strand, within Pf1N1B4_2724at 3062.791 kb on - strand, within Pf1N1B4_2724at 3062.791 kb on - strand, within Pf1N1B4_2724at 3062.891 kb on + strandat 3062.891 kb on + strandat 3062.891 kb on + strandat 3062.892 kb on - strandat 3062.892 kb on - strandat 3062.957 kb on + strandat 3062.971 kb on - strandat 3062.974 kb on + strandat 3063.048 kb on + strandat 3063.054 kb on + strandat 3063.054 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM
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3,060,464 + Pf1N1B4_2721 0.39 +0.8
3,060,465 - Pf1N1B4_2721 0.39 -2.0
3,060,465 - Pf1N1B4_2721 0.39 -1.1
3,060,465 - Pf1N1B4_2721 0.39 +0.0
3,060,515 + Pf1N1B4_2721 0.42 -1.8
3,060,515 + Pf1N1B4_2721 0.42 -2.3
3,060,515 + Pf1N1B4_2721 0.42 -1.0
3,060,516 - Pf1N1B4_2721 0.42 -0.1
3,060,689 + -0.9
3,060,854 + Pf1N1B4_2721 0.66 -2.5
3,060,854 + Pf1N1B4_2721 0.66 -0.7
3,060,872 + Pf1N1B4_2721 0.67 -0.7
3,060,872 + Pf1N1B4_2721 0.67 +0.5
3,060,872 + Pf1N1B4_2721 0.67 -2.1
3,060,872 + Pf1N1B4_2721 0.67 -0.5
3,060,872 + Pf1N1B4_2721 0.67 -1.6
3,060,872 + Pf1N1B4_2721 0.67 -2.3
3,060,872 + Pf1N1B4_2721 0.67 -2.8
3,060,873 - Pf1N1B4_2721 0.67 -2.2
3,060,873 - Pf1N1B4_2721 0.67 -1.4
3,060,873 - Pf1N1B4_2721 0.67 -1.4
3,060,873 - Pf1N1B4_2721 0.67 -1.8
3,061,034 + Pf1N1B4_2721 0.78 -2.6
3,061,034 + Pf1N1B4_2721 0.78 -2.2
3,061,034 + Pf1N1B4_2721 0.78 +0.4
3,061,034 + Pf1N1B4_2721 0.78 -2.7
3,061,035 - Pf1N1B4_2721 0.78 +0.7
3,061,035 - Pf1N1B4_2721 0.78 +1.1
3,061,035 - Pf1N1B4_2721 0.78 -0.7
3,061,235 + +0.5
3,061,235 + -0.1
3,061,236 - -1.4
3,061,236 - -1.8
3,061,236 - +0.7
3,061,236 - -0.7
3,061,236 - -1.7
3,061,390 + +0.7
3,061,412 + +0.8
3,061,413 - -0.7
3,061,732 + Pf1N1B4_2722 0.48 -0.2
3,061,732 + Pf1N1B4_2722 0.48 +0.7
3,061,732 + Pf1N1B4_2722 0.48 +0.1
3,061,732 + Pf1N1B4_2722 0.48 -0.1
3,061,733 - Pf1N1B4_2722 0.48 +1.3
3,061,733 - Pf1N1B4_2722 0.48 +0.0
3,061,733 - Pf1N1B4_2722 0.48 -1.2
3,061,733 - Pf1N1B4_2722 0.48 -1.4
3,061,733 - Pf1N1B4_2722 0.48 +0.2
3,061,733 - Pf1N1B4_2722 0.48 +0.5
3,061,809 + Pf1N1B4_2722 0.60 +0.4
3,062,060 + -0.3
3,062,061 - -0.9
3,062,062 + +0.3
3,062,062 + -0.2
3,062,062 + +1.4
3,062,062 + +0.0
3,062,062 + +1.2
3,062,062 + +0.0
3,062,063 - +0.3
3,062,063 - -0.3
3,062,131 + Pf1N1B4_2723 0.34 -0.5
3,062,132 - Pf1N1B4_2723 0.35 -1.2
3,062,294 + +1.4
3,062,294 + -1.2
3,062,295 - +1.4
3,062,309 + +0.5
3,062,309 + -0.7
3,062,310 - -2.2
3,062,310 - +0.7
3,062,310 - -0.0
3,062,311 + +1.1
3,062,311 + -0.0
3,062,312 - +1.9
3,062,312 - -0.1
3,062,361 + -0.3
3,062,361 + -0.0
3,062,361 + +0.1
3,062,362 - -0.1
3,062,362 - +1.7
3,062,362 - -1.2
3,062,506 + Pf1N1B4_2724 0.10 -1.3
3,062,507 - Pf1N1B4_2724 0.11 -0.9
3,062,507 - Pf1N1B4_2724 0.11 +0.6
3,062,600 + Pf1N1B4_2724 0.30 -0.9
3,062,600 + Pf1N1B4_2724 0.30 -0.2
3,062,601 - Pf1N1B4_2724 0.30 +1.2
3,062,601 - Pf1N1B4_2724 0.30 +1.1
3,062,601 - Pf1N1B4_2724 0.30 -1.1
3,062,613 + Pf1N1B4_2724 0.33 -1.2
3,062,613 + Pf1N1B4_2724 0.33 +0.1
3,062,614 - Pf1N1B4_2724 0.33 -0.2
3,062,704 + Pf1N1B4_2724 0.52 -1.0
3,062,704 + Pf1N1B4_2724 0.52 +0.1
3,062,704 + Pf1N1B4_2724 0.52 +1.4
3,062,705 - Pf1N1B4_2724 0.52 +1.7
3,062,705 - Pf1N1B4_2724 0.52 -0.1
3,062,730 + Pf1N1B4_2724 0.57 -0.9
3,062,730 + Pf1N1B4_2724 0.57 -0.4
3,062,730 + Pf1N1B4_2724 0.57 -0.1
3,062,731 - Pf1N1B4_2724 0.57 -0.8
3,062,731 - Pf1N1B4_2724 0.57 -0.5
3,062,790 + Pf1N1B4_2724 0.70 -0.8
3,062,790 + Pf1N1B4_2724 0.70 -0.6
3,062,790 + Pf1N1B4_2724 0.70 +0.6
3,062,790 + Pf1N1B4_2724 0.70 -0.4
3,062,790 + Pf1N1B4_2724 0.70 +0.8
3,062,790 + Pf1N1B4_2724 0.70 +0.9
3,062,791 - Pf1N1B4_2724 0.70 -0.5
3,062,791 - Pf1N1B4_2724 0.70 +0.1
3,062,791 - Pf1N1B4_2724 0.70 -2.4
3,062,791 - Pf1N1B4_2724 0.70 +0.0
3,062,791 - Pf1N1B4_2724 0.70 -0.1
3,062,891 + -0.0
3,062,891 + -0.8
3,062,891 + +1.0
3,062,892 - -0.5
3,062,892 - -0.1
3,062,957 + -0.9
3,062,971 - -1.8
3,062,974 + -0.3
3,063,048 + +1.0
3,063,054 + -0.6
3,063,054 + -2.0

Or see this region's nucleotide sequence