Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1004 and Pf1N1B4_1005 are separated by 336 nucleotides Pf1N1B4_1005 and Pf1N1B4_1006 are separated by 21 nucleotides Pf1N1B4_1006 and Pf1N1B4_1007 are separated by 283 nucleotides
Pf1N1B4_1004 - Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 1,138,974 to 1,139,948
_1004
Pf1N1B4_1005 - probable membrane protein YPO0899, at 1,140,285 to 1,140,650
_1005
Pf1N1B4_1006 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,140,672 to 1,141,574
_1006
Pf1N1B4_1007 - Transcriptional regulator, AraC family, at 1,141,858 to 1,142,733
_1007
Position (kb)
1140
1141
1142 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1139.760 kb on + strand, within Pf1N1B4_1004 at 1139.994 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.036 kb on - strand at 1140.036 kb on - strand at 1140.036 kb on - strand at 1140.084 kb on - strand at 1140.100 kb on + strand at 1140.203 kb on - strand at 1140.229 kb on - strand at 1140.229 kb on - strand at 1140.229 kb on - strand at 1140.259 kb on - strand at 1140.259 kb on - strand at 1140.264 kb on + strand at 1140.312 kb on + strand at 1140.312 kb on + strand at 1140.313 kb on - strand at 1140.330 kb on - strand, within Pf1N1B4_1005 at 1140.415 kb on - strand, within Pf1N1B4_1005 at 1140.593 kb on + strand, within Pf1N1B4_1005 at 1140.841 kb on + strand at 1140.841 kb on + strand, within Pf1N1B4_1006 at 1140.842 kb on - strand, within Pf1N1B4_1006 at 1140.842 kb on - strand, within Pf1N1B4_1006 at 1140.922 kb on + strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1141.012 kb on + strand, within Pf1N1B4_1006 at 1141.012 kb on + strand, within Pf1N1B4_1006 at 1141.013 kb on - strand, within Pf1N1B4_1006 at 1141.013 kb on - strand, within Pf1N1B4_1006 at 1141.098 kb on + strand, within Pf1N1B4_1006 at 1141.098 kb on + strand, within Pf1N1B4_1006 at 1141.098 kb on + strand, within Pf1N1B4_1006 at 1141.099 kb on - strand, within Pf1N1B4_1006 at 1141.099 kb on - strand, within Pf1N1B4_1006 at 1141.151 kb on + strand, within Pf1N1B4_1006 at 1141.151 kb on + strand, within Pf1N1B4_1006 at 1141.151 kb on + strand, within Pf1N1B4_1006 at 1141.152 kb on - strand at 1141.152 kb on - strand at 1141.331 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.408 kb on - strand, within Pf1N1B4_1006 at 1141.554 kb on + strand at 1141.554 kb on + strand at 1141.554 kb on + strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.559 kb on - strand at 1141.559 kb on - strand at 1141.559 kb on - strand at 1141.559 kb on - strand at 1141.778 kb on - strand at 1141.778 kb on - strand at 1141.778 kb on - strand at 1141.778 kb on - strand at 1141.828 kb on + strand at 1141.829 kb on - strand at 1141.990 kb on + strand, within Pf1N1B4_1007 at 1142.023 kb on + strand, within Pf1N1B4_1007 at 1142.023 kb on + strand, within Pf1N1B4_1007 at 1142.023 kb on + strand, within Pf1N1B4_1007 at 1142.024 kb on - strand, within Pf1N1B4_1007 at 1142.024 kb on - strand, within Pf1N1B4_1007 at 1142.024 kb on - strand, within Pf1N1B4_1007 at 1142.024 kb on - strand, within Pf1N1B4_1007 at 1142.024 kb on - strand, within Pf1N1B4_1007 at 1142.024 kb on - strand, within Pf1N1B4_1007 at 1142.024 kb on - strand, within Pf1N1B4_1007 at 1142.044 kb on - strand, within Pf1N1B4_1007 at 1142.158 kb on + strand, within Pf1N1B4_1007 at 1142.492 kb on + strand, within Pf1N1B4_1007 at 1142.493 kb on - strand, within Pf1N1B4_1007 at 1142.560 kb on + strand, within Pf1N1B4_1007 at 1142.561 kb on - strand, within Pf1N1B4_1007 at 1142.561 kb on - strand, within Pf1N1B4_1007 at 1142.561 kb on - strand, within Pf1N1B4_1007 at 1142.561 kb on - strand, within Pf1N1B4_1007 at 1142.561 kb on - strand, within Pf1N1B4_1007 at 1142.561 kb on - strand, within Pf1N1B4_1007 at 1142.561 kb on - strand, within Pf1N1B4_1007 at 1142.561 kb on - strand, within Pf1N1B4_1007
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 1,139,760 + Pf1N1B4_1004 0.81 -1.4 1,139,994 - -0.5 1,140,020 - +0.6 1,140,020 - -0.7 1,140,020 - -0.5 1,140,020 - -0.2 1,140,036 - +1.2 1,140,036 - -2.1 1,140,036 - +0.1 1,140,084 - +0.7 1,140,100 + -1.1 1,140,203 - -2.0 1,140,229 - -0.3 1,140,229 - -0.4 1,140,229 - +0.1 1,140,259 - +0.7 1,140,259 - -0.3 1,140,264 + +1.1 1,140,312 + +0.5 1,140,312 + -1.4 1,140,313 - -0.3 1,140,330 - Pf1N1B4_1005 0.12 +1.1 1,140,415 - Pf1N1B4_1005 0.36 +0.1 1,140,593 + Pf1N1B4_1005 0.84 +0.1 1,140,841 + -0.4 1,140,841 + Pf1N1B4_1006 0.19 -0.7 1,140,842 - Pf1N1B4_1006 0.19 -2.4 1,140,842 - Pf1N1B4_1006 0.19 +0.9 1,140,922 + Pf1N1B4_1006 0.28 -2.9 1,140,923 - Pf1N1B4_1006 0.28 -1.9 1,140,923 - Pf1N1B4_1006 0.28 -3.0 1,140,923 - Pf1N1B4_1006 0.28 -0.3 1,140,940 + Pf1N1B4_1006 0.30 -2.8 1,140,940 + Pf1N1B4_1006 0.30 -2.2 1,140,940 + Pf1N1B4_1006 0.30 -2.7 1,140,940 + Pf1N1B4_1006 0.30 -0.3 1,141,012 + Pf1N1B4_1006 0.38 -3.4 1,141,012 + Pf1N1B4_1006 0.38 -0.1 1,141,013 - Pf1N1B4_1006 0.38 -2.4 1,141,013 - Pf1N1B4_1006 0.38 -1.2 1,141,098 + Pf1N1B4_1006 0.47 -1.0 1,141,098 + Pf1N1B4_1006 0.47 -1.2 1,141,098 + Pf1N1B4_1006 0.47 -1.2 1,141,099 - Pf1N1B4_1006 0.47 -0.6 1,141,099 - Pf1N1B4_1006 0.47 -1.2 1,141,151 + Pf1N1B4_1006 0.53 -2.3 1,141,151 + Pf1N1B4_1006 0.53 -2.9 1,141,151 + Pf1N1B4_1006 0.53 -2.9 1,141,152 - -1.2 1,141,152 - -1.9 1,141,331 + Pf1N1B4_1006 0.73 -3.7 1,141,407 + Pf1N1B4_1006 0.81 -0.4 1,141,407 + Pf1N1B4_1006 0.81 -2.2 1,141,407 + Pf1N1B4_1006 0.81 -0.8 1,141,407 + Pf1N1B4_1006 0.81 -2.6 1,141,407 + Pf1N1B4_1006 0.81 -1.8 1,141,408 - Pf1N1B4_1006 0.82 -2.1 1,141,554 + -3.3 1,141,554 + -1.9 1,141,554 + -3.7 1,141,555 - -0.9 1,141,555 - -1.4 1,141,555 - -1.4 1,141,555 - -2.2 1,141,555 - -1.9 1,141,555 - -2.4 1,141,558 + -3.5 1,141,558 + +0.6 1,141,558 + -0.0 1,141,558 + -2.2 1,141,558 + +0.4 1,141,558 + -1.0 1,141,558 + +0.8 1,141,559 - -1.0 1,141,559 - -2.0 1,141,559 - -0.4 1,141,559 - -3.2 1,141,778 - +0.9 1,141,778 - -1.6 1,141,778 - -0.3 1,141,778 - -2.5 1,141,828 + +0.5 1,141,829 - +0.6 1,141,990 + Pf1N1B4_1007 0.15 -1.8 1,142,023 + Pf1N1B4_1007 0.19 -2.4 1,142,023 + Pf1N1B4_1007 0.19 +1.5 1,142,023 + Pf1N1B4_1007 0.19 -2.8 1,142,024 - Pf1N1B4_1007 0.19 +0.2 1,142,024 - Pf1N1B4_1007 0.19 -1.3 1,142,024 - Pf1N1B4_1007 0.19 -0.3 1,142,024 - Pf1N1B4_1007 0.19 -2.0 1,142,024 - Pf1N1B4_1007 0.19 +0.2 1,142,024 - Pf1N1B4_1007 0.19 +0.2 1,142,024 - Pf1N1B4_1007 0.19 -0.4 1,142,044 - Pf1N1B4_1007 0.21 +0.8 1,142,158 + Pf1N1B4_1007 0.34 -2.6 1,142,492 + Pf1N1B4_1007 0.72 -2.2 1,142,493 - Pf1N1B4_1007 0.72 -1.1 1,142,560 + Pf1N1B4_1007 0.80 -2.5 1,142,561 - Pf1N1B4_1007 0.80 -2.4 1,142,561 - Pf1N1B4_1007 0.80 -1.8 1,142,561 - Pf1N1B4_1007 0.80 -1.7 1,142,561 - Pf1N1B4_1007 0.80 -1.8 1,142,561 - Pf1N1B4_1007 0.80 -3.0 1,142,561 - Pf1N1B4_1007 0.80 -0.1 1,142,561 - Pf1N1B4_1007 0.80 -0.6 1,142,561 - Pf1N1B4_1007 0.80 -2.0
Or see this region's nucleotide sequence