Experiment: KB with 2,5-Dihydroxybenzoic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1565 and Psyr_1566 are separated by 66 nucleotides Psyr_1566 and Psyr_1567 are separated by 210 nucleotides Psyr_1567 and Psyr_1568 are separated by 4 nucleotides
Psyr_1565: Psyr_1565 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 1,761,509 to 1,762,441
_1565
Psyr_1566: Psyr_1566 - Nitroreductase, at 1,762,508 to 1,763,101
_1566
Psyr_1567: Psyr_1567 - Uncharacterized conserved protein UCP006173, at 1,763,312 to 1,763,761
_1567
Psyr_1568: Psyr_1568 - conserved hypothetical protein, at 1,763,766 to 1,764,146
_1568
Position (kb)
1762
1763
1764 Strain fitness (log2 ratio)
-2
-1
0
1 at 1761.545 kb on + strand at 1761.545 kb on + strand at 1761.545 kb on + strand at 1761.545 kb on + strand at 1761.546 kb on - strand at 1761.546 kb on - strand at 1761.546 kb on - strand at 1761.546 kb on - strand at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.989 kb on + strand, within Psyr_1565 at 1761.990 kb on - strand, within Psyr_1565 at 1761.990 kb on - strand, within Psyr_1565 at 1762.079 kb on + strand, within Psyr_1565 at 1762.079 kb on + strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.304 kb on + strand, within Psyr_1565 at 1762.304 kb on + strand, within Psyr_1565 at 1762.304 kb on + strand, within Psyr_1565 at 1762.510 kb on - strand at 1762.584 kb on + strand, within Psyr_1566 at 1762.584 kb on + strand, within Psyr_1566 at 1762.657 kb on + strand, within Psyr_1566 at 1762.658 kb on - strand, within Psyr_1566 at 1762.743 kb on + strand, within Psyr_1566 at 1762.897 kb on - strand, within Psyr_1566 at 1762.929 kb on + strand, within Psyr_1566 at 1762.929 kb on + strand, within Psyr_1566 at 1762.930 kb on - strand, within Psyr_1566 at 1762.930 kb on - strand, within Psyr_1566 at 1762.954 kb on + strand, within Psyr_1566 at 1762.956 kb on + strand, within Psyr_1566 at 1762.956 kb on + strand, within Psyr_1566 at 1762.956 kb on + strand, within Psyr_1566 at 1762.957 kb on - strand, within Psyr_1566 at 1762.957 kb on - strand, within Psyr_1566 at 1763.196 kb on - strand at 1763.264 kb on + strand at 1763.264 kb on + strand at 1763.265 kb on - strand at 1763.270 kb on + strand at 1763.271 kb on - strand at 1763.447 kb on + strand, within Psyr_1567 at 1763.450 kb on + strand, within Psyr_1567 at 1763.450 kb on + strand, within Psyr_1567 at 1763.450 kb on + strand, within Psyr_1567 at 1763.450 kb on + strand, within Psyr_1567 at 1763.677 kb on + strand, within Psyr_1567 at 1763.677 kb on + strand, within Psyr_1567 at 1763.677 kb on + strand, within Psyr_1567 at 1763.738 kb on + strand at 1763.739 kb on - strand at 1763.767 kb on + strand at 1763.767 kb on + strand at 1763.767 kb on + strand at 1763.768 kb on - strand at 1763.768 kb on - strand at 1763.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with 2,5-Dihydroxybenzoic Acid 10 mM remove 1,761,545 + +0.3 1,761,545 + +0.3 1,761,545 + +1.1 1,761,545 + +0.7 1,761,546 - -0.0 1,761,546 - -0.6 1,761,546 - -0.4 1,761,546 - +1.2 1,761,818 + Psyr_1565 0.33 -0.7 1,761,818 + Psyr_1565 0.33 +0.6 1,761,818 + Psyr_1565 0.33 -0.9 1,761,818 + Psyr_1565 0.33 -0.2 1,761,818 + Psyr_1565 0.33 +0.2 1,761,818 + Psyr_1565 0.33 -0.4 1,761,818 + Psyr_1565 0.33 +0.3 1,761,818 + Psyr_1565 0.33 -0.4 1,761,819 - Psyr_1565 0.33 +1.0 1,761,819 - Psyr_1565 0.33 +0.4 1,761,819 - Psyr_1565 0.33 +1.3 1,761,819 - Psyr_1565 0.33 -0.8 1,761,819 - Psyr_1565 0.33 +0.7 1,761,989 + Psyr_1565 0.51 +0.4 1,761,990 - Psyr_1565 0.52 -0.6 1,761,990 - Psyr_1565 0.52 +0.7 1,762,079 + Psyr_1565 0.61 -0.0 1,762,079 + Psyr_1565 0.61 +1.0 1,762,080 - Psyr_1565 0.61 -1.8 1,762,080 - Psyr_1565 0.61 -0.2 1,762,080 - Psyr_1565 0.61 +0.3 1,762,080 - Psyr_1565 0.61 +0.0 1,762,080 - Psyr_1565 0.61 +0.4 1,762,080 - Psyr_1565 0.61 +0.0 1,762,304 + Psyr_1565 0.85 -0.4 1,762,304 + Psyr_1565 0.85 +0.0 1,762,304 + Psyr_1565 0.85 +0.5 1,762,510 - +0.0 1,762,584 + Psyr_1566 0.13 -0.0 1,762,584 + Psyr_1566 0.13 -1.1 1,762,657 + Psyr_1566 0.25 -0.5 1,762,658 - Psyr_1566 0.25 -0.7 1,762,743 + Psyr_1566 0.40 +0.6 1,762,897 - Psyr_1566 0.65 -0.0 1,762,929 + Psyr_1566 0.71 -1.0 1,762,929 + Psyr_1566 0.71 -0.3 1,762,930 - Psyr_1566 0.71 +0.1 1,762,930 - Psyr_1566 0.71 -1.5 1,762,954 + Psyr_1566 0.75 +1.0 1,762,956 + Psyr_1566 0.75 -0.9 1,762,956 + Psyr_1566 0.75 -0.0 1,762,956 + Psyr_1566 0.75 +0.5 1,762,957 - Psyr_1566 0.76 +0.4 1,762,957 - Psyr_1566 0.76 -1.0 1,763,196 - -0.2 1,763,264 + -0.8 1,763,264 + +0.3 1,763,265 - -0.3 1,763,270 + +1.3 1,763,271 - -0.8 1,763,447 + Psyr_1567 0.30 +0.4 1,763,450 + Psyr_1567 0.31 +0.2 1,763,450 + Psyr_1567 0.31 +0.2 1,763,450 + Psyr_1567 0.31 +1.0 1,763,450 + Psyr_1567 0.31 -1.0 1,763,677 + Psyr_1567 0.81 -0.4 1,763,677 + Psyr_1567 0.81 +0.3 1,763,677 + Psyr_1567 0.81 +0.8 1,763,738 + -0.6 1,763,739 - +0.5 1,763,767 + +0.7 1,763,767 + -0.2 1,763,767 + -0.3 1,763,768 - +0.2 1,763,768 - -0.3 1,763,768 - -0.0
Or see this region's nucleotide sequence