Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4934

Experiment: nitrogen source nitrate 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4932 and Pf1N1B4_4933 are separated by 171 nucleotidesPf1N1B4_4933 and Pf1N1B4_4934 are separated by 224 nucleotidesPf1N1B4_4934 and Pf1N1B4_4935 overlap by 4 nucleotides Pf1N1B4_4932 - Tartrate dehydrogenase (EC 1.1.1.93) / Tartrate decarboxylase (EC 4.1.1.73) / D-malic enzyme (EC 1.1.1.83), at 5,466,700 to 5,467,779 _4932 Pf1N1B4_4933 - FIG00955097: hypothetical protein, at 5,467,951 to 5,468,175 _4933 Pf1N1B4_4934 - DNA damage response nuclease (from data), at 5,468,400 to 5,470,052 _4934 Pf1N1B4_4935 - DinG family ATP-dependent helicase CPE1197, at 5,470,049 to 5,472,328 _4935 Position (kb) 5468 5469 5470 5471Strain fitness (log2 ratio) -2 -1 0 1 2at 5467.567 kb on + strand, within Pf1N1B4_4932at 5467.567 kb on + strand, within Pf1N1B4_4932at 5467.568 kb on - strand, within Pf1N1B4_4932at 5467.926 kb on - strandat 5468.072 kb on + strandat 5468.175 kb on + strandat 5468.176 kb on - strandat 5468.347 kb on - strandat 5468.347 kb on - strandat 5468.347 kb on - strandat 5468.353 kb on + strandat 5468.408 kb on - strandat 5468.408 kb on - strandat 5468.488 kb on + strandat 5468.610 kb on + strand, within Pf1N1B4_4934at 5468.611 kb on - strand, within Pf1N1B4_4934at 5468.963 kb on + strand, within Pf1N1B4_4934at 5468.964 kb on - strand, within Pf1N1B4_4934at 5469.349 kb on + strand, within Pf1N1B4_4934at 5469.519 kb on + strand, within Pf1N1B4_4934at 5469.519 kb on + strandat 5469.520 kb on - strand, within Pf1N1B4_4934at 5469.705 kb on + strand, within Pf1N1B4_4934at 5469.706 kb on - strand, within Pf1N1B4_4934at 5469.706 kb on - strand, within Pf1N1B4_4934at 5469.730 kb on - strandat 5469.764 kb on - strandat 5470.055 kb on + strandat 5470.295 kb on + strand, within Pf1N1B4_4935at 5470.296 kb on - strandat 5470.361 kb on + strand, within Pf1N1B4_4935at 5470.479 kb on + strandat 5470.479 kb on + strand, within Pf1N1B4_4935at 5470.929 kb on - strandat 5471.031 kb on - strandat 5471.031 kb on - strand, within Pf1N1B4_4935

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM
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5,467,567 + Pf1N1B4_4932 0.80 -0.7
5,467,567 + Pf1N1B4_4932 0.80 -2.4
5,467,568 - Pf1N1B4_4932 0.80 +0.3
5,467,926 - +0.3
5,468,072 + -1.4
5,468,175 + -1.6
5,468,176 - -0.0
5,468,347 - +0.2
5,468,347 - -1.4
5,468,347 - -0.1
5,468,353 + -1.5
5,468,408 - +1.3
5,468,408 - +0.6
5,468,488 + -1.3
5,468,610 + Pf1N1B4_4934 0.13 -0.9
5,468,611 - Pf1N1B4_4934 0.13 +1.1
5,468,963 + Pf1N1B4_4934 0.34 +0.1
5,468,964 - Pf1N1B4_4934 0.34 +1.1
5,469,349 + Pf1N1B4_4934 0.57 +1.3
5,469,519 + Pf1N1B4_4934 0.68 -0.0
5,469,519 + -0.1
5,469,520 - Pf1N1B4_4934 0.68 +0.3
5,469,705 + Pf1N1B4_4934 0.79 +0.9
5,469,706 - Pf1N1B4_4934 0.79 +0.6
5,469,706 - Pf1N1B4_4934 0.79 +1.8
5,469,730 - +1.6
5,469,764 - +1.2
5,470,055 + +1.0
5,470,295 + Pf1N1B4_4935 0.11 -0.9
5,470,296 - +0.3
5,470,361 + Pf1N1B4_4935 0.14 -1.4
5,470,479 + -1.8
5,470,479 + Pf1N1B4_4935 0.19 -0.3
5,470,929 - -0.7
5,471,031 - -0.6
5,471,031 - Pf1N1B4_4935 0.43 -0.6

Or see this region's nucleotide sequence