Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_491 and Pf1N1B4_492 overlap by 4 nucleotides Pf1N1B4_492 and Pf1N1B4_493 are separated by 18 nucleotides Pf1N1B4_493 and Pf1N1B4_494 are separated by 735 nucleotides
Pf1N1B4_491 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 583,248 to 584,471
_491
Pf1N1B4_492 - Flp pilus assembly protein TadD, contains TPR repeats, at 584,468 to 585,295
_492
Pf1N1B4_493 - hypothetical protein, at 585,314 to 585,583
_493
Pf1N1B4_494 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 586,319 to 587,281
_494
Position (kb) 585
586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 584.365 kb on + strand at 584.365 kb on + strand at 584.365 kb on + strand at 584.366 kb on - strand at 584.366 kb on - strand at 584.366 kb on - strand at 584.397 kb on + strand at 584.398 kb on - strand at 584.398 kb on - strand at 584.569 kb on - strand, within Pf1N1B4_492 at 584.663 kb on - strand, within Pf1N1B4_492 at 584.738 kb on + strand, within Pf1N1B4_492 at 584.739 kb on - strand, within Pf1N1B4_492 at 584.846 kb on + strand, within Pf1N1B4_492 at 584.846 kb on + strand, within Pf1N1B4_492 at 584.847 kb on - strand, within Pf1N1B4_492 at 585.008 kb on + strand, within Pf1N1B4_492 at 585.009 kb on - strand, within Pf1N1B4_492 at 585.025 kb on + strand, within Pf1N1B4_492 at 585.025 kb on + strand, within Pf1N1B4_492 at 585.025 kb on + strand, within Pf1N1B4_492 at 585.026 kb on - strand, within Pf1N1B4_492 at 585.098 kb on - strand, within Pf1N1B4_492 at 585.116 kb on + strand, within Pf1N1B4_492 at 585.117 kb on - strand at 585.117 kb on - strand, within Pf1N1B4_492 at 585.254 kb on - strand at 585.254 kb on - strand at 585.293 kb on + strand at 585.294 kb on - strand at 585.294 kb on - strand at 585.294 kb on - strand at 585.301 kb on - strand at 585.319 kb on + strand at 585.429 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.445 kb on - strand, within Pf1N1B4_493 at 585.445 kb on - strand, within Pf1N1B4_493 at 585.445 kb on - strand, within Pf1N1B4_493 at 585.619 kb on + strand at 585.664 kb on + strand at 585.664 kb on + strand at 585.664 kb on + strand at 585.664 kb on + strand at 585.664 kb on + strand at 585.665 kb on - strand at 585.665 kb on - strand at 585.710 kb on + strand at 585.799 kb on + strand at 585.799 kb on + strand at 585.913 kb on + strand at 585.927 kb on + strand at 585.927 kb on + strand at 585.927 kb on + strand at 585.928 kb on - strand at 586.012 kb on + strand at 586.012 kb on + strand at 586.013 kb on - strand at 586.034 kb on + strand at 586.034 kb on + strand at 586.035 kb on - strand at 586.035 kb on - strand at 586.124 kb on + strand at 586.124 kb on + strand at 586.124 kb on + strand at 586.124 kb on + strand at 586.125 kb on - strand at 586.125 kb on - strand at 586.125 kb on - strand at 586.125 kb on - strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.127 kb on - strand at 586.127 kb on - strand at 586.127 kb on - strand at 586.127 kb on - strand at 586.140 kb on + strand at 586.235 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.294 kb on + strand at 586.294 kb on + strand at 586.406 kb on + strand at 586.407 kb on - strand at 586.407 kb on - strand at 586.453 kb on + strand, within Pf1N1B4_494 at 586.479 kb on + strand, within Pf1N1B4_494 at 586.479 kb on + strand, within Pf1N1B4_494 at 586.479 kb on + strand, within Pf1N1B4_494 at 586.479 kb on + strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.520 kb on - strand, within Pf1N1B4_494 at 586.550 kb on + strand, within Pf1N1B4_494 at 586.550 kb on + strand, within Pf1N1B4_494 at 586.550 kb on + strand, within Pf1N1B4_494 at 586.551 kb on - strand at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 584,365 + -1.0 584,365 + +1.1 584,365 + -1.0 584,366 - +0.8 584,366 - -0.1 584,366 - -0.5 584,397 + -0.6 584,398 - -0.1 584,398 - +1.9 584,569 - Pf1N1B4_492 0.12 -0.0 584,663 - Pf1N1B4_492 0.24 +0.1 584,738 + Pf1N1B4_492 0.33 -0.4 584,739 - Pf1N1B4_492 0.33 -0.7 584,846 + Pf1N1B4_492 0.46 +0.9 584,846 + Pf1N1B4_492 0.46 +3.5 584,847 - Pf1N1B4_492 0.46 -0.6 585,008 + Pf1N1B4_492 0.65 +0.9 585,009 - Pf1N1B4_492 0.65 +0.0 585,025 + Pf1N1B4_492 0.67 +0.2 585,025 + Pf1N1B4_492 0.67 -0.4 585,025 + Pf1N1B4_492 0.67 -0.5 585,026 - Pf1N1B4_492 0.67 -3.4 585,098 - Pf1N1B4_492 0.76 +1.4 585,116 + Pf1N1B4_492 0.78 -0.3 585,117 - -1.2 585,117 - Pf1N1B4_492 0.78 -1.3 585,254 - -0.3 585,254 - -0.4 585,293 + +1.8 585,294 - -0.6 585,294 - +0.1 585,294 - +0.9 585,301 - -0.3 585,319 + -1.9 585,429 + Pf1N1B4_493 0.43 -0.4 585,444 + Pf1N1B4_493 0.48 +3.0 585,444 + Pf1N1B4_493 0.48 +0.8 585,444 + Pf1N1B4_493 0.48 +1.3 585,444 + +1.5 585,444 + Pf1N1B4_493 0.48 +1.6 585,444 + Pf1N1B4_493 0.48 -0.7 585,445 - Pf1N1B4_493 0.49 -0.7 585,445 - Pf1N1B4_493 0.49 -1.0 585,445 - Pf1N1B4_493 0.49 -1.1 585,619 + +0.2 585,664 + +0.6 585,664 + +1.8 585,664 + +0.7 585,664 + +1.0 585,664 + +2.2 585,665 - -1.0 585,665 - -0.0 585,710 + -0.4 585,799 + +0.9 585,799 + +0.6 585,913 + -0.1 585,927 + +1.1 585,927 + +0.2 585,927 + -1.1 585,928 - +0.7 586,012 + +1.6 586,012 + -1.0 586,013 - -0.2 586,034 + +0.5 586,034 + +0.1 586,035 - -0.3 586,035 - -0.3 586,124 + -0.2 586,124 + +0.8 586,124 + -0.0 586,124 + +1.0 586,125 - -0.8 586,125 - -1.2 586,125 - -0.7 586,125 - +2.3 586,126 + -1.0 586,126 + -0.0 586,126 + -1.3 586,126 + -1.1 586,126 + +0.5 586,126 + +0.9 586,126 + -1.7 586,126 + +0.3 586,126 + -0.3 586,127 - +0.8 586,127 - +2.8 586,127 - +0.4 586,127 - -0.7 586,140 + -1.2 586,235 + +0.0 586,251 + +0.1 586,251 + -0.4 586,251 + +1.3 586,251 + -0.2 586,251 + +0.5 586,251 + +0.8 586,251 + -1.3 586,251 + -1.5 586,251 + -0.3 586,251 + -1.9 586,251 + +0.6 586,252 - +0.3 586,252 - +1.8 586,252 - +0.5 586,252 - -2.4 586,252 - +1.5 586,252 - +0.7 586,252 - -2.0 586,294 + -0.7 586,294 + +0.6 586,406 + +1.1 586,407 - +0.8 586,407 - +0.6 586,453 + Pf1N1B4_494 0.14 -0.6 586,479 + Pf1N1B4_494 0.17 +0.3 586,479 + Pf1N1B4_494 0.17 -0.0 586,479 + Pf1N1B4_494 0.17 +0.8 586,479 + Pf1N1B4_494 0.17 +1.4 586,480 - Pf1N1B4_494 0.17 +0.9 586,480 - Pf1N1B4_494 0.17 -2.2 586,480 - Pf1N1B4_494 0.17 +0.5 586,480 - Pf1N1B4_494 0.17 +0.6 586,480 - Pf1N1B4_494 0.17 +1.5 586,520 - Pf1N1B4_494 0.21 -1.7 586,550 + Pf1N1B4_494 0.24 -0.4 586,550 + Pf1N1B4_494 0.24 -1.4 586,550 + Pf1N1B4_494 0.24 +0.1 586,551 - -1.8 586,551 - Pf1N1B4_494 0.24 -0.4 586,551 - Pf1N1B4_494 0.24 +0.8 586,551 - Pf1N1B4_494 0.24 +0.0 586,551 - Pf1N1B4_494 0.24 +0.4 586,551 - Pf1N1B4_494 0.24 -0.6 586,551 - Pf1N1B4_494 0.24 +0.4
Or see this region's nucleotide sequence