Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4440 and Pf1N1B4_4441 are separated by 176 nucleotides Pf1N1B4_4441 and Pf1N1B4_4442 are separated by 117 nucleotides Pf1N1B4_4442 and Pf1N1B4_4443 overlap by 15 nucleotides Pf1N1B4_4443 and Pf1N1B4_4444 are separated by 210 nucleotides
Pf1N1B4_4440 - Carbonic anhydrase (EC 4.2.1.1), at 4,900,532 to 4,901,191
_4440
Pf1N1B4_4441 - Cyanate hydratase (EC 4.2.1.104), at 4,901,368 to 4,901,835
_4441
Pf1N1B4_4442 - Cyn operon transcriptional activator, at 4,901,953 to 4,902,858
_4442
Pf1N1B4_4443 - hypothetical protein, at 4,902,844 to 4,903,059
_4443
Pf1N1B4_4444 - FIG00960995: hypothetical protein, at 4,903,270 to 4,903,617
_4444
Position (kb)
4901
4902
4903 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4900.984 kb on - strand, within Pf1N1B4_4440 at 4901.134 kb on + strand at 4901.236 kb on + strand at 4901.528 kb on + strand, within Pf1N1B4_4441 at 4901.555 kb on + strand, within Pf1N1B4_4441 at 4901.711 kb on + strand, within Pf1N1B4_4441 at 4901.711 kb on + strand, within Pf1N1B4_4441 at 4901.816 kb on + strand at 4901.816 kb on + strand at 4901.913 kb on - strand at 4901.913 kb on - strand at 4901.913 kb on - strand at 4901.913 kb on - strand at 4901.918 kb on - strand at 4901.918 kb on - strand at 4901.918 kb on - strand at 4901.918 kb on - strand at 4901.926 kb on + strand at 4901.937 kb on + strand at 4901.939 kb on + strand at 4901.939 kb on + strand at 4901.939 kb on + strand at 4901.940 kb on - strand at 4901.940 kb on - strand at 4901.975 kb on - strand at 4902.142 kb on + strand, within Pf1N1B4_4442 at 4902.142 kb on + strand, within Pf1N1B4_4442 at 4902.142 kb on + strand, within Pf1N1B4_4442 at 4902.142 kb on + strand at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand at 4902.145 kb on - strand, within Pf1N1B4_4442 at 4902.262 kb on + strand, within Pf1N1B4_4442 at 4902.263 kb on - strand, within Pf1N1B4_4442 at 4902.289 kb on + strand at 4902.289 kb on + strand, within Pf1N1B4_4442 at 4902.478 kb on + strand, within Pf1N1B4_4442 at 4902.574 kb on + strand, within Pf1N1B4_4442 at 4902.775 kb on + strand at 4902.775 kb on + strand at 4902.776 kb on - strand at 4902.776 kb on - strand at 4902.776 kb on - strand at 4903.184 kb on + strand at 4903.184 kb on + strand at 4903.184 kb on + strand at 4903.184 kb on + strand at 4903.185 kb on - strand at 4903.190 kb on + strand at 4903.190 kb on + strand at 4903.191 kb on - strand at 4903.256 kb on - strand at 4903.269 kb on - strand at 4903.275 kb on - strand at 4903.275 kb on - strand at 4903.275 kb on - strand at 4903.275 kb on - strand at 4903.391 kb on + strand, within Pf1N1B4_4444 at 4903.392 kb on - strand, within Pf1N1B4_4444 at 4903.522 kb on - strand, within Pf1N1B4_4444 at 4903.602 kb on + strand at 4903.603 kb on - strand at 4903.647 kb on + strand at 4903.648 kb on - strand at 4903.665 kb on + strand at 4903.742 kb on - strand at 4903.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 4,900,984 - Pf1N1B4_4440 0.68 +0.4 4,901,134 + +0.6 4,901,236 + -1.0 4,901,528 + Pf1N1B4_4441 0.34 +0.4 4,901,555 + Pf1N1B4_4441 0.40 +0.7 4,901,711 + Pf1N1B4_4441 0.73 -0.6 4,901,711 + Pf1N1B4_4441 0.73 -0.2 4,901,816 + +1.6 4,901,816 + +0.4 4,901,913 - +0.8 4,901,913 - +0.7 4,901,913 - -1.3 4,901,913 - -0.4 4,901,918 - +0.5 4,901,918 - -1.0 4,901,918 - -0.3 4,901,918 - -0.0 4,901,926 + +0.3 4,901,937 + +0.6 4,901,939 + +0.4 4,901,939 + +2.1 4,901,939 + +0.7 4,901,940 - -0.5 4,901,940 - +2.4 4,901,975 - +0.4 4,902,142 + Pf1N1B4_4442 0.21 +0.4 4,902,142 + Pf1N1B4_4442 0.21 +0.5 4,902,142 + Pf1N1B4_4442 0.21 -0.1 4,902,142 + +0.6 4,902,143 - Pf1N1B4_4442 0.21 +0.1 4,902,143 - Pf1N1B4_4442 0.21 +1.2 4,902,143 - Pf1N1B4_4442 0.21 +0.3 4,902,143 - Pf1N1B4_4442 0.21 +0.1 4,902,143 - Pf1N1B4_4442 0.21 +1.4 4,902,143 - -0.2 4,902,145 - Pf1N1B4_4442 0.21 -0.1 4,902,262 + Pf1N1B4_4442 0.34 +0.6 4,902,263 - Pf1N1B4_4442 0.34 -0.8 4,902,289 + -1.2 4,902,289 + Pf1N1B4_4442 0.37 +0.8 4,902,478 + Pf1N1B4_4442 0.58 -0.2 4,902,574 + Pf1N1B4_4442 0.69 -2.3 4,902,775 + +0.8 4,902,775 + -2.0 4,902,776 - -0.5 4,902,776 - +0.4 4,902,776 - +0.2 4,903,184 + -0.2 4,903,184 + -0.3 4,903,184 + -0.2 4,903,184 + +0.1 4,903,185 - -0.5 4,903,190 + +1.0 4,903,190 + +0.2 4,903,191 - -3.1 4,903,256 - -0.4 4,903,269 - -2.6 4,903,275 - -0.2 4,903,275 - +1.2 4,903,275 - -0.2 4,903,275 - -0.4 4,903,391 + Pf1N1B4_4444 0.35 -1.6 4,903,392 - Pf1N1B4_4444 0.35 -1.4 4,903,522 - Pf1N1B4_4444 0.72 -0.4 4,903,602 + -0.8 4,903,603 - -1.8 4,903,647 + +0.6 4,903,648 - +0.1 4,903,665 + +1.7 4,903,742 - +1.9 4,903,742 - +1.1
Or see this region's nucleotide sequence