Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4761 and BT4762 are separated by 36 nucleotides BT4762 and BT4763 are separated by 2 nucleotides BT4763 and BT4764 are separated by 3 nucleotides
BT4761: BT4761 - conserved protein found in conjugate transposon (NCBI ptt file), at 6,248,072 to 6,248,212
BT4761
BT4762: BT4762 - conserved protein found in conjugate transposon (NCBI ptt file), at 6,248,249 to 6,248,686
BT4762
BT4763: BT4763 - conserved protein found in conjugate transposon (NCBI ptt file), at 6,248,689 to 6,249,183
BT4763
BT4764: BT4764 - hypothetical protein (NCBI ptt file), at 6,249,187 to 6,250,119
BT4764
Position (kb)
6248
6249
6250 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 6247.696 kb on + strand at 6247.743 kb on - strand at 6247.743 kb on - strand at 6247.747 kb on - strand at 6247.759 kb on - strand at 6247.767 kb on - strand at 6247.771 kb on - strand at 6247.791 kb on - strand at 6247.794 kb on + strand at 6247.797 kb on - strand at 6247.807 kb on - strand at 6247.811 kb on - strand at 6247.811 kb on - strand at 6247.871 kb on - strand at 6247.895 kb on - strand at 6247.907 kb on + strand at 6248.016 kb on - strand at 6248.016 kb on - strand at 6248.072 kb on - strand at 6248.072 kb on - strand at 6248.239 kb on - strand at 6248.239 kb on - strand at 6248.306 kb on - strand, within BT4762 at 6248.437 kb on - strand, within BT4762 at 6248.478 kb on - strand, within BT4762 at 6248.478 kb on - strand, within BT4762 at 6248.478 kb on - strand, within BT4762 at 6248.478 kb on - strand, within BT4762 at 6248.534 kb on - strand, within BT4762 at 6248.651 kb on - strand at 6248.651 kb on - strand at 6248.665 kb on - strand at 6248.989 kb on + strand, within BT4763 at 6248.992 kb on - strand, within BT4763 at 6249.017 kb on - strand, within BT4763 at 6249.019 kb on - strand, within BT4763 at 6249.181 kb on + strand at 6249.192 kb on + strand at 6249.208 kb on + strand at 6249.209 kb on - strand at 6249.262 kb on - strand at 6249.630 kb on + strand, within BT4764 at 6249.678 kb on + strand, within BT4764 at 6249.679 kb on - strand, within BT4764 at 6249.905 kb on + strand, within BT4764 at 6250.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 6,247,696 + -0.5 6,247,743 - +0.8 6,247,743 - -3.9 6,247,747 - -1.8 6,247,759 - -2.1 6,247,767 - +0.2 6,247,771 - -2.6 6,247,791 - +1.1 6,247,794 + -1.7 6,247,797 - -2.1 6,247,807 - -2.9 6,247,811 - -0.7 6,247,811 - -2.4 6,247,871 - +0.3 6,247,895 - -2.6 6,247,907 + +0.9 6,248,016 - -0.4 6,248,016 - +0.7 6,248,072 - -1.9 6,248,072 - -0.1 6,248,239 - -1.9 6,248,239 - -0.4 6,248,306 - BT4762 0.13 +0.9 6,248,437 - BT4762 0.43 -0.9 6,248,478 - BT4762 0.52 -2.1 6,248,478 - BT4762 0.52 -0.8 6,248,478 - BT4762 0.52 -1.3 6,248,478 - BT4762 0.52 -2.3 6,248,534 - BT4762 0.65 -2.6 6,248,651 - -1.9 6,248,651 - -0.1 6,248,665 - -0.2 6,248,989 + BT4763 0.61 -2.1 6,248,992 - BT4763 0.61 -0.1 6,249,017 - BT4763 0.66 -2.0 6,249,019 - BT4763 0.67 -3.4 6,249,181 + -0.0 6,249,192 + +1.1 6,249,208 + -1.1 6,249,209 - -0.6 6,249,262 - -0.7 6,249,630 + BT4764 0.47 +1.0 6,249,678 + BT4764 0.53 -0.9 6,249,679 - BT4764 0.53 -1.4 6,249,905 + BT4764 0.77 +0.9 6,250,180 - -1.0
Or see this region's nucleotide sequence