Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4462 and BT4463 are separated by 25 nucleotides BT4463 and BT4464 overlap by 4 nucleotides BT4464 and BT4465 are separated by 190 nucleotides
BT4462: BT4462 - conserved hypothetical protein (NCBI ptt file), at 5,872,538 to 5,873,191
BT4462
BT4463: BT4463 - phosphatidate cytidylyltransferase (NCBI ptt file), at 5,873,217 to 5,874,173
BT4463
BT4464: BT4464 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase-related protein (NCBI ptt file), at 5,874,170 to 5,874,823
BT4464
BT4465: BT4465 - conserved hypothetical protein, putative outer membrane protein (NCBI ptt file), at 5,875,014 to 5,876,879
BT4465
Position (kb)
5874
5875 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5873.221 kb on + strand at 5873.231 kb on - strand at 5873.313 kb on - strand, within BT4463 at 5873.313 kb on - strand, within BT4463 at 5873.313 kb on - strand, within BT4463 at 5873.313 kb on - strand, within BT4463 at 5873.362 kb on - strand, within BT4463 at 5873.449 kb on + strand, within BT4463 at 5873.449 kb on + strand, within BT4463 at 5873.455 kb on - strand, within BT4463 at 5873.494 kb on + strand, within BT4463 at 5873.748 kb on + strand, within BT4463 at 5873.749 kb on - strand, within BT4463 at 5873.766 kb on + strand, within BT4463 at 5873.777 kb on + strand, within BT4463 at 5873.778 kb on - strand, within BT4463 at 5873.778 kb on - strand, within BT4463 at 5873.793 kb on + strand, within BT4463 at 5873.800 kb on + strand, within BT4463 at 5873.800 kb on + strand, within BT4463 at 5873.821 kb on + strand, within BT4463 at 5873.831 kb on - strand, within BT4463 at 5874.000 kb on - strand, within BT4463 at 5874.038 kb on + strand, within BT4463 at 5874.039 kb on - strand, within BT4463 at 5874.039 kb on - strand, within BT4463 at 5874.117 kb on + strand at 5874.117 kb on + strand at 5874.118 kb on - strand at 5874.127 kb on - strand at 5874.144 kb on - strand at 5874.150 kb on + strand at 5874.151 kb on - strand at 5874.163 kb on - strand at 5874.216 kb on - strand at 5874.249 kb on - strand, within BT4464 at 5874.251 kb on - strand, within BT4464 at 5874.256 kb on + strand, within BT4464 at 5874.257 kb on - strand, within BT4464 at 5874.384 kb on + strand, within BT4464 at 5874.471 kb on + strand, within BT4464 at 5874.487 kb on + strand, within BT4464 at 5874.528 kb on - strand, within BT4464 at 5874.586 kb on + strand, within BT4464 at 5874.587 kb on - strand, within BT4464 at 5874.590 kb on + strand, within BT4464 at 5874.591 kb on - strand, within BT4464 at 5874.659 kb on - strand, within BT4464 at 5874.659 kb on - strand, within BT4464 at 5874.659 kb on - strand, within BT4464 at 5874.701 kb on + strand, within BT4464 at 5874.761 kb on - strand at 5874.850 kb on + strand at 5874.851 kb on - strand at 5874.853 kb on - strand at 5874.969 kb on + strand at 5875.000 kb on - strand at 5875.013 kb on + strand at 5875.013 kb on + strand at 5875.058 kb on - strand at 5875.065 kb on - strand at 5875.067 kb on + strand at 5875.067 kb on + strand at 5875.080 kb on - strand at 5875.210 kb on - strand, within BT4465 at 5875.242 kb on - strand, within BT4465 at 5875.251 kb on + strand, within BT4465 at 5875.303 kb on + strand, within BT4465 at 5875.327 kb on + strand, within BT4465 at 5875.328 kb on - strand, within BT4465 at 5875.445 kb on - strand, within BT4465 at 5875.492 kb on + strand, within BT4465 at 5875.543 kb on - strand, within BT4465 at 5875.548 kb on - strand, within BT4465 at 5875.550 kb on + strand, within BT4465 at 5875.551 kb on - strand, within BT4465 at 5875.559 kb on + strand, within BT4465 at 5875.669 kb on + strand, within BT4465 at 5875.670 kb on - strand, within BT4465 at 5875.792 kb on + strand, within BT4465 at 5875.802 kb on + strand, within BT4465 at 5875.802 kb on + strand, within BT4465 at 5875.802 kb on + strand, within BT4465
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 5,873,221 + -0.4 5,873,231 - -1.1 5,873,313 - BT4463 0.10 -1.9 5,873,313 - BT4463 0.10 -1.0 5,873,313 - BT4463 0.10 -1.2 5,873,313 - BT4463 0.10 -2.4 5,873,362 - BT4463 0.15 -1.1 5,873,449 + BT4463 0.24 -1.9 5,873,449 + BT4463 0.24 -1.0 5,873,455 - BT4463 0.25 -0.7 5,873,494 + BT4463 0.29 -0.1 5,873,748 + BT4463 0.55 +1.2 5,873,749 - BT4463 0.56 -1.9 5,873,766 + BT4463 0.57 -0.7 5,873,777 + BT4463 0.59 -2.1 5,873,778 - BT4463 0.59 -1.7 5,873,778 - BT4463 0.59 +0.2 5,873,793 + BT4463 0.60 -1.5 5,873,800 + BT4463 0.61 -1.1 5,873,800 + BT4463 0.61 -0.3 5,873,821 + BT4463 0.63 -2.0 5,873,831 - BT4463 0.64 +0.9 5,874,000 - BT4463 0.82 -1.8 5,874,038 + BT4463 0.86 -0.3 5,874,039 - BT4463 0.86 -0.1 5,874,039 - BT4463 0.86 -0.4 5,874,117 + -2.5 5,874,117 + -1.1 5,874,118 - -1.5 5,874,127 - -0.4 5,874,144 - -0.9 5,874,150 + -1.7 5,874,151 - -1.0 5,874,163 - -0.4 5,874,216 - -0.2 5,874,249 - BT4464 0.12 -0.1 5,874,251 - BT4464 0.12 -1.1 5,874,256 + BT4464 0.13 -0.1 5,874,257 - BT4464 0.13 -1.7 5,874,384 + BT4464 0.33 -3.0 5,874,471 + BT4464 0.46 -1.1 5,874,487 + BT4464 0.48 -3.2 5,874,528 - BT4464 0.55 -1.7 5,874,586 + BT4464 0.64 -0.8 5,874,587 - BT4464 0.64 -1.1 5,874,590 + BT4464 0.64 -1.1 5,874,591 - BT4464 0.64 -1.1 5,874,659 - BT4464 0.75 -1.1 5,874,659 - BT4464 0.75 +0.9 5,874,659 - BT4464 0.75 +0.1 5,874,701 + BT4464 0.81 -2.9 5,874,761 - -0.5 5,874,850 + -1.1 5,874,851 - -1.4 5,874,853 - +0.3 5,874,969 + -2.3 5,875,000 - -1.1 5,875,013 + -2.2 5,875,013 + -2.7 5,875,058 - +1.2 5,875,065 - -2.3 5,875,067 + -0.1 5,875,067 + -0.7 5,875,080 - -2.1 5,875,210 - BT4465 0.11 -0.7 5,875,242 - BT4465 0.12 +0.4 5,875,251 + BT4465 0.13 -1.5 5,875,303 + BT4465 0.15 -0.4 5,875,327 + BT4465 0.17 -2.5 5,875,328 - BT4465 0.17 +0.9 5,875,445 - BT4465 0.23 -0.9 5,875,492 + BT4465 0.26 -1.1 5,875,543 - BT4465 0.28 -1.8 5,875,548 - BT4465 0.29 -1.0 5,875,550 + BT4465 0.29 -0.0 5,875,551 - BT4465 0.29 -1.1 5,875,559 + BT4465 0.29 -2.2 5,875,669 + BT4465 0.35 -0.1 5,875,670 - BT4465 0.35 -0.9 5,875,792 + BT4465 0.42 -2.5 5,875,802 + BT4465 0.42 -2.1 5,875,802 + BT4465 0.42 +0.0 5,875,802 + BT4465 0.42 +0.9
Or see this region's nucleotide sequence