Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4426 and BT4427 overlap by 1 nucleotides BT4427 and BT4428 are separated by 36 nucleotides BT4428 and BT4429 are separated by 40 nucleotides
BT4426: BT4426 - conserved hypothetical protein (NCBI ptt file), at 5,828,964 to 5,831,498
BT4426
BT4427: BT4427 - putative surface layer protein, with cytochrome c domain (NCBI ptt file), at 5,831,498 to 5,833,297
BT4427
BT4428: BT4428 - hypothetical protein (NCBI ptt file), at 5,833,334 to 5,834,224
BT4428
BT4429: BT4429 - putative pteridine-dependent dioxygenase (NCBI ptt file), at 5,834,265 to 5,835,410
BT4429
Position (kb) 5831
5832
5833
5834 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5830.513 kb on - strand, within BT4426 at 5830.513 kb on - strand, within BT4426 at 5830.721 kb on - strand, within BT4426 at 5830.721 kb on - strand, within BT4426 at 5831.109 kb on + strand, within BT4426 at 5831.227 kb on - strand, within BT4426 at 5831.249 kb on + strand at 5831.400 kb on + strand at 5831.464 kb on - strand at 5831.514 kb on + strand at 5831.525 kb on - strand at 5831.534 kb on - strand at 5831.774 kb on + strand, within BT4427 at 5831.827 kb on + strand, within BT4427 at 5831.840 kb on - strand, within BT4427 at 5831.846 kb on + strand at 5832.041 kb on - strand, within BT4427 at 5832.156 kb on - strand, within BT4427 at 5832.344 kb on - strand, within BT4427 at 5832.344 kb on - strand, within BT4427 at 5832.358 kb on - strand, within BT4427 at 5832.388 kb on + strand, within BT4427 at 5832.389 kb on - strand, within BT4427 at 5832.389 kb on - strand, within BT4427 at 5832.496 kb on - strand, within BT4427 at 5832.496 kb on - strand, within BT4427 at 5832.496 kb on - strand, within BT4427 at 5832.497 kb on + strand, within BT4427 at 5832.498 kb on - strand, within BT4427 at 5832.498 kb on - strand, within BT4427 at 5832.585 kb on - strand, within BT4427 at 5832.604 kb on + strand, within BT4427 at 5832.734 kb on - strand, within BT4427 at 5832.905 kb on + strand, within BT4427 at 5832.906 kb on - strand, within BT4427 at 5832.936 kb on + strand, within BT4427 at 5832.939 kb on - strand, within BT4427 at 5833.004 kb on - strand, within BT4427 at 5833.077 kb on - strand, within BT4427 at 5833.079 kb on + strand, within BT4427 at 5833.082 kb on - strand, within BT4427 at 5833.172 kb on - strand at 5833.318 kb on - strand at 5833.633 kb on + strand, within BT4428 at 5833.637 kb on - strand, within BT4428 at 5833.637 kb on - strand, within BT4428 at 5834.062 kb on + strand, within BT4428 at 5834.062 kb on + strand, within BT4428 at 5834.100 kb on + strand, within BT4428 at 5834.182 kb on - strand at 5834.182 kb on - strand at 5834.182 kb on - strand at 5834.182 kb on - strand at 5834.186 kb on - strand at 5834.265 kb on - strand at 5834.272 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 5,830,513 - BT4426 0.61 -0.7 5,830,513 - BT4426 0.61 -0.1 5,830,721 - BT4426 0.69 -3.9 5,830,721 - BT4426 0.69 +0.3 5,831,109 + BT4426 0.85 +0.9 5,831,227 - BT4426 0.89 -0.7 5,831,249 + -2.5 5,831,400 + -0.6 5,831,464 - -0.7 5,831,514 + +0.5 5,831,525 - -0.3 5,831,534 - -0.7 5,831,774 + BT4427 0.15 -0.1 5,831,827 + BT4427 0.18 -1.5 5,831,840 - BT4427 0.19 -0.8 5,831,846 + -0.7 5,832,041 - BT4427 0.30 -2.6 5,832,156 - BT4427 0.37 -3.5 5,832,344 - BT4427 0.47 +0.0 5,832,344 - BT4427 0.47 -2.0 5,832,358 - BT4427 0.48 -1.5 5,832,388 + BT4427 0.49 +0.1 5,832,389 - BT4427 0.49 -3.1 5,832,389 - BT4427 0.49 -0.5 5,832,496 - BT4427 0.55 -1.1 5,832,496 - BT4427 0.55 +0.9 5,832,496 - BT4427 0.55 +0.9 5,832,497 + BT4427 0.56 -2.5 5,832,498 - BT4427 0.56 -1.0 5,832,498 - BT4427 0.56 -1.8 5,832,585 - BT4427 0.60 -2.0 5,832,604 + BT4427 0.61 -2.5 5,832,734 - BT4427 0.69 -0.6 5,832,905 + BT4427 0.78 -1.1 5,832,906 - BT4427 0.78 -3.7 5,832,936 + BT4427 0.80 -2.5 5,832,939 - BT4427 0.80 -2.0 5,833,004 - BT4427 0.84 +0.2 5,833,077 - BT4427 0.88 -0.7 5,833,079 + BT4427 0.88 +0.9 5,833,082 - BT4427 0.88 -4.1 5,833,172 - -0.1 5,833,318 - -0.7 5,833,633 + BT4428 0.34 -0.1 5,833,637 - BT4428 0.34 -2.3 5,833,637 - BT4428 0.34 -2.0 5,834,062 + BT4428 0.82 -1.7 5,834,062 + BT4428 0.82 +1.9 5,834,100 + BT4428 0.86 -0.1 5,834,182 - -0.6 5,834,182 - +0.3 5,834,182 - -2.6 5,834,182 - -0.1 5,834,186 - -3.5 5,834,265 - -2.7 5,834,272 - -0.7
Or see this region's nucleotide sequence