Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4031 and BT4032 are separated by 394 nucleotides BT4032 and BT4033 are separated by 226 nucleotides BT4033 and BT4034 are separated by 272 nucleotides
BT4031: BT4031 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 5,243,999 to 5,244,502
BT4031
BT4032: BT4032 - conserved hypothetical protein, putative non-specific DNA-binding protein (NCBI ptt file), at 5,244,897 to 5,245,373
BT4032
BT4033: BT4033 - hypothetical protein (NCBI ptt file), at 5,245,600 to 5,246,088
BT4033
BT4034: BT4034 - hypothetical protein (NCBI ptt file), at 5,246,361 to 5,246,783
BT4034
Position (kb) 5244
5245
5246 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5243.948 kb on + strand at 5243.974 kb on + strand at 5244.131 kb on + strand, within BT4031 at 5244.131 kb on + strand, within BT4031 at 5244.132 kb on - strand, within BT4031 at 5244.172 kb on + strand, within BT4031 at 5244.271 kb on + strand, within BT4031 at 5244.487 kb on - strand at 5244.497 kb on - strand at 5244.498 kb on + strand at 5244.500 kb on + strand at 5244.500 kb on + strand at 5244.500 kb on + strand at 5244.500 kb on + strand at 5244.501 kb on - strand at 5244.525 kb on - strand at 5244.577 kb on + strand at 5244.778 kb on + strand at 5244.782 kb on + strand at 5244.803 kb on - strand at 5244.803 kb on - strand at 5244.803 kb on - strand at 5244.810 kb on + strand at 5244.810 kb on + strand at 5244.814 kb on + strand at 5244.846 kb on + strand at 5244.847 kb on - strand at 5244.850 kb on + strand at 5244.875 kb on + strand at 5244.892 kb on + strand at 5244.963 kb on + strand, within BT4032 at 5244.964 kb on - strand, within BT4032 at 5245.037 kb on - strand, within BT4032 at 5245.113 kb on - strand, within BT4032 at 5245.218 kb on + strand, within BT4032 at 5245.218 kb on + strand, within BT4032 at 5245.219 kb on - strand, within BT4032 at 5245.223 kb on - strand, within BT4032 at 5245.223 kb on - strand, within BT4032 at 5245.257 kb on + strand, within BT4032 at 5245.257 kb on + strand, within BT4032 at 5245.258 kb on - strand at 5245.391 kb on + strand at 5245.491 kb on - strand at 5245.576 kb on + strand at 5245.577 kb on - strand at 5245.579 kb on - strand at 5245.579 kb on - strand at 5245.579 kb on - strand at 5245.623 kb on + strand at 5245.624 kb on - strand at 5245.624 kb on - strand at 5245.654 kb on - strand, within BT4033 at 5245.658 kb on - strand, within BT4033 at 5245.687 kb on - strand, within BT4033 at 5245.782 kb on + strand, within BT4033 at 5245.835 kb on + strand, within BT4033 at 5245.860 kb on - strand, within BT4033 at 5246.075 kb on + strand at 5246.118 kb on + strand at 5246.180 kb on - strand at 5246.218 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 5,243,948 + -3.3 5,243,974 + -0.0 5,244,131 + BT4031 0.26 -1.8 5,244,131 + BT4031 0.26 +1.0 5,244,132 - BT4031 0.26 -2.0 5,244,172 + BT4031 0.34 -0.5 5,244,271 + BT4031 0.54 -0.1 5,244,487 - +0.4 5,244,497 - -1.8 5,244,498 + -1.3 5,244,500 + -0.6 5,244,500 + -0.0 5,244,500 + +0.4 5,244,500 + -1.8 5,244,501 - -1.8 5,244,525 - +0.5 5,244,577 + -0.8 5,244,778 + -0.8 5,244,782 + -1.4 5,244,803 - -1.4 5,244,803 - -1.8 5,244,803 - -2.2 5,244,810 + -2.6 5,244,810 + -0.3 5,244,814 + -1.0 5,244,846 + -2.4 5,244,847 - -0.0 5,244,850 + -2.7 5,244,875 + -1.4 5,244,892 + -3.3 5,244,963 + BT4032 0.14 -1.4 5,244,964 - BT4032 0.14 -1.5 5,245,037 - BT4032 0.29 -1.8 5,245,113 - BT4032 0.45 -1.0 5,245,218 + BT4032 0.67 -0.6 5,245,218 + BT4032 0.67 -2.0 5,245,219 - BT4032 0.68 -2.0 5,245,223 - BT4032 0.68 -1.8 5,245,223 - BT4032 0.68 -1.6 5,245,257 + BT4032 0.75 +0.4 5,245,257 + BT4032 0.75 -2.5 5,245,258 - -1.4 5,245,391 + -2.5 5,245,491 - -1.6 5,245,576 + -1.4 5,245,577 - -1.4 5,245,579 - +0.5 5,245,579 - +0.4 5,245,579 - -1.6 5,245,623 + +0.4 5,245,624 - +0.6 5,245,624 - -1.0 5,245,654 - BT4033 0.11 -0.6 5,245,658 - BT4033 0.12 -2.7 5,245,687 - BT4033 0.18 -3.0 5,245,782 + BT4033 0.37 +0.5 5,245,835 + BT4033 0.48 -1.5 5,245,860 - BT4033 0.53 -3.2 5,246,075 + -0.8 5,246,118 + -1.0 5,246,180 - -2.8 5,246,218 + +0.4
Or see this region's nucleotide sequence