Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3755 and BT3756 are separated by 7 nucleotides BT3756 and BT3757 are separated by 39 nucleotides BT3757 and BT3758 are separated by 135 nucleotides
BT3755: BT3755 - acetyl-coenzyme A synthetase (NCBI ptt file), at 4,875,308 to 4,876,963
BT3755
BT3756: BT3756 - transcriptional regulator (NCBI ptt file), at 4,876,971 to 4,877,525
BT3756
BT3757: BT3757 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 4,877,565 to 4,878,338
BT3757
BT3758: BT3758 - N-succinylornithine aminotransferase (EC 2.6.1.81) (from data) , at 4,878,474 to 4,879,595
BT3758
Position (kb)
4876
4877
4878 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4876.041 kb on + strand, within BT3755 at 4876.041 kb on + strand, within BT3755 at 4876.042 kb on - strand, within BT3755 at 4876.042 kb on - strand, within BT3755 at 4876.042 kb on - strand, within BT3755 at 4876.042 kb on - strand, within BT3755 at 4876.045 kb on + strand, within BT3755 at 4876.045 kb on + strand, within BT3755 at 4876.045 kb on + strand, within BT3755 at 4876.046 kb on - strand, within BT3755 at 4876.162 kb on - strand, within BT3755 at 4876.312 kb on + strand, within BT3755 at 4876.312 kb on + strand, within BT3755 at 4876.313 kb on - strand, within BT3755 at 4876.326 kb on - strand, within BT3755 at 4876.333 kb on + strand, within BT3755 at 4876.334 kb on - strand, within BT3755 at 4876.334 kb on - strand, within BT3755 at 4876.387 kb on + strand, within BT3755 at 4876.588 kb on - strand, within BT3755 at 4876.588 kb on - strand, within BT3755 at 4876.595 kb on - strand, within BT3755 at 4876.646 kb on - strand, within BT3755 at 4876.737 kb on + strand, within BT3755 at 4876.856 kb on + strand at 4876.857 kb on - strand at 4876.859 kb on + strand at 4876.896 kb on - strand at 4876.937 kb on - strand at 4876.937 kb on - strand at 4876.937 kb on - strand at 4876.958 kb on + strand at 4876.958 kb on + strand at 4876.959 kb on - strand at 4876.977 kb on - strand at 4877.047 kb on + strand, within BT3756 at 4877.102 kb on + strand, within BT3756 at 4877.149 kb on + strand at 4877.355 kb on - strand, within BT3756 at 4877.362 kb on - strand, within BT3756 at 4877.375 kb on + strand, within BT3756 at 4877.375 kb on + strand, within BT3756 at 4877.376 kb on - strand, within BT3756 at 4877.417 kb on - strand, within BT3756 at 4877.430 kb on + strand, within BT3756 at 4877.463 kb on + strand, within BT3756 at 4877.463 kb on + strand, within BT3756 at 4877.473 kb on - strand at 4877.510 kb on + strand at 4877.566 kb on + strand at 4877.643 kb on - strand, within BT3757 at 4877.659 kb on + strand, within BT3757 at 4877.706 kb on + strand, within BT3757 at 4877.737 kb on - strand, within BT3757 at 4877.737 kb on - strand, within BT3757 at 4877.803 kb on + strand, within BT3757 at 4877.816 kb on - strand, within BT3757 at 4877.909 kb on + strand, within BT3757 at 4878.244 kb on - strand, within BT3757 at 4878.244 kb on - strand, within BT3757 at 4878.251 kb on + strand, within BT3757 at 4878.251 kb on + strand, within BT3757 at 4878.296 kb on + strand at 4878.326 kb on + strand at 4878.330 kb on + strand at 4878.358 kb on + strand at 4878.359 kb on - strand at 4878.365 kb on - strand at 4878.383 kb on - strand at 4878.414 kb on - strand at 4878.458 kb on - strand at 4878.476 kb on - strand at 4878.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 4,876,041 + BT3755 0.44 -1.7 4,876,041 + BT3755 0.44 +0.1 4,876,042 - BT3755 0.44 -0.1 4,876,042 - BT3755 0.44 -0.0 4,876,042 - BT3755 0.44 -0.1 4,876,042 - BT3755 0.44 -0.5 4,876,045 + BT3755 0.45 -1.8 4,876,045 + BT3755 0.45 -2.6 4,876,045 + BT3755 0.45 +0.6 4,876,046 - BT3755 0.45 -1.3 4,876,162 - BT3755 0.52 -2.1 4,876,312 + BT3755 0.61 +0.1 4,876,312 + BT3755 0.61 -0.5 4,876,313 - BT3755 0.61 +0.8 4,876,326 - BT3755 0.61 -2.4 4,876,333 + BT3755 0.62 -0.5 4,876,334 - BT3755 0.62 +0.4 4,876,334 - BT3755 0.62 -0.1 4,876,387 + BT3755 0.65 -1.5 4,876,588 - BT3755 0.77 -3.4 4,876,588 - BT3755 0.77 -1.0 4,876,595 - BT3755 0.78 +1.0 4,876,646 - BT3755 0.81 +0.0 4,876,737 + BT3755 0.86 -2.0 4,876,856 + +0.0 4,876,857 - -2.0 4,876,859 + -0.9 4,876,896 - -1.5 4,876,937 - -2.0 4,876,937 - -2.0 4,876,937 - -1.1 4,876,958 + -2.3 4,876,958 + -1.8 4,876,959 - -1.5 4,876,977 - -2.7 4,877,047 + BT3756 0.14 -3.8 4,877,102 + BT3756 0.24 -0.4 4,877,149 + +1.0 4,877,355 - BT3756 0.69 -1.0 4,877,362 - BT3756 0.70 -2.0 4,877,375 + BT3756 0.73 -2.5 4,877,375 + BT3756 0.73 -2.7 4,877,376 - BT3756 0.73 -1.1 4,877,417 - BT3756 0.80 -3.6 4,877,430 + BT3756 0.83 -0.1 4,877,463 + BT3756 0.89 -2.1 4,877,463 + BT3756 0.89 -2.0 4,877,473 - -2.8 4,877,510 + -0.7 4,877,566 + -2.1 4,877,643 - BT3757 0.10 -0.2 4,877,659 + BT3757 0.12 -1.0 4,877,706 + BT3757 0.18 -1.9 4,877,737 - BT3757 0.22 +0.6 4,877,737 - BT3757 0.22 -2.7 4,877,803 + BT3757 0.31 -3.9 4,877,816 - BT3757 0.32 -1.5 4,877,909 + BT3757 0.44 -3.0 4,878,244 - BT3757 0.88 -3.0 4,878,244 - BT3757 0.88 -0.4 4,878,251 + BT3757 0.89 -1.2 4,878,251 + BT3757 0.89 -1.0 4,878,296 + -0.0 4,878,326 + -1.1 4,878,330 + -2.4 4,878,358 + -3.0 4,878,359 - -1.3 4,878,365 - +0.2 4,878,383 - +0.2 4,878,414 - -1.5 4,878,458 - -2.1 4,878,476 - -1.8 4,878,481 - +0.0
Or see this region's nucleotide sequence