Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3550 and BT3551 are separated by 131 nucleotides BT3551 and BT3552 are separated by 88 nucleotides
BT3550: BT3550 - putative long-chain-fatty-acid--CoA ligase (NCBI ptt file), at 4,589,767 to 4,591,572
BT3550
BT3551: BT3551 - putative zinc protease (NCBI ptt file), at 4,591,704 to 4,594,796
BT3551
BT3552: BT3552 - peptide chain release factor RF-2 (NCBI ptt file), at 4,594,885 to 4,595,844
BT3552
Position (kb)
4591
4592
4593
4594
4595 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4590.775 kb on + strand, within BT3550 at 4590.934 kb on - strand, within BT3550 at 4590.944 kb on + strand, within BT3550 at 4590.949 kb on + strand, within BT3550 at 4591.049 kb on - strand, within BT3550 at 4591.051 kb on + strand, within BT3550 at 4591.051 kb on + strand, within BT3550 at 4591.189 kb on + strand, within BT3550 at 4591.190 kb on - strand, within BT3550 at 4591.227 kb on - strand, within BT3550 at 4591.234 kb on + strand, within BT3550 at 4591.234 kb on + strand, within BT3550 at 4591.498 kb on - strand at 4591.561 kb on + strand at 4591.674 kb on + strand at 4591.675 kb on - strand at 4591.699 kb on - strand at 4591.764 kb on - strand at 4591.801 kb on + strand at 4591.808 kb on - strand at 4591.862 kb on + strand at 4591.862 kb on + strand at 4591.930 kb on + strand at 4591.983 kb on + strand at 4592.051 kb on + strand, within BT3551 at 4592.097 kb on - strand, within BT3551 at 4592.097 kb on - strand, within BT3551 at 4592.097 kb on - strand, within BT3551 at 4592.101 kb on - strand, within BT3551 at 4592.105 kb on + strand, within BT3551 at 4592.105 kb on + strand, within BT3551 at 4592.107 kb on + strand, within BT3551 at 4592.108 kb on - strand, within BT3551 at 4592.113 kb on + strand, within BT3551 at 4592.114 kb on - strand, within BT3551 at 4592.137 kb on - strand, within BT3551 at 4592.228 kb on - strand, within BT3551 at 4592.229 kb on + strand, within BT3551 at 4592.248 kb on - strand, within BT3551 at 4592.291 kb on + strand, within BT3551 at 4592.292 kb on - strand, within BT3551 at 4592.293 kb on + strand, within BT3551 at 4592.294 kb on - strand, within BT3551 at 4592.294 kb on - strand, within BT3551 at 4592.294 kb on - strand, within BT3551 at 4592.375 kb on + strand, within BT3551 at 4592.404 kb on + strand, within BT3551 at 4592.452 kb on + strand, within BT3551 at 4592.452 kb on + strand, within BT3551 at 4592.453 kb on - strand, within BT3551 at 4592.453 kb on - strand, within BT3551 at 4592.471 kb on - strand, within BT3551 at 4592.569 kb on + strand, within BT3551 at 4592.570 kb on - strand, within BT3551 at 4592.578 kb on + strand, within BT3551 at 4592.640 kb on + strand, within BT3551 at 4592.694 kb on + strand, within BT3551 at 4592.707 kb on - strand, within BT3551 at 4592.755 kb on + strand, within BT3551 at 4592.783 kb on - strand, within BT3551 at 4592.860 kb on + strand, within BT3551 at 4592.897 kb on + strand, within BT3551 at 4592.897 kb on + strand, within BT3551 at 4592.915 kb on - strand, within BT3551 at 4592.953 kb on + strand, within BT3551 at 4592.953 kb on + strand, within BT3551 at 4593.056 kb on + strand, within BT3551 at 4593.057 kb on - strand, within BT3551 at 4593.098 kb on + strand, within BT3551 at 4593.152 kb on - strand, within BT3551 at 4593.152 kb on - strand, within BT3551 at 4593.181 kb on + strand, within BT3551 at 4593.222 kb on - strand, within BT3551 at 4593.332 kb on - strand, within BT3551 at 4593.350 kb on + strand, within BT3551 at 4593.505 kb on + strand, within BT3551 at 4593.505 kb on + strand, within BT3551 at 4593.506 kb on - strand, within BT3551 at 4593.506 kb on - strand, within BT3551 at 4593.568 kb on + strand, within BT3551 at 4593.568 kb on + strand, within BT3551 at 4593.569 kb on - strand, within BT3551 at 4593.571 kb on - strand, within BT3551 at 4593.571 kb on - strand, within BT3551 at 4593.573 kb on + strand, within BT3551 at 4593.663 kb on + strand, within BT3551 at 4593.663 kb on + strand, within BT3551 at 4593.804 kb on - strand, within BT3551 at 4593.819 kb on - strand, within BT3551 at 4593.845 kb on - strand, within BT3551 at 4593.997 kb on + strand, within BT3551 at 4594.105 kb on + strand, within BT3551 at 4594.119 kb on + strand, within BT3551 at 4594.120 kb on + strand, within BT3551 at 4594.120 kb on + strand, within BT3551 at 4594.130 kb on - strand, within BT3551 at 4594.207 kb on + strand, within BT3551 at 4594.232 kb on - strand, within BT3551 at 4594.321 kb on + strand, within BT3551 at 4594.322 kb on - strand, within BT3551 at 4594.337 kb on - strand, within BT3551 at 4594.538 kb on - strand at 4594.541 kb on + strand at 4594.541 kb on + strand at 4594.543 kb on + strand at 4594.543 kb on + strand at 4594.543 kb on + strand at 4594.544 kb on - strand at 4594.594 kb on - strand at 4594.623 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 4,590,775 + BT3550 0.56 -1.6 4,590,934 - BT3550 0.65 -1.0 4,590,944 + BT3550 0.65 -1.8 4,590,949 + BT3550 0.65 +0.0 4,591,049 - BT3550 0.71 -2.5 4,591,051 + BT3550 0.71 -1.3 4,591,051 + BT3550 0.71 -1.6 4,591,189 + BT3550 0.79 -0.8 4,591,190 - BT3550 0.79 +1.0 4,591,227 - BT3550 0.81 -2.5 4,591,234 + BT3550 0.81 -2.4 4,591,234 + BT3550 0.81 -2.9 4,591,498 - -0.8 4,591,561 + -3.4 4,591,674 + -3.9 4,591,675 - -1.2 4,591,699 - -0.1 4,591,764 - +0.2 4,591,801 + -1.8 4,591,808 - -1.3 4,591,862 + -1.8 4,591,862 + +0.0 4,591,930 + -0.1 4,591,983 + +0.2 4,592,051 + BT3551 0.11 -0.2 4,592,097 - BT3551 0.13 -2.7 4,592,097 - BT3551 0.13 -1.4 4,592,097 - BT3551 0.13 -0.2 4,592,101 - BT3551 0.13 -1.2 4,592,105 + BT3551 0.13 -2.7 4,592,105 + BT3551 0.13 -1.6 4,592,107 + BT3551 0.13 -0.6 4,592,108 - BT3551 0.13 +0.4 4,592,113 + BT3551 0.13 -3.3 4,592,114 - BT3551 0.13 -2.8 4,592,137 - BT3551 0.14 -2.4 4,592,228 - BT3551 0.17 -1.0 4,592,229 + BT3551 0.17 -2.1 4,592,248 - BT3551 0.18 +0.9 4,592,291 + BT3551 0.19 -2.1 4,592,292 - BT3551 0.19 -1.2 4,592,293 + BT3551 0.19 -1.6 4,592,294 - BT3551 0.19 +0.2 4,592,294 - BT3551 0.19 -1.0 4,592,294 - BT3551 0.19 -1.3 4,592,375 + BT3551 0.22 +0.1 4,592,404 + BT3551 0.23 -1.6 4,592,452 + BT3551 0.24 -2.2 4,592,452 + BT3551 0.24 -4.0 4,592,453 - BT3551 0.24 -0.1 4,592,453 - BT3551 0.24 -1.0 4,592,471 - BT3551 0.25 -0.3 4,592,569 + BT3551 0.28 -1.0 4,592,570 - BT3551 0.28 -2.2 4,592,578 + BT3551 0.28 +0.1 4,592,640 + BT3551 0.30 +0.3 4,592,694 + BT3551 0.32 -1.5 4,592,707 - BT3551 0.32 -1.9 4,592,755 + BT3551 0.34 -0.2 4,592,783 - BT3551 0.35 -2.1 4,592,860 + BT3551 0.37 -0.6 4,592,897 + BT3551 0.39 -1.8 4,592,897 + BT3551 0.39 -1.9 4,592,915 - BT3551 0.39 -2.4 4,592,953 + BT3551 0.40 +0.0 4,592,953 + BT3551 0.40 -0.6 4,593,056 + BT3551 0.44 -1.3 4,593,057 - BT3551 0.44 +0.0 4,593,098 + BT3551 0.45 -1.3 4,593,152 - BT3551 0.47 -0.8 4,593,152 - BT3551 0.47 -0.2 4,593,181 + BT3551 0.48 -0.1 4,593,222 - BT3551 0.49 -1.3 4,593,332 - BT3551 0.53 -0.3 4,593,350 + BT3551 0.53 -2.6 4,593,505 + BT3551 0.58 -2.1 4,593,505 + BT3551 0.58 -3.4 4,593,506 - BT3551 0.58 -2.8 4,593,506 - BT3551 0.58 +1.3 4,593,568 + BT3551 0.60 -1.3 4,593,568 + BT3551 0.60 -2.1 4,593,569 - BT3551 0.60 +0.0 4,593,571 - BT3551 0.60 -3.0 4,593,571 - BT3551 0.60 -0.7 4,593,573 + BT3551 0.60 -1.0 4,593,663 + BT3551 0.63 -2.3 4,593,663 + BT3551 0.63 -1.5 4,593,804 - BT3551 0.68 -2.7 4,593,819 - BT3551 0.68 -3.2 4,593,845 - BT3551 0.69 -2.4 4,593,997 + BT3551 0.74 +0.1 4,594,105 + BT3551 0.78 -0.3 4,594,119 + BT3551 0.78 -2.1 4,594,120 + BT3551 0.78 -2.6 4,594,120 + BT3551 0.78 -2.0 4,594,130 - BT3551 0.78 -1.3 4,594,207 + BT3551 0.81 -2.3 4,594,232 - BT3551 0.82 -0.0 4,594,321 + BT3551 0.85 -0.8 4,594,322 - BT3551 0.85 -1.8 4,594,337 - BT3551 0.85 -2.6 4,594,538 - -2.0 4,594,541 + +0.7 4,594,541 + -1.0 4,594,543 + -1.1 4,594,543 + -0.5 4,594,543 + -0.1 4,594,544 - -1.3 4,594,594 - -1.8 4,594,623 - -3.9
Or see this region's nucleotide sequence