Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2659 and BT2660 are separated by 96 nucleotides BT2660 and BT2661 are separated by 381 nucleotides BT2661 and tRNA-Pro are separated by 220 nucleotides
BT2659: BT2659 - conserved hypothetical protein (NCBI ptt file), at 3,314,081 to 3,315,421
BT2659
BT2660: BT2660 - hypothetical protein (NCBI ptt file), at 3,315,518 to 3,316,108
BT2660
BT2661: BT2661 - hypothetical protein (NCBI ptt file), at 3,316,490 to 3,316,684
BT2661
BT_t41: tRNA-Pro - tRNA-Pro (RefSeq), at 3,316,905 to 3,316,980
tRNA-Pro
Position (kb)
3315
3316
3317 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3314.569 kb on + strand, within BT2659 at 3314.607 kb on - strand, within BT2659 at 3314.617 kb on + strand, within BT2659 at 3314.618 kb on - strand, within BT2659 at 3314.730 kb on - strand, within BT2659 at 3314.733 kb on + strand, within BT2659 at 3314.733 kb on + strand, within BT2659 at 3314.733 kb on + strand, within BT2659 at 3314.828 kb on - strand, within BT2659 at 3314.828 kb on - strand, within BT2659 at 3314.863 kb on + strand, within BT2659 at 3314.864 kb on - strand, within BT2659 at 3314.866 kb on + strand, within BT2659 at 3314.866 kb on + strand, within BT2659 at 3314.866 kb on + strand, within BT2659 at 3314.869 kb on + strand, within BT2659 at 3314.869 kb on + strand, within BT2659 at 3314.869 kb on + strand, within BT2659 at 3314.869 kb on + strand, within BT2659 at 3314.870 kb on - strand, within BT2659 at 3314.882 kb on + strand, within BT2659 at 3315.136 kb on - strand, within BT2659 at 3315.148 kb on + strand, within BT2659 at 3315.149 kb on - strand, within BT2659 at 3315.167 kb on + strand, within BT2659 at 3315.263 kb on + strand, within BT2659 at 3315.264 kb on - strand, within BT2659 at 3315.278 kb on - strand, within BT2659 at 3315.295 kb on + strand at 3315.295 kb on + strand at 3315.401 kb on + strand at 3315.422 kb on + strand at 3315.455 kb on + strand at 3315.469 kb on + strand at 3315.469 kb on + strand at 3315.469 kb on + strand at 3315.470 kb on - strand at 3315.494 kb on - strand at 3315.564 kb on + strand at 3315.615 kb on + strand, within BT2660 at 3315.700 kb on - strand, within BT2660 at 3315.700 kb on - strand, within BT2660 at 3315.755 kb on + strand, within BT2660 at 3315.802 kb on + strand, within BT2660 at 3315.802 kb on + strand, within BT2660 at 3315.802 kb on + strand, within BT2660 at 3315.804 kb on + strand, within BT2660 at 3315.805 kb on - strand, within BT2660 at 3315.901 kb on + strand, within BT2660 at 3315.930 kb on + strand, within BT2660 at 3315.930 kb on + strand, within BT2660 at 3315.931 kb on - strand, within BT2660 at 3315.934 kb on + strand, within BT2660 at 3316.001 kb on - strand, within BT2660 at 3316.008 kb on + strand, within BT2660 at 3316.008 kb on + strand, within BT2660 at 3316.009 kb on - strand, within BT2660 at 3316.031 kb on + strand, within BT2660 at 3316.128 kb on + strand at 3316.153 kb on + strand at 3316.176 kb on + strand at 3316.321 kb on + strand at 3316.368 kb on - strand at 3316.372 kb on - strand at 3316.507 kb on + strand at 3316.518 kb on + strand, within BT2661 at 3316.529 kb on + strand, within BT2661 at 3316.530 kb on - strand, within BT2661 at 3316.538 kb on + strand, within BT2661 at 3316.571 kb on + strand, within BT2661 at 3316.650 kb on + strand, within BT2661 at 3316.652 kb on + strand, within BT2661 at 3316.664 kb on + strand at 3316.672 kb on + strand at 3316.685 kb on + strand at 3316.717 kb on + strand at 3316.798 kb on + strand at 3316.895 kb on + strand at 3317.038 kb on - strand at 3317.063 kb on + strand at 3317.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 3,314,569 + BT2659 0.36 +0.5 3,314,607 - BT2659 0.39 -0.1 3,314,617 + BT2659 0.40 -1.5 3,314,618 - BT2659 0.40 -2.2 3,314,730 - BT2659 0.48 -2.4 3,314,733 + BT2659 0.49 -1.4 3,314,733 + BT2659 0.49 -0.4 3,314,733 + BT2659 0.49 +0.2 3,314,828 - BT2659 0.56 -0.8 3,314,828 - BT2659 0.56 -0.4 3,314,863 + BT2659 0.58 -1.4 3,314,864 - BT2659 0.58 -0.8 3,314,866 + BT2659 0.59 +0.4 3,314,866 + BT2659 0.59 +0.2 3,314,866 + BT2659 0.59 +0.5 3,314,869 + BT2659 0.59 -2.3 3,314,869 + BT2659 0.59 -0.5 3,314,869 + BT2659 0.59 -2.2 3,314,869 + BT2659 0.59 +0.3 3,314,870 - BT2659 0.59 -2.3 3,314,882 + BT2659 0.60 -2.5 3,315,136 - BT2659 0.79 -1.8 3,315,148 + BT2659 0.80 -0.7 3,315,149 - BT2659 0.80 -0.8 3,315,167 + BT2659 0.81 -2.2 3,315,263 + BT2659 0.88 -2.8 3,315,264 - BT2659 0.88 -1.2 3,315,278 - BT2659 0.89 -0.8 3,315,295 + +0.4 3,315,295 + -1.0 3,315,401 + -0.4 3,315,422 + -2.2 3,315,455 + -0.4 3,315,469 + -0.2 3,315,469 + -0.7 3,315,469 + -2.2 3,315,470 - -2.5 3,315,494 - -0.2 3,315,564 + -0.1 3,315,615 + BT2660 0.16 -1.6 3,315,700 - BT2660 0.31 -1.6 3,315,700 - BT2660 0.31 -1.9 3,315,755 + BT2660 0.40 -1.5 3,315,802 + BT2660 0.48 -2.7 3,315,802 + BT2660 0.48 -4.6 3,315,802 + BT2660 0.48 -0.3 3,315,804 + BT2660 0.48 -1.4 3,315,805 - BT2660 0.49 +0.8 3,315,901 + BT2660 0.65 -1.3 3,315,930 + BT2660 0.70 -3.7 3,315,930 + BT2660 0.70 -2.2 3,315,931 - BT2660 0.70 -1.6 3,315,934 + BT2660 0.70 +0.7 3,316,001 - BT2660 0.82 -1.5 3,316,008 + BT2660 0.83 +0.2 3,316,008 + BT2660 0.83 -1.6 3,316,009 - BT2660 0.83 +1.2 3,316,031 + BT2660 0.87 -1.5 3,316,128 + -0.2 3,316,153 + -1.1 3,316,176 + +0.6 3,316,321 + -0.4 3,316,368 - -1.2 3,316,372 - +0.2 3,316,507 + -1.5 3,316,518 + BT2661 0.14 +1.2 3,316,529 + BT2661 0.20 -1.1 3,316,530 - BT2661 0.21 -0.8 3,316,538 + BT2661 0.25 -2.3 3,316,571 + BT2661 0.42 -2.9 3,316,650 + BT2661 0.82 +0.3 3,316,652 + BT2661 0.83 +0.3 3,316,664 + +0.2 3,316,672 + -0.4 3,316,685 + -0.7 3,316,717 + +1.2 3,316,798 + +0.3 3,316,895 + -1.2 3,317,038 - +0.5 3,317,063 + -0.8 3,317,105 + +0.2
Or see this region's nucleotide sequence