Experiment: BHIS with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0338 and BT0339 are separated by 121 nucleotides BT0339 and BT0340 are separated by 9 nucleotides
BT0338: BT0338 - conserved hypothetical protein (NCBI ptt file), at 403,243 to 405,093
BT0338
BT0339: BT0339 - alpha-glucosidase (NCBI ptt file), at 405,215 to 407,461
BT0339
BT0340: BT0340 - trimethylamine corrinoid protein 2 (TCP 2) (NCBI ptt file), at 407,471 to 409,279
BT0340
Position (kb) 405
406
407
408 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 404.221 kb on - strand, within BT0338 at 404.292 kb on + strand, within BT0338 at 404.370 kb on - strand, within BT0338 at 404.375 kb on + strand, within BT0338 at 404.503 kb on - strand, within BT0338 at 404.582 kb on + strand, within BT0338 at 404.595 kb on + strand, within BT0338 at 404.665 kb on + strand, within BT0338 at 404.834 kb on - strand, within BT0338 at 404.835 kb on + strand, within BT0338 at 404.927 kb on - strand at 405.096 kb on - strand at 405.180 kb on + strand at 405.202 kb on + strand at 405.205 kb on + strand at 405.268 kb on - strand at 405.298 kb on + strand at 405.299 kb on - strand at 405.332 kb on - strand at 405.332 kb on - strand at 405.366 kb on + strand at 405.376 kb on + strand at 405.376 kb on + strand at 405.376 kb on + strand at 405.376 kb on + strand at 405.377 kb on - strand at 405.412 kb on + strand at 405.433 kb on + strand at 405.677 kb on + strand, within BT0339 at 405.906 kb on + strand, within BT0339 at 405.934 kb on - strand, within BT0339 at 405.971 kb on + strand, within BT0339 at 406.176 kb on - strand, within BT0339 at 406.194 kb on + strand, within BT0339 at 406.431 kb on - strand, within BT0339 at 406.433 kb on - strand, within BT0339 at 406.511 kb on - strand, within BT0339 at 406.659 kb on - strand, within BT0339 at 406.883 kb on - strand, within BT0339 at 407.070 kb on + strand, within BT0339 at 407.309 kb on + strand at 407.309 kb on + strand at 407.310 kb on - strand at 407.428 kb on - strand at 407.434 kb on + strand at 407.438 kb on + strand at 407.514 kb on - strand at 407.538 kb on + strand at 407.552 kb on - strand at 407.672 kb on + strand, within BT0340 at 407.673 kb on - strand, within BT0340 at 407.673 kb on - strand, within BT0340 at 407.794 kb on - strand, within BT0340 at 407.813 kb on - strand, within BT0340 at 407.881 kb on + strand, within BT0340 at 407.963 kb on - strand, within BT0340 at 407.963 kb on - strand, within BT0340 at 407.963 kb on - strand, within BT0340 at 408.039 kb on - strand, within BT0340 at 408.177 kb on - strand, within BT0340 at 408.236 kb on + strand, within BT0340 at 408.237 kb on - strand, within BT0340
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chenodeoxycholic 0.25 mg/ml remove 404,221 - BT0338 0.53 -1.7 404,292 + BT0338 0.57 -0.9 404,370 - BT0338 0.61 -0.5 404,375 + BT0338 0.61 -2.0 404,503 - BT0338 0.68 +0.9 404,582 + BT0338 0.72 -1.3 404,595 + BT0338 0.73 +1.0 404,665 + BT0338 0.77 -1.1 404,834 - BT0338 0.86 -0.1 404,835 + BT0338 0.86 +1.3 404,927 - -2.0 405,096 - -2.2 405,180 + -1.5 405,202 + -0.1 405,205 + -1.1 405,268 - -3.1 405,298 + +0.4 405,299 - -0.2 405,332 - -0.7 405,332 - +1.1 405,366 + -0.2 405,376 + -1.5 405,376 + -2.2 405,376 + +0.8 405,376 + -1.2 405,377 - -0.7 405,412 + +0.8 405,433 + -3.9 405,677 + BT0339 0.21 -2.7 405,906 + BT0339 0.31 -1.2 405,934 - BT0339 0.32 -1.2 405,971 + BT0339 0.34 +0.5 406,176 - BT0339 0.43 +1.3 406,194 + BT0339 0.44 +0.8 406,431 - BT0339 0.54 -1.5 406,433 - BT0339 0.54 -1.0 406,511 - BT0339 0.58 +1.5 406,659 - BT0339 0.64 +0.1 406,883 - BT0339 0.74 -0.7 407,070 + BT0339 0.83 +0.4 407,309 + -2.7 407,309 + -0.2 407,310 - -1.2 407,428 - +0.5 407,434 + -0.2 407,438 + -0.2 407,514 - -0.2 407,538 + -2.6 407,552 - -1.7 407,672 + BT0340 0.11 -1.2 407,673 - BT0340 0.11 -0.2 407,673 - BT0340 0.11 -1.2 407,794 - BT0340 0.18 -3.3 407,813 - BT0340 0.19 +0.2 407,881 + BT0340 0.23 -1.5 407,963 - BT0340 0.27 -0.2 407,963 - BT0340 0.27 -1.5 407,963 - BT0340 0.27 -3.2 408,039 - BT0340 0.31 -1.4 408,177 - BT0340 0.39 +0.8 408,236 + BT0340 0.42 -1.2 408,237 - BT0340 0.42 -3.2
Or see this region's nucleotide sequence