Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_818 and Pf1N1B4_819 are separated by 349 nucleotides Pf1N1B4_819 and Pf1N1B4_820 are separated by 331 nucleotides
Pf1N1B4_818 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 934,867 to 936,255
_818
Pf1N1B4_819 - Benzoate transport protein, at 936,605 to 937,810
_819
Pf1N1B4_820 - Glucose-selective porin OprB, at 938,142 to 939,479
_820
Position (kb)
936
937
938 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 935.850 kb on - strand, within Pf1N1B4_818 at 935.913 kb on - strand, within Pf1N1B4_818 at 935.931 kb on - strand, within Pf1N1B4_818 at 935.931 kb on - strand, within Pf1N1B4_818 at 935.931 kb on - strand, within Pf1N1B4_818 at 935.931 kb on - strand, within Pf1N1B4_818 at 935.931 kb on - strand, within Pf1N1B4_818 at 935.957 kb on - strand, within Pf1N1B4_818 at 935.992 kb on - strand, within Pf1N1B4_818 at 935.992 kb on - strand, within Pf1N1B4_818 at 936.028 kb on + strand, within Pf1N1B4_818 at 936.028 kb on + strand, within Pf1N1B4_818 at 936.028 kb on + strand, within Pf1N1B4_818 at 936.029 kb on - strand, within Pf1N1B4_818 at 936.029 kb on - strand, within Pf1N1B4_818 at 936.029 kb on - strand, within Pf1N1B4_818 at 936.029 kb on - strand, within Pf1N1B4_818 at 936.029 kb on - strand, within Pf1N1B4_818 at 936.032 kb on + strand, within Pf1N1B4_818 at 936.032 kb on + strand, within Pf1N1B4_818 at 936.032 kb on + strand, within Pf1N1B4_818 at 936.032 kb on + strand, within Pf1N1B4_818 at 936.032 kb on + strand, within Pf1N1B4_818 at 936.032 kb on + strand, within Pf1N1B4_818 at 936.032 kb on + strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.033 kb on - strand, within Pf1N1B4_818 at 936.085 kb on - strand, within Pf1N1B4_818 at 936.087 kb on - strand at 936.087 kb on - strand, within Pf1N1B4_818 at 936.215 kb on + strand at 936.215 kb on + strand at 936.215 kb on + strand at 936.215 kb on + strand at 936.215 kb on + strand at 936.215 kb on + strand at 936.215 kb on + strand at 936.216 kb on - strand at 936.216 kb on - strand at 936.216 kb on - strand at 936.216 kb on - strand at 936.216 kb on - strand at 936.216 kb on - strand at 936.216 kb on - strand at 936.239 kb on - strand at 936.298 kb on + strand at 936.320 kb on + strand at 936.324 kb on - strand at 936.484 kb on - strand at 936.627 kb on + strand at 936.627 kb on + strand at 936.627 kb on + strand at 936.627 kb on + strand at 936.628 kb on - strand at 936.674 kb on + strand at 936.674 kb on + strand at 936.821 kb on - strand, within Pf1N1B4_819 at 936.899 kb on + strand, within Pf1N1B4_819 at 936.899 kb on + strand, within Pf1N1B4_819 at 936.899 kb on + strand, within Pf1N1B4_819 at 936.899 kb on + strand, within Pf1N1B4_819 at 936.900 kb on - strand, within Pf1N1B4_819 at 936.900 kb on - strand at 936.900 kb on - strand, within Pf1N1B4_819 at 936.915 kb on + strand, within Pf1N1B4_819 at 936.920 kb on - strand, within Pf1N1B4_819 at 937.245 kb on + strand, within Pf1N1B4_819 at 937.249 kb on + strand, within Pf1N1B4_819 at 937.290 kb on - strand, within Pf1N1B4_819 at 937.290 kb on - strand, within Pf1N1B4_819 at 937.290 kb on - strand, within Pf1N1B4_819 at 937.326 kb on - strand at 937.372 kb on + strand, within Pf1N1B4_819 at 937.372 kb on + strand, within Pf1N1B4_819 at 937.372 kb on + strand, within Pf1N1B4_819 at 937.372 kb on + strand, within Pf1N1B4_819 at 937.373 kb on - strand, within Pf1N1B4_819 at 937.373 kb on - strand, within Pf1N1B4_819 at 937.472 kb on + strand, within Pf1N1B4_819 at 937.472 kb on + strand, within Pf1N1B4_819 at 937.472 kb on + strand, within Pf1N1B4_819 at 937.472 kb on + strand, within Pf1N1B4_819 at 937.472 kb on + strand, within Pf1N1B4_819 at 937.502 kb on + strand, within Pf1N1B4_819 at 937.502 kb on + strand, within Pf1N1B4_819 at 937.502 kb on + strand, within Pf1N1B4_819 at 937.502 kb on + strand, within Pf1N1B4_819 at 937.502 kb on + strand, within Pf1N1B4_819 at 937.502 kb on + strand, within Pf1N1B4_819 at 937.502 kb on + strand, within Pf1N1B4_819 at 937.502 kb on + strand, within Pf1N1B4_819 at 937.502 kb on + strand, within Pf1N1B4_819 at 937.503 kb on - strand, within Pf1N1B4_819 at 937.503 kb on - strand, within Pf1N1B4_819 at 937.503 kb on - strand, within Pf1N1B4_819 at 937.503 kb on - strand, within Pf1N1B4_819 at 937.503 kb on - strand, within Pf1N1B4_819 at 937.503 kb on - strand, within Pf1N1B4_819 at 937.760 kb on + strand at 937.760 kb on + strand at 937.760 kb on + strand at 937.760 kb on + strand at 937.760 kb on + strand at 937.760 kb on + strand at 937.760 kb on + strand at 937.760 kb on + strand at 937.760 kb on + strand at 937.761 kb on - strand at 937.761 kb on - strand at 937.761 kb on - strand at 937.761 kb on - strand at 937.837 kb on + strand at 937.837 kb on + strand at 937.838 kb on - strand at 937.838 kb on - strand at 937.865 kb on + strand at 937.865 kb on + strand at 937.865 kb on + strand at 937.866 kb on - strand at 937.866 kb on - strand at 937.968 kb on - strand at 937.968 kb on - strand at 937.968 kb on - strand at 937.999 kb on + strand at 938.096 kb on + strand at 938.096 kb on + strand at 938.310 kb on + strand, within Pf1N1B4_820 at 938.311 kb on - strand, within Pf1N1B4_820 at 938.335 kb on + strand, within Pf1N1B4_820 at 938.335 kb on + strand, within Pf1N1B4_820 at 938.335 kb on + strand, within Pf1N1B4_820 at 938.335 kb on + strand at 938.336 kb on - strand at 938.336 kb on - strand, within Pf1N1B4_820 at 938.336 kb on - strand, within Pf1N1B4_820 at 938.574 kb on + strand, within Pf1N1B4_820 at 938.575 kb on - strand, within Pf1N1B4_820 at 938.575 kb on - strand, within Pf1N1B4_820 at 938.718 kb on + strand, within Pf1N1B4_820 at 938.718 kb on + strand, within Pf1N1B4_820 at 938.719 kb on - strand, within Pf1N1B4_820 at 938.757 kb on + strand, within Pf1N1B4_820 at 938.757 kb on + strand, within Pf1N1B4_820 at 938.757 kb on + strand, within Pf1N1B4_820 at 938.757 kb on + strand, within Pf1N1B4_820 at 938.758 kb on - strand, within Pf1N1B4_820 at 938.758 kb on - strand, within Pf1N1B4_820 at 938.758 kb on - strand, within Pf1N1B4_820 at 938.797 kb on - strand, within Pf1N1B4_820
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 935,850 - Pf1N1B4_818 0.71 -0.4 935,913 - Pf1N1B4_818 0.75 -2.4 935,931 - Pf1N1B4_818 0.77 -0.7 935,931 - Pf1N1B4_818 0.77 +0.3 935,931 - Pf1N1B4_818 0.77 +0.7 935,931 - Pf1N1B4_818 0.77 +1.5 935,931 - Pf1N1B4_818 0.77 -0.2 935,957 - Pf1N1B4_818 0.78 +0.3 935,992 - Pf1N1B4_818 0.81 -2.6 935,992 - Pf1N1B4_818 0.81 +0.3 936,028 + Pf1N1B4_818 0.84 +0.2 936,028 + Pf1N1B4_818 0.84 +0.3 936,028 + Pf1N1B4_818 0.84 -0.8 936,029 - Pf1N1B4_818 0.84 -0.1 936,029 - Pf1N1B4_818 0.84 +1.2 936,029 - Pf1N1B4_818 0.84 -1.5 936,029 - Pf1N1B4_818 0.84 -2.0 936,029 - Pf1N1B4_818 0.84 -2.3 936,032 + Pf1N1B4_818 0.84 -1.8 936,032 + Pf1N1B4_818 0.84 +0.4 936,032 + Pf1N1B4_818 0.84 +1.4 936,032 + Pf1N1B4_818 0.84 -2.2 936,032 + Pf1N1B4_818 0.84 -0.5 936,032 + Pf1N1B4_818 0.84 -1.1 936,032 + Pf1N1B4_818 0.84 -2.2 936,033 - Pf1N1B4_818 0.84 +0.2 936,033 - Pf1N1B4_818 0.84 +1.0 936,033 - Pf1N1B4_818 0.84 -0.4 936,033 - Pf1N1B4_818 0.84 +0.5 936,033 - Pf1N1B4_818 0.84 +0.8 936,033 - Pf1N1B4_818 0.84 -1.4 936,033 - Pf1N1B4_818 0.84 -0.7 936,033 - Pf1N1B4_818 0.84 -0.7 936,033 - Pf1N1B4_818 0.84 +1.0 936,033 - Pf1N1B4_818 0.84 +0.4 936,033 - Pf1N1B4_818 0.84 -1.1 936,033 - Pf1N1B4_818 0.84 -1.6 936,033 - Pf1N1B4_818 0.84 +1.6 936,085 - Pf1N1B4_818 0.88 +0.7 936,087 - +1.8 936,087 - Pf1N1B4_818 0.88 +0.3 936,215 + -0.7 936,215 + +1.1 936,215 + +0.1 936,215 + +0.2 936,215 + -0.4 936,215 + -1.2 936,215 + -0.8 936,216 - -1.2 936,216 - +0.1 936,216 - -0.1 936,216 - -1.1 936,216 - -1.7 936,216 - +0.3 936,216 - -2.9 936,239 - +0.6 936,298 + -1.2 936,320 + -1.9 936,324 - +2.3 936,484 - -0.6 936,627 + +0.5 936,627 + +0.0 936,627 + -2.8 936,627 + +0.8 936,628 - -3.7 936,674 + +0.1 936,674 + +0.4 936,821 - Pf1N1B4_819 0.18 +0.8 936,899 + Pf1N1B4_819 0.24 +3.0 936,899 + Pf1N1B4_819 0.24 -0.9 936,899 + Pf1N1B4_819 0.24 +0.3 936,899 + Pf1N1B4_819 0.24 -0.5 936,900 - Pf1N1B4_819 0.24 +0.5 936,900 - +1.3 936,900 - Pf1N1B4_819 0.24 -2.2 936,915 + Pf1N1B4_819 0.26 +1.7 936,920 - Pf1N1B4_819 0.26 -0.2 937,245 + Pf1N1B4_819 0.53 +0.1 937,249 + Pf1N1B4_819 0.53 +0.6 937,290 - Pf1N1B4_819 0.57 -0.1 937,290 - Pf1N1B4_819 0.57 -1.5 937,290 - Pf1N1B4_819 0.57 -0.1 937,326 - -1.1 937,372 + Pf1N1B4_819 0.64 -0.4 937,372 + Pf1N1B4_819 0.64 -0.8 937,372 + Pf1N1B4_819 0.64 -0.9 937,372 + Pf1N1B4_819 0.64 -0.5 937,373 - Pf1N1B4_819 0.64 -2.4 937,373 - Pf1N1B4_819 0.64 -1.0 937,472 + Pf1N1B4_819 0.72 -0.2 937,472 + Pf1N1B4_819 0.72 -0.7 937,472 + Pf1N1B4_819 0.72 -2.7 937,472 + Pf1N1B4_819 0.72 -0.3 937,472 + Pf1N1B4_819 0.72 +1.0 937,502 + Pf1N1B4_819 0.74 -0.6 937,502 + Pf1N1B4_819 0.74 -0.4 937,502 + Pf1N1B4_819 0.74 -0.2 937,502 + Pf1N1B4_819 0.74 -0.7 937,502 + Pf1N1B4_819 0.74 +0.8 937,502 + Pf1N1B4_819 0.74 +2.1 937,502 + Pf1N1B4_819 0.74 -1.0 937,502 + Pf1N1B4_819 0.74 +0.6 937,502 + Pf1N1B4_819 0.74 -1.8 937,503 - Pf1N1B4_819 0.74 +2.6 937,503 - Pf1N1B4_819 0.74 -1.4 937,503 - Pf1N1B4_819 0.74 +0.0 937,503 - Pf1N1B4_819 0.74 +1.4 937,503 - Pf1N1B4_819 0.74 -1.1 937,503 - Pf1N1B4_819 0.74 -1.2 937,760 + +0.9 937,760 + +2.3 937,760 + +0.6 937,760 + -1.0 937,760 + +0.9 937,760 + -1.2 937,760 + -1.4 937,760 + -0.3 937,760 + -0.5 937,761 - -1.2 937,761 - -3.8 937,761 - +0.3 937,761 - -1.4 937,837 + +1.5 937,837 + +0.5 937,838 - -0.7 937,838 - -0.8 937,865 + +1.6 937,865 + +0.8 937,865 + -0.0 937,866 - -0.7 937,866 - +0.4 937,968 - -2.4 937,968 - -3.6 937,968 - -0.9 937,999 + +0.8 938,096 + +2.5 938,096 + +0.4 938,310 + Pf1N1B4_820 0.13 -0.3 938,311 - Pf1N1B4_820 0.13 +0.0 938,335 + Pf1N1B4_820 0.14 -0.2 938,335 + Pf1N1B4_820 0.14 -1.6 938,335 + Pf1N1B4_820 0.14 -0.5 938,335 + -1.1 938,336 - -0.2 938,336 - Pf1N1B4_820 0.14 -2.1 938,336 - Pf1N1B4_820 0.14 -0.8 938,574 + Pf1N1B4_820 0.32 -0.1 938,575 - Pf1N1B4_820 0.32 -1.8 938,575 - Pf1N1B4_820 0.32 -2.8 938,718 + Pf1N1B4_820 0.43 +1.3 938,718 + Pf1N1B4_820 0.43 -1.6 938,719 - Pf1N1B4_820 0.43 -0.9 938,757 + Pf1N1B4_820 0.46 -0.1 938,757 + Pf1N1B4_820 0.46 -2.0 938,757 + Pf1N1B4_820 0.46 +1.9 938,757 + Pf1N1B4_820 0.46 -0.8 938,758 - Pf1N1B4_820 0.46 -1.2 938,758 - Pf1N1B4_820 0.46 -2.0 938,758 - Pf1N1B4_820 0.46 -2.5 938,797 - Pf1N1B4_820 0.49 -2.3
Or see this region's nucleotide sequence