Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_690

Experiment: nitrogen source nitrate 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_688 and Pf1N1B4_689 are separated by 41 nucleotidesPf1N1B4_689 and Pf1N1B4_690 are separated by 427 nucleotidesPf1N1B4_690 and Pf1N1B4_691 are separated by 129 nucleotides Pf1N1B4_688 - FIG00955483: hypothetical protein, at 787,083 to 787,313 _688 Pf1N1B4_689 - FIG00954888: hypothetical protein, at 787,355 to 787,708 _689 Pf1N1B4_690 - Transcriptional regulator, AraC family, at 788,136 to 789,065 _690 Pf1N1B4_691 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 789,195 to 790,250 _691 Position (kb) 788 789 790Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 787.269 kb on + strand, within Pf1N1B4_688at 787.269 kb on + strand, within Pf1N1B4_688at 787.269 kb on + strand, within Pf1N1B4_688at 787.269 kb on + strand, within Pf1N1B4_688at 787.269 kb on + strand, within Pf1N1B4_688at 787.373 kb on + strandat 787.374 kb on - strandat 787.500 kb on + strand, within Pf1N1B4_689at 787.500 kb on + strand, within Pf1N1B4_689at 787.500 kb on + strand, within Pf1N1B4_689at 787.501 kb on - strand, within Pf1N1B4_689at 787.681 kb on + strandat 787.682 kb on - strandat 787.682 kb on - strandat 787.702 kb on + strandat 787.703 kb on - strandat 787.870 kb on + strandat 787.870 kb on + strandat 788.095 kb on - strandat 788.220 kb on - strandat 788.241 kb on + strand, within Pf1N1B4_690at 788.241 kb on + strand, within Pf1N1B4_690at 788.242 kb on - strandat 788.242 kb on - strand, within Pf1N1B4_690at 788.242 kb on - strand, within Pf1N1B4_690at 788.242 kb on - strand, within Pf1N1B4_690at 788.242 kb on - strand, within Pf1N1B4_690at 788.370 kb on + strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.371 kb on - strand, within Pf1N1B4_690at 788.379 kb on + strand, within Pf1N1B4_690at 788.380 kb on - strand, within Pf1N1B4_690at 788.395 kb on - strand, within Pf1N1B4_690at 788.395 kb on - strand, within Pf1N1B4_690at 788.395 kb on - strand, within Pf1N1B4_690at 788.438 kb on - strand, within Pf1N1B4_690at 788.438 kb on - strand, within Pf1N1B4_690at 788.438 kb on - strand, within Pf1N1B4_690at 788.438 kb on - strand, within Pf1N1B4_690at 788.562 kb on + strand, within Pf1N1B4_690at 788.562 kb on + strand, within Pf1N1B4_690at 788.562 kb on + strand, within Pf1N1B4_690at 788.562 kb on + strand, within Pf1N1B4_690at 788.563 kb on - strand, within Pf1N1B4_690at 788.781 kb on + strand, within Pf1N1B4_690at 788.782 kb on - strand, within Pf1N1B4_690at 788.782 kb on - strand, within Pf1N1B4_690at 788.782 kb on - strand, within Pf1N1B4_690at 788.886 kb on + strand, within Pf1N1B4_690at 789.003 kb on + strandat 789.003 kb on + strandat 789.130 kb on + strandat 789.130 kb on + strandat 789.131 kb on - strandat 789.282 kb on - strandat 789.328 kb on - strand, within Pf1N1B4_691at 789.328 kb on - strand, within Pf1N1B4_691at 789.552 kb on - strand, within Pf1N1B4_691at 789.552 kb on - strand, within Pf1N1B4_691at 789.586 kb on + strand, within Pf1N1B4_691at 789.587 kb on - strand, within Pf1N1B4_691at 790.025 kb on + strand, within Pf1N1B4_691at 790.044 kb on - strand, within Pf1N1B4_691at 790.044 kb on - strand, within Pf1N1B4_691

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM
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787,269 + Pf1N1B4_688 0.81 -1.0
787,269 + Pf1N1B4_688 0.81 -0.3
787,269 + Pf1N1B4_688 0.81 -0.7
787,269 + Pf1N1B4_688 0.81 +0.5
787,269 + Pf1N1B4_688 0.81 -1.4
787,373 + -3.0
787,374 - -0.2
787,500 + Pf1N1B4_689 0.41 +0.3
787,500 + Pf1N1B4_689 0.41 +1.0
787,500 + Pf1N1B4_689 0.41 +0.3
787,501 - Pf1N1B4_689 0.41 -2.5
787,681 + -0.6
787,682 - +0.4
787,682 - -1.6
787,702 + +0.9
787,703 - +0.2
787,870 + -2.2
787,870 + -1.1
788,095 - -0.3
788,220 - +1.4
788,241 + Pf1N1B4_690 0.11 +0.5
788,241 + Pf1N1B4_690 0.11 +1.8
788,242 - -1.4
788,242 - Pf1N1B4_690 0.11 +0.5
788,242 - Pf1N1B4_690 0.11 -1.4
788,242 - Pf1N1B4_690 0.11 -2.8
788,242 - Pf1N1B4_690 0.11 +0.2
788,370 + Pf1N1B4_690 0.25 +1.0
788,371 - Pf1N1B4_690 0.25 +1.4
788,371 - Pf1N1B4_690 0.25 +0.0
788,371 - Pf1N1B4_690 0.25 -0.6
788,371 - Pf1N1B4_690 0.25 +2.1
788,371 - Pf1N1B4_690 0.25 +1.0
788,371 - Pf1N1B4_690 0.25 +1.1
788,379 + Pf1N1B4_690 0.26 -2.5
788,380 - Pf1N1B4_690 0.26 -1.4
788,395 - Pf1N1B4_690 0.28 +0.1
788,395 - Pf1N1B4_690 0.28 -0.7
788,395 - Pf1N1B4_690 0.28 +2.3
788,438 - Pf1N1B4_690 0.32 +3.2
788,438 - Pf1N1B4_690 0.32 +0.1
788,438 - Pf1N1B4_690 0.32 -0.4
788,438 - Pf1N1B4_690 0.32 +0.1
788,562 + Pf1N1B4_690 0.46 -1.6
788,562 + Pf1N1B4_690 0.46 -1.0
788,562 + Pf1N1B4_690 0.46 -1.7
788,562 + Pf1N1B4_690 0.46 +1.5
788,563 - Pf1N1B4_690 0.46 +0.9
788,781 + Pf1N1B4_690 0.69 -2.1
788,782 - Pf1N1B4_690 0.69 -0.9
788,782 - Pf1N1B4_690 0.69 +0.8
788,782 - Pf1N1B4_690 0.69 -0.6
788,886 + Pf1N1B4_690 0.81 +0.8
789,003 + -0.0
789,003 + -1.4
789,130 + -2.2
789,130 + +0.8
789,131 - +2.3
789,282 - -1.0
789,328 - Pf1N1B4_691 0.13 -1.6
789,328 - Pf1N1B4_691 0.13 -1.2
789,552 - Pf1N1B4_691 0.34 +0.8
789,552 - Pf1N1B4_691 0.34 +0.6
789,586 + Pf1N1B4_691 0.37 -0.8
789,587 - Pf1N1B4_691 0.37 +0.6
790,025 + Pf1N1B4_691 0.79 +0.3
790,044 - Pf1N1B4_691 0.80 -0.7
790,044 - Pf1N1B4_691 0.80 -1.8

Or see this region's nucleotide sequence