Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5970 and Pf1N1B4_5971 are separated by 10 nucleotides Pf1N1B4_5971 and Pf1N1B4_5972 overlap by 8 nucleotides Pf1N1B4_5972 and Pf1N1B4_5973 are separated by 10 nucleotides
Pf1N1B4_5970 - ABC transporter, substrate binding protein, at 417,897 to 418,715
_5970
Pf1N1B4_5971 - 2-Methylcitrate dehydratase AcnD, at 418,726 to 420,504
_5971
Pf1N1B4_5972 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 420,497 to 421,162
_5972
Pf1N1B4_5973 - Probable permease of ABC transporter, at 421,173 to 421,823
_5973
Position (kb) 418
419
420
421 Strain fitness (log2 ratio) -2
-1
0
1
2 at 417.772 kb on - strand at 417.790 kb on - strand at 417.897 kb on - strand at 418.084 kb on - strand, within Pf1N1B4_5970 at 418.246 kb on - strand, within Pf1N1B4_5970 at 418.296 kb on - strand, within Pf1N1B4_5970 at 418.344 kb on + strand, within Pf1N1B4_5970 at 418.345 kb on - strand, within Pf1N1B4_5970 at 418.345 kb on - strand, within Pf1N1B4_5970 at 418.506 kb on + strand at 418.507 kb on - strand, within Pf1N1B4_5970 at 418.507 kb on - strand, within Pf1N1B4_5970 at 418.507 kb on - strand, within Pf1N1B4_5970 at 418.507 kb on - strand at 418.596 kb on + strand, within Pf1N1B4_5970 at 418.638 kb on + strand at 418.707 kb on + strand at 418.707 kb on + strand at 418.708 kb on - strand at 418.708 kb on - strand at 418.710 kb on + strand at 418.710 kb on + strand at 418.711 kb on - strand at 418.711 kb on - strand at 418.711 kb on - strand at 418.713 kb on + strand at 418.713 kb on + strand at 418.713 kb on + strand at 418.714 kb on - strand at 418.714 kb on - strand at 418.834 kb on + strand at 418.879 kb on + strand at 419.359 kb on - strand, within Pf1N1B4_5971 at 419.359 kb on - strand, within Pf1N1B4_5971 at 419.359 kb on - strand, within Pf1N1B4_5971 at 419.359 kb on - strand, within Pf1N1B4_5971 at 419.362 kb on + strand at 419.362 kb on + strand, within Pf1N1B4_5971 at 419.363 kb on - strand, within Pf1N1B4_5971 at 419.363 kb on - strand, within Pf1N1B4_5971 at 419.363 kb on - strand, within Pf1N1B4_5971 at 419.363 kb on - strand, within Pf1N1B4_5971 at 419.527 kb on + strand at 419.527 kb on + strand, within Pf1N1B4_5971 at 419.527 kb on + strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand at 419.528 kb on - strand at 419.528 kb on - strand at 419.529 kb on + strand, within Pf1N1B4_5971 at 419.680 kb on + strand, within Pf1N1B4_5971 at 419.680 kb on + strand, within Pf1N1B4_5971 at 419.819 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.192 kb on + strand at 420.352 kb on + strand at 420.352 kb on + strand at 420.352 kb on + strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.507 kb on - strand at 420.507 kb on - strand at 420.720 kb on - strand, within Pf1N1B4_5972 at 420.720 kb on - strand, within Pf1N1B4_5972 at 420.729 kb on - strand, within Pf1N1B4_5972 at 421.040 kb on + strand, within Pf1N1B4_5972 at 421.041 kb on - strand, within Pf1N1B4_5972 at 421.094 kb on + strand, within Pf1N1B4_5972 at 421.095 kb on - strand, within Pf1N1B4_5972 at 421.095 kb on - strand, within Pf1N1B4_5972 at 421.098 kb on - strand at 421.458 kb on + strand, within Pf1N1B4_5973 at 421.470 kb on + strand, within Pf1N1B4_5973
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 417,772 - +1.2 417,790 - -1.0 417,897 - +0.2 418,084 - Pf1N1B4_5970 0.23 +0.5 418,246 - Pf1N1B4_5970 0.43 +0.3 418,296 - Pf1N1B4_5970 0.49 -0.2 418,344 + Pf1N1B4_5970 0.55 -0.5 418,345 - Pf1N1B4_5970 0.55 +1.6 418,345 - Pf1N1B4_5970 0.55 +0.4 418,506 + -0.4 418,507 - Pf1N1B4_5970 0.74 +0.8 418,507 - Pf1N1B4_5970 0.74 -0.0 418,507 - Pf1N1B4_5970 0.74 +0.2 418,507 - +0.4 418,596 + Pf1N1B4_5970 0.85 -0.5 418,638 + -2.2 418,707 + -0.0 418,707 + +0.3 418,708 - -0.7 418,708 - -0.1 418,710 + -0.5 418,710 + -1.0 418,711 - +0.9 418,711 - +0.1 418,711 - -0.8 418,713 + +0.2 418,713 + +0.5 418,713 + -0.5 418,714 - +0.3 418,714 - -0.6 418,834 + -0.9 418,879 + +0.6 419,359 - Pf1N1B4_5971 0.36 -1.3 419,359 - Pf1N1B4_5971 0.36 +1.1 419,359 - Pf1N1B4_5971 0.36 +0.9 419,359 - Pf1N1B4_5971 0.36 +0.7 419,362 + +0.6 419,362 + Pf1N1B4_5971 0.36 +0.5 419,363 - Pf1N1B4_5971 0.36 -0.6 419,363 - Pf1N1B4_5971 0.36 +1.3 419,363 - Pf1N1B4_5971 0.36 -1.6 419,363 - Pf1N1B4_5971 0.36 +0.2 419,527 + -0.4 419,527 + Pf1N1B4_5971 0.45 +0.5 419,527 + Pf1N1B4_5971 0.45 -0.6 419,528 - Pf1N1B4_5971 0.45 +0.2 419,528 - Pf1N1B4_5971 0.45 -0.3 419,528 - Pf1N1B4_5971 0.45 -1.3 419,528 - Pf1N1B4_5971 0.45 +1.9 419,528 - Pf1N1B4_5971 0.45 -0.3 419,528 - -1.6 419,528 - -1.1 419,528 - -2.1 419,529 + Pf1N1B4_5971 0.45 +0.5 419,680 + Pf1N1B4_5971 0.54 +0.9 419,680 + Pf1N1B4_5971 0.54 +0.3 419,819 - Pf1N1B4_5971 0.61 +2.4 420,042 - Pf1N1B4_5971 0.74 +0.2 420,042 - Pf1N1B4_5971 0.74 +0.2 420,042 - Pf1N1B4_5971 0.74 +0.3 420,042 - Pf1N1B4_5971 0.74 +0.5 420,192 + -1.8 420,352 + +0.7 420,352 + +0.2 420,352 + +0.6 420,353 - +0.3 420,353 - -2.0 420,353 - +1.5 420,353 - -0.3 420,507 - -0.4 420,507 - +0.7 420,720 - Pf1N1B4_5972 0.33 +0.4 420,720 - Pf1N1B4_5972 0.33 -0.9 420,729 - Pf1N1B4_5972 0.35 -0.0 421,040 + Pf1N1B4_5972 0.82 +0.5 421,041 - Pf1N1B4_5972 0.82 -1.5 421,094 + Pf1N1B4_5972 0.90 +0.3 421,095 - Pf1N1B4_5972 0.90 -1.0 421,095 - Pf1N1B4_5972 0.90 -1.1 421,098 - -0.5 421,458 + Pf1N1B4_5973 0.44 -0.8 421,470 + Pf1N1B4_5973 0.46 -0.9
Or see this region's nucleotide sequence