Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_596 and Pf1N1B4_597 are separated by 129 nucleotides Pf1N1B4_597 and Pf1N1B4_598 are separated by 228 nucleotides
Pf1N1B4_596 - Glucose ABC transport system, periplasmic sugar-binding protein, at 687,656 to 688,963
_596
Pf1N1B4_597 - D-mannose isomerase (EC 5.3.1.7) <small>(from data)</small>, at 689,093 to 690,352
_597
Pf1N1B4_598 - Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster, at 690,581 to 692,050
_598
Position (kb) 689
690
691 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 688.311 kb on + strand, within Pf1N1B4_596 at 688.311 kb on + strand, within Pf1N1B4_596 at 688.311 kb on + strand, within Pf1N1B4_596 at 688.431 kb on + strand, within Pf1N1B4_596 at 688.432 kb on - strand, within Pf1N1B4_596 at 688.432 kb on - strand, within Pf1N1B4_596 at 688.551 kb on + strand, within Pf1N1B4_596 at 688.551 kb on + strand, within Pf1N1B4_596 at 688.551 kb on + strand, within Pf1N1B4_596 at 688.551 kb on + strand, within Pf1N1B4_596 at 688.551 kb on + strand, within Pf1N1B4_596 at 688.965 kb on + strand at 688.965 kb on + strand at 688.965 kb on + strand at 688.965 kb on + strand at 688.965 kb on + strand at 688.965 kb on + strand at 688.966 kb on - strand at 688.966 kb on - strand at 688.968 kb on + strand at 688.968 kb on + strand at 688.969 kb on - strand at 688.986 kb on + strand at 688.986 kb on + strand at 688.987 kb on - strand at 688.987 kb on - strand at 689.016 kb on + strand at 689.017 kb on - strand at 689.017 kb on - strand at 689.025 kb on - strand at 689.025 kb on - strand at 689.034 kb on - strand at 689.083 kb on - strand at 689.083 kb on - strand at 689.083 kb on - strand at 689.086 kb on + strand at 689.086 kb on + strand at 689.086 kb on + strand at 689.086 kb on + strand at 689.086 kb on + strand at 689.086 kb on + strand at 689.086 kb on + strand at 689.086 kb on + strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.087 kb on - strand at 689.188 kb on - strand at 689.322 kb on + strand, within Pf1N1B4_597 at 689.322 kb on + strand, within Pf1N1B4_597 at 689.323 kb on - strand, within Pf1N1B4_597 at 689.434 kb on - strand, within Pf1N1B4_597 at 689.434 kb on - strand, within Pf1N1B4_597 at 689.434 kb on - strand, within Pf1N1B4_597 at 689.524 kb on + strand at 689.524 kb on + strand, within Pf1N1B4_597 at 689.524 kb on + strand, within Pf1N1B4_597 at 689.524 kb on + strand, within Pf1N1B4_597 at 689.524 kb on + strand, within Pf1N1B4_597 at 689.524 kb on + strand, within Pf1N1B4_597 at 689.524 kb on + strand, within Pf1N1B4_597 at 689.524 kb on + strand, within Pf1N1B4_597 at 689.524 kb on + strand, within Pf1N1B4_597 at 689.525 kb on - strand, within Pf1N1B4_597 at 689.525 kb on - strand, within Pf1N1B4_597 at 689.525 kb on - strand, within Pf1N1B4_597 at 689.525 kb on - strand, within Pf1N1B4_597 at 689.525 kb on - strand, within Pf1N1B4_597 at 689.525 kb on - strand, within Pf1N1B4_597 at 689.525 kb on - strand, within Pf1N1B4_597 at 689.525 kb on - strand, within Pf1N1B4_597 at 689.525 kb on - strand, within Pf1N1B4_597 at 689.528 kb on + strand, within Pf1N1B4_597 at 689.529 kb on - strand, within Pf1N1B4_597 at 689.637 kb on + strand, within Pf1N1B4_597 at 689.638 kb on - strand, within Pf1N1B4_597 at 689.643 kb on + strand, within Pf1N1B4_597 at 689.643 kb on + strand at 689.644 kb on - strand, within Pf1N1B4_597 at 689.644 kb on - strand, within Pf1N1B4_597 at 689.644 kb on - strand, within Pf1N1B4_597 at 689.691 kb on + strand, within Pf1N1B4_597 at 689.692 kb on - strand, within Pf1N1B4_597 at 689.692 kb on - strand, within Pf1N1B4_597 at 689.692 kb on - strand, within Pf1N1B4_597 at 689.692 kb on - strand, within Pf1N1B4_597 at 689.829 kb on + strand, within Pf1N1B4_597 at 689.857 kb on + strand, within Pf1N1B4_597 at 690.000 kb on + strand, within Pf1N1B4_597 at 690.000 kb on + strand, within Pf1N1B4_597 at 690.163 kb on + strand, within Pf1N1B4_597 at 690.164 kb on - strand, within Pf1N1B4_597 at 690.164 kb on - strand, within Pf1N1B4_597 at 690.373 kb on + strand at 690.373 kb on + strand at 690.374 kb on - strand at 690.374 kb on - strand at 690.374 kb on - strand at 690.390 kb on + strand at 690.391 kb on - strand at 690.432 kb on + strand at 690.432 kb on + strand at 690.432 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.469 kb on - strand at 690.469 kb on - strand at 690.469 kb on - strand at 690.469 kb on - strand at 690.593 kb on - strand at 690.847 kb on - strand, within Pf1N1B4_598 at 690.967 kb on - strand, within Pf1N1B4_598
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 688,311 + Pf1N1B4_596 0.50 -0.5 688,311 + Pf1N1B4_596 0.50 -0.5 688,311 + Pf1N1B4_596 0.50 -1.1 688,431 + Pf1N1B4_596 0.59 +0.3 688,432 - Pf1N1B4_596 0.59 -1.6 688,432 - Pf1N1B4_596 0.59 +0.5 688,551 + Pf1N1B4_596 0.68 -0.2 688,551 + Pf1N1B4_596 0.68 -1.2 688,551 + Pf1N1B4_596 0.68 -1.7 688,551 + Pf1N1B4_596 0.68 -0.9 688,551 + Pf1N1B4_596 0.68 -0.5 688,965 + -0.6 688,965 + -1.2 688,965 + -2.6 688,965 + -0.5 688,965 + -1.6 688,965 + +0.4 688,966 - -1.4 688,966 - -1.3 688,968 + -0.9 688,968 + -1.8 688,969 - +0.1 688,986 + -1.6 688,986 + -1.5 688,987 - +0.9 688,987 - +0.2 689,016 + -2.9 689,017 - +1.5 689,017 - -0.3 689,025 - -0.0 689,025 - +1.1 689,034 - -0.1 689,083 - -0.4 689,083 - +1.3 689,083 - +0.2 689,086 + -0.4 689,086 + +0.3 689,086 + +0.1 689,086 + +0.4 689,086 + -0.6 689,086 + +0.3 689,086 + -0.2 689,086 + +0.5 689,087 - -0.2 689,087 - -1.0 689,087 - -0.8 689,087 - -1.6 689,087 - -0.4 689,087 - +0.3 689,087 - +0.3 689,087 - +0.1 689,087 - +1.0 689,087 - -0.8 689,087 - +0.2 689,188 - +0.8 689,322 + Pf1N1B4_597 0.18 -0.5 689,322 + Pf1N1B4_597 0.18 +1.0 689,323 - Pf1N1B4_597 0.18 +1.0 689,434 - Pf1N1B4_597 0.27 -0.5 689,434 - Pf1N1B4_597 0.27 -1.7 689,434 - Pf1N1B4_597 0.27 +0.8 689,524 + +0.1 689,524 + Pf1N1B4_597 0.34 -0.1 689,524 + Pf1N1B4_597 0.34 -1.7 689,524 + Pf1N1B4_597 0.34 +1.1 689,524 + Pf1N1B4_597 0.34 -1.6 689,524 + Pf1N1B4_597 0.34 +0.2 689,524 + Pf1N1B4_597 0.34 -0.2 689,524 + Pf1N1B4_597 0.34 -0.2 689,524 + Pf1N1B4_597 0.34 +0.9 689,525 - Pf1N1B4_597 0.34 -0.8 689,525 - Pf1N1B4_597 0.34 +0.0 689,525 - Pf1N1B4_597 0.34 +1.4 689,525 - Pf1N1B4_597 0.34 -0.8 689,525 - Pf1N1B4_597 0.34 +2.5 689,525 - Pf1N1B4_597 0.34 +1.6 689,525 - Pf1N1B4_597 0.34 +0.0 689,525 - Pf1N1B4_597 0.34 +1.8 689,525 - Pf1N1B4_597 0.34 +0.9 689,528 + Pf1N1B4_597 0.35 +0.8 689,529 - Pf1N1B4_597 0.35 +0.2 689,637 + Pf1N1B4_597 0.43 -0.3 689,638 - Pf1N1B4_597 0.43 +1.2 689,643 + Pf1N1B4_597 0.44 +1.5 689,643 + -1.2 689,644 - Pf1N1B4_597 0.44 +0.8 689,644 - Pf1N1B4_597 0.44 -0.5 689,644 - Pf1N1B4_597 0.44 -0.1 689,691 + Pf1N1B4_597 0.47 -0.7 689,692 - Pf1N1B4_597 0.48 +0.4 689,692 - Pf1N1B4_597 0.48 +0.4 689,692 - Pf1N1B4_597 0.48 +0.4 689,692 - Pf1N1B4_597 0.48 -0.2 689,829 + Pf1N1B4_597 0.58 -2.1 689,857 + Pf1N1B4_597 0.61 +0.2 690,000 + Pf1N1B4_597 0.72 +0.1 690,000 + Pf1N1B4_597 0.72 +1.1 690,163 + Pf1N1B4_597 0.85 +0.5 690,164 - Pf1N1B4_597 0.85 +1.5 690,164 - Pf1N1B4_597 0.85 -0.5 690,373 + +0.2 690,373 + -0.5 690,374 - +1.2 690,374 - +0.8 690,374 - +0.5 690,390 + +0.0 690,391 - -0.1 690,432 + -0.8 690,432 + -0.1 690,432 + -2.3 690,468 + +0.0 690,468 + -0.7 690,468 + +0.6 690,468 + -0.3 690,468 + -1.2 690,468 + +0.4 690,468 + -0.9 690,468 + -1.4 690,468 + -2.8 690,468 + +0.1 690,469 - -0.3 690,469 - -0.1 690,469 - -0.5 690,469 - -0.1 690,593 - +0.5 690,847 - Pf1N1B4_598 0.18 -1.4 690,967 - Pf1N1B4_598 0.26 -1.7
Or see this region's nucleotide sequence