Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5907 and Pf1N1B4_5908 are separated by 89 nucleotides Pf1N1B4_5908 and Pf1N1B4_5909 are separated by 70 nucleotides Pf1N1B4_5909 and Pf1N1B4_5910 are separated by 9 nucleotides Pf1N1B4_5910 and Pf1N1B4_5911 overlap by 8 nucleotides
Pf1N1B4_5907 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 349,909 to 351,336
_5907
Pf1N1B4_5908 - Nicotinamidase family protein YcaC, at 351,426 to 352,052
_5908
Pf1N1B4_5909 - Exoenzymes regulatory protein AepA precursor, at 352,123 to 353,961
_5909
Pf1N1B4_5910 - putative membrane protein, at 353,971 to 354,561
_5910
Pf1N1B4_5911 - DoxX family protein, at 354,554 to 354,970
_5911
Position (kb) 352
353
354 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 351.166 kb on + strand, within Pf1N1B4_5907 at 351.167 kb on - strand, within Pf1N1B4_5907 at 351.367 kb on + strand at 351.367 kb on + strand at 351.367 kb on + strand at 351.391 kb on + strand at 351.391 kb on + strand at 351.469 kb on + strand at 351.709 kb on - strand, within Pf1N1B4_5908 at 352.005 kb on + strand at 352.037 kb on - strand at 352.037 kb on - strand at 352.079 kb on - strand at 352.079 kb on - strand at 352.124 kb on + strand at 352.124 kb on + strand at 352.124 kb on + strand at 352.617 kb on - strand, within Pf1N1B4_5909 at 352.617 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.638 kb on - strand, within Pf1N1B4_5909 at 352.752 kb on - strand, within Pf1N1B4_5909 at 352.752 kb on - strand at 352.931 kb on + strand, within Pf1N1B4_5909 at 352.931 kb on + strand at 353.183 kb on + strand, within Pf1N1B4_5909 at 353.184 kb on - strand, within Pf1N1B4_5909 at 353.237 kb on + strand, within Pf1N1B4_5909 at 353.525 kb on + strand, within Pf1N1B4_5909 at 353.732 kb on + strand, within Pf1N1B4_5909 at 354.048 kb on - strand, within Pf1N1B4_5910 at 354.098 kb on + strand, within Pf1N1B4_5910 at 354.801 kb on - strand at 354.858 kb on + strand, within Pf1N1B4_5911 at 354.858 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 351,166 + Pf1N1B4_5907 0.88 -0.2 351,167 - Pf1N1B4_5907 0.88 -0.8 351,367 + -2.3 351,367 + -0.2 351,367 + -0.1 351,391 + -0.1 351,391 + +0.0 351,469 + +0.2 351,709 - Pf1N1B4_5908 0.45 -2.2 352,005 + +0.7 352,037 - -0.8 352,037 - -0.7 352,079 - +0.7 352,079 - +0.3 352,124 + +3.1 352,124 + +1.3 352,124 + +1.6 352,617 - Pf1N1B4_5909 0.27 +0.9 352,617 - Pf1N1B4_5909 0.27 +0.6 352,638 - Pf1N1B4_5909 0.28 -0.0 352,638 - Pf1N1B4_5909 0.28 -2.7 352,638 - Pf1N1B4_5909 0.28 -0.0 352,638 - Pf1N1B4_5909 0.28 -0.9 352,638 - Pf1N1B4_5909 0.28 +2.2 352,752 - Pf1N1B4_5909 0.34 -1.1 352,752 - +1.4 352,931 + Pf1N1B4_5909 0.44 +1.1 352,931 + -2.1 353,183 + Pf1N1B4_5909 0.58 +1.7 353,184 - Pf1N1B4_5909 0.58 -0.3 353,237 + Pf1N1B4_5909 0.61 +1.1 353,525 + Pf1N1B4_5909 0.76 +0.5 353,732 + Pf1N1B4_5909 0.87 +0.3 354,048 - Pf1N1B4_5910 0.13 -0.5 354,098 + Pf1N1B4_5910 0.21 +0.9 354,801 - -0.4 354,858 + Pf1N1B4_5911 0.73 +0.1 354,858 + -0.3
Or see this region's nucleotide sequence