Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5759 and Pf1N1B4_5760 are separated by 3 nucleotides Pf1N1B4_5760 and Pf1N1B4_5761 are separated by 79 nucleotides Pf1N1B4_5761 and Pf1N1B4_5762 are separated by 11 nucleotides Pf1N1B4_5762 and Pf1N1B4_5763 are separated by 43 nucleotides
Pf1N1B4_5759 - Nitric-oxide reductase subunit B (EC 1.7.99.7), at 199,183 to 200,610
_5759
Pf1N1B4_5760 - Nitric-oxide reductase subunit C (EC 1.7.99.7), at 200,614 to 201,222
_5760
Pf1N1B4_5761 - Nitric oxide reductase activation protein NorF, at 201,302 to 201,559
_5761
Pf1N1B4_5762 - Nitric oxide reductase activation protein NorE, at 201,571 to 202,098
_5762
Pf1N1B4_5763 - Nitric oxide reductase activation protein NorQ, at 202,142 to 202,933
_5763
Position (kb) 201
202
203 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 200.615 kb on + strand at 200.616 kb on - strand at 200.640 kb on + strand at 200.641 kb on - strand at 200.641 kb on - strand at 200.770 kb on + strand, within Pf1N1B4_5760 at 200.770 kb on + strand, within Pf1N1B4_5760 at 200.770 kb on + strand, within Pf1N1B4_5760 at 201.015 kb on - strand at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.018 kb on + strand, within Pf1N1B4_5760 at 201.087 kb on + strand, within Pf1N1B4_5760 at 201.330 kb on - strand, within Pf1N1B4_5761 at 201.633 kb on - strand, within Pf1N1B4_5762 at 201.730 kb on + strand, within Pf1N1B4_5762 at 201.730 kb on + strand, within Pf1N1B4_5762 at 201.731 kb on - strand, within Pf1N1B4_5762 at 201.846 kb on - strand, within Pf1N1B4_5762 at 201.878 kb on + strand, within Pf1N1B4_5762 at 201.878 kb on + strand, within Pf1N1B4_5762 at 201.879 kb on - strand, within Pf1N1B4_5762 at 202.270 kb on + strand, within Pf1N1B4_5763 at 202.501 kb on - strand, within Pf1N1B4_5763 at 202.764 kb on + strand, within Pf1N1B4_5763 at 202.902 kb on + strand at 202.902 kb on + strand at 202.903 kb on - strand at 202.903 kb on - strand at 203.053 kb on - strand at 203.053 kb on - strand at 203.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 200,615 + -2.0 200,616 - +0.2 200,640 + -0.5 200,641 - -1.0 200,641 - -0.0 200,770 + Pf1N1B4_5760 0.26 -0.5 200,770 + Pf1N1B4_5760 0.26 +0.3 200,770 + Pf1N1B4_5760 0.26 +0.5 201,015 - -0.9 201,015 - Pf1N1B4_5760 0.66 -0.3 201,015 - Pf1N1B4_5760 0.66 -1.9 201,015 - Pf1N1B4_5760 0.66 -1.3 201,018 + Pf1N1B4_5760 0.66 +1.7 201,087 + Pf1N1B4_5760 0.78 -0.5 201,330 - Pf1N1B4_5761 0.11 +0.3 201,633 - Pf1N1B4_5762 0.12 +0.9 201,730 + Pf1N1B4_5762 0.30 -1.0 201,730 + Pf1N1B4_5762 0.30 +0.8 201,731 - Pf1N1B4_5762 0.30 +1.6 201,846 - Pf1N1B4_5762 0.52 +0.4 201,878 + Pf1N1B4_5762 0.58 -0.8 201,878 + Pf1N1B4_5762 0.58 +1.0 201,879 - Pf1N1B4_5762 0.58 +0.5 202,270 + Pf1N1B4_5763 0.16 -3.1 202,501 - Pf1N1B4_5763 0.45 -0.3 202,764 + Pf1N1B4_5763 0.79 +0.4 202,902 + -0.2 202,902 + +0.4 202,903 - +0.2 202,903 - +0.6 203,053 - +0.1 203,053 - -0.6 203,069 - +0.0
Or see this region's nucleotide sequence