Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5723 and Pf1N1B4_5724 are separated by 34 nucleotides Pf1N1B4_5724 and Pf1N1B4_5725 overlap by 16 nucleotides Pf1N1B4_5725 and Pf1N1B4_5726 are separated by 38 nucleotides Pf1N1B4_5726 and Pf1N1B4_5727 are separated by 15 nucleotides Pf1N1B4_5727 and Pf1N1B4_5728 are separated by 5 nucleotides Pf1N1B4_5728 and Pf1N1B4_5729 are separated by 32 nucleotides
Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424
_5723
Pf1N1B4_5724 - High-affinity iron permease, at 164,459 to 164,941
_5724
Pf1N1B4_5725 - High-affinity iron permease, at 164,926 to 165,300
_5725
Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667
_5726
Pf1N1B4_5727 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 166,683 to 167,369
_5727
Pf1N1B4_5728 - hypothetical protein, at 167,375 to 167,542
_5728
Pf1N1B4_5729 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 167,575 to 169,590
_5729
Position (kb) 165
166
167 Strain fitness (log2 ratio) -1
0
1
2 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.801 kb on + strand, within Pf1N1B4_5724 at 164.802 kb on - strand, within Pf1N1B4_5724 at 165.101 kb on - strand, within Pf1N1B4_5725 at 165.385 kb on + strand at 165.394 kb on - strand at 165.485 kb on - strand, within Pf1N1B4_5726 at 165.594 kb on + strand, within Pf1N1B4_5726 at 165.699 kb on + strand, within Pf1N1B4_5726 at 166.054 kb on + strand, within Pf1N1B4_5726 at 166.374 kb on + strand at 166.511 kb on - strand, within Pf1N1B4_5726 at 166.511 kb on - strand, within Pf1N1B4_5726 at 166.906 kb on + strand, within Pf1N1B4_5727
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 164,798 + Pf1N1B4_5724 0.70 -0.8 164,798 + Pf1N1B4_5724 0.70 +0.3 164,798 + Pf1N1B4_5724 0.70 +0.8 164,801 + Pf1N1B4_5724 0.71 +0.3 164,802 - Pf1N1B4_5724 0.71 -0.7 165,101 - Pf1N1B4_5725 0.47 +0.1 165,385 + -0.0 165,394 - -0.3 165,485 - Pf1N1B4_5726 0.11 +1.2 165,594 + Pf1N1B4_5726 0.19 +1.8 165,699 + Pf1N1B4_5726 0.27 +0.8 166,054 + Pf1N1B4_5726 0.54 -1.3 166,374 + +1.6 166,511 - Pf1N1B4_5726 0.88 +0.6 166,511 - Pf1N1B4_5726 0.88 -0.1 166,906 + Pf1N1B4_5727 0.32 -0.2
Or see this region's nucleotide sequence