Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5622 and Pf1N1B4_5623 are separated by 58 nucleotides Pf1N1B4_5623 and Pf1N1B4_5624 are separated by 339 nucleotides
Pf1N1B4_5622 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 48,458 to 49,546
_5622
Pf1N1B4_5623 - hypothetical protein, at 49,605 to 50,657
_5623
Pf1N1B4_5624 - Lipase precursor (EC 3.1.1.3), at 50,997 to 51,878
_5624
Position (kb)
49
50
51 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 48.761 kb on + strand, within Pf1N1B4_5622 at 48.807 kb on - strand, within Pf1N1B4_5622 at 49.205 kb on + strand at 49.206 kb on - strand, within Pf1N1B4_5622 at 49.621 kb on - strand at 49.621 kb on - strand at 49.727 kb on + strand, within Pf1N1B4_5623 at 49.728 kb on - strand, within Pf1N1B4_5623 at 49.732 kb on - strand, within Pf1N1B4_5623 at 49.732 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.043 kb on - strand, within Pf1N1B4_5623 at 50.298 kb on + strand, within Pf1N1B4_5623 at 50.339 kb on - strand at 50.348 kb on - strand, within Pf1N1B4_5623 at 50.348 kb on - strand at 50.389 kb on - strand, within Pf1N1B4_5623 at 50.389 kb on - strand, within Pf1N1B4_5623 at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.647 kb on + strand at 50.651 kb on + strand at 50.651 kb on + strand at 50.680 kb on + strand at 50.792 kb on - strand at 50.823 kb on + strand at 50.880 kb on - strand at 50.947 kb on - strand at 51.153 kb on - strand, within Pf1N1B4_5624 at 51.585 kb on + strand, within Pf1N1B4_5624 at 51.586 kb on - strand, within Pf1N1B4_5624
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 48,761 + Pf1N1B4_5622 0.28 -0.2 48,807 - Pf1N1B4_5622 0.32 +0.7 49,205 + -1.8 49,206 - Pf1N1B4_5622 0.69 +0.4 49,621 - +0.4 49,621 - -1.0 49,727 + Pf1N1B4_5623 0.12 +0.3 49,728 - Pf1N1B4_5623 0.12 -1.8 49,732 - Pf1N1B4_5623 0.12 -0.4 49,732 - Pf1N1B4_5623 0.12 +0.3 50,006 - Pf1N1B4_5623 0.38 +0.8 50,006 - Pf1N1B4_5623 0.38 -1.0 50,006 - Pf1N1B4_5623 0.38 -0.8 50,043 - Pf1N1B4_5623 0.42 -1.6 50,298 + Pf1N1B4_5623 0.66 +1.4 50,339 - +0.3 50,348 - Pf1N1B4_5623 0.71 +0.7 50,348 - +1.7 50,389 - Pf1N1B4_5623 0.74 +3.5 50,389 - Pf1N1B4_5623 0.74 +0.8 50,615 - +0.8 50,615 - -0.5 50,615 - +0.1 50,615 - -0.0 50,615 - +0.1 50,615 - +1.0 50,615 - +1.8 50,615 - +0.8 50,647 + -0.6 50,651 + -0.0 50,651 + -0.3 50,680 + -0.6 50,792 - +0.2 50,823 + +0.0 50,880 - +0.5 50,947 - +0.5 51,153 - Pf1N1B4_5624 0.18 -0.4 51,585 + Pf1N1B4_5624 0.67 +0.0 51,586 - Pf1N1B4_5624 0.67 +0.1
Or see this region's nucleotide sequence