Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5618 and Pf1N1B4_5619 are separated by 2 nucleotides Pf1N1B4_5619 and Pf1N1B4_5620 are separated by 184 nucleotides Pf1N1B4_5620 and Pf1N1B4_5621 are separated by 0 nucleotides
Pf1N1B4_5618 - Excinuclease ABC subunit C, at 44,031 to 45,854
_5618
Pf1N1B4_5619 - BarA-associated response regulator UvrY (= GacA = SirA), at 45,857 to 46,441
_5619
Pf1N1B4_5620 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 46,626 to 47,069
_5620
Pf1N1B4_5621 - Hydrolase, carbon-nitrogen family, at 47,070 to 47,828
_5621
Position (kb) 46
47
48 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 45.718 kb on + strand at 45.761 kb on - strand at 45.761 kb on - strand at 45.955 kb on + strand, within Pf1N1B4_5619 at 45.955 kb on + strand, within Pf1N1B4_5619 at 46.128 kb on + strand at 46.128 kb on + strand, within Pf1N1B4_5619 at 46.129 kb on - strand, within Pf1N1B4_5619 at 46.485 kb on - strand at 46.493 kb on - strand at 46.522 kb on + strand at 46.580 kb on + strand at 46.647 kb on - strand at 46.837 kb on - strand, within Pf1N1B4_5620 at 46.856 kb on - strand, within Pf1N1B4_5620 at 46.889 kb on - strand, within Pf1N1B4_5620 at 47.135 kb on + strand at 47.136 kb on - strand at 47.273 kb on - strand, within Pf1N1B4_5621 at 47.766 kb on - strand at 47.891 kb on + strand at 47.958 kb on + strand at 47.958 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 45,718 + -2.2 45,761 - -2.2 45,761 - -0.9 45,955 + Pf1N1B4_5619 0.17 -0.5 45,955 + Pf1N1B4_5619 0.17 -0.2 46,128 + -0.1 46,128 + Pf1N1B4_5619 0.46 -1.8 46,129 - Pf1N1B4_5619 0.46 -0.3 46,485 - -1.6 46,493 - -0.8 46,522 + -2.8 46,580 + -1.0 46,647 - +0.5 46,837 - Pf1N1B4_5620 0.48 -2.5 46,856 - Pf1N1B4_5620 0.52 -0.9 46,889 - Pf1N1B4_5620 0.59 -0.8 47,135 + +0.3 47,136 - -0.6 47,273 - Pf1N1B4_5621 0.27 -0.5 47,766 - +0.4 47,891 + +0.2 47,958 + +0.5 47,958 + +0.3
Or see this region's nucleotide sequence