Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5590 and Pf1N1B4_5591 are separated by 143 nucleotides Pf1N1B4_5591 and Pf1N1B4_5592 are separated by 15 nucleotides Pf1N1B4_5592 and Pf1N1B4_5593 overlap by 32 nucleotides
Pf1N1B4_5590 - Trans-feruloyl-CoA synthase (EC 6.2.1.34), at 11,783 to 13,666
_5590
Pf1N1B4_5591 - Vanillin dehydrogenase (Hydroxybenzaldehyde dehydrogenase) (EC 1.2.1.28), at 13,810 to 15,258
_5591
Pf1N1B4_5592 - hypothetical protein, at 15,274 to 15,417
_5592
Pf1N1B4_5593 - 4-hydroxycinnamoyl CoA hydratase/lyase (Enoyl-CoA hydratase/lyase) (EC 4.2.1.17), at 15,386 to 16,216
_5593
Position (kb) 13
14
15
16 Strain fitness (log2 ratio) -1
0
1
2 at 13.288 kb on - strand, within Pf1N1B4_5590 at 13.419 kb on + strand, within Pf1N1B4_5590 at 13.559 kb on + strand at 13.560 kb on - strand at 13.560 kb on - strand at 13.604 kb on + strand at 13.737 kb on + strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.965 kb on + strand, within Pf1N1B4_5591 at 14.184 kb on + strand, within Pf1N1B4_5591 at 14.878 kb on + strand, within Pf1N1B4_5591 at 14.878 kb on + strand at 14.878 kb on + strand, within Pf1N1B4_5591 at 14.879 kb on - strand, within Pf1N1B4_5591 at 15.273 kb on - strand at 15.291 kb on - strand, within Pf1N1B4_5592 at 15.508 kb on - strand, within Pf1N1B4_5593 at 15.708 kb on + strand, within Pf1N1B4_5593 at 15.708 kb on + strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.903 kb on + strand, within Pf1N1B4_5593 at 15.904 kb on - strand, within Pf1N1B4_5593 at 15.993 kb on + strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 16.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 13,288 - Pf1N1B4_5590 0.80 +0.0 13,419 + Pf1N1B4_5590 0.87 +0.6 13,559 + +0.4 13,560 - +1.9 13,560 - +0.0 13,604 + -0.6 13,737 + -0.3 13,744 - +1.2 13,744 - +0.1 13,744 - -0.9 13,744 - -0.3 13,744 - +0.7 13,965 + Pf1N1B4_5591 0.11 +1.3 14,184 + Pf1N1B4_5591 0.26 +1.4 14,878 + Pf1N1B4_5591 0.74 +1.5 14,878 + -0.3 14,878 + Pf1N1B4_5591 0.74 +1.7 14,879 - Pf1N1B4_5591 0.74 +0.7 15,273 - +0.3 15,291 - Pf1N1B4_5592 0.12 -0.6 15,508 - Pf1N1B4_5593 0.15 +0.8 15,708 + Pf1N1B4_5593 0.39 +0.5 15,708 + Pf1N1B4_5593 0.39 +0.3 15,709 - Pf1N1B4_5593 0.39 +0.2 15,709 - Pf1N1B4_5593 0.39 +0.0 15,709 - Pf1N1B4_5593 0.39 -0.4 15,709 - Pf1N1B4_5593 0.39 -0.5 15,709 - Pf1N1B4_5593 0.39 +1.1 15,903 + Pf1N1B4_5593 0.62 +0.3 15,904 - Pf1N1B4_5593 0.62 +0.2 15,993 + Pf1N1B4_5593 0.73 -0.0 15,994 - Pf1N1B4_5593 0.73 +1.0 15,994 - Pf1N1B4_5593 0.73 +0.0 15,994 - Pf1N1B4_5593 0.73 +0.3 16,206 + -0.2
Or see this region's nucleotide sequence