Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5561

Experiment: nitrogen source Ammonium chloride 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5559 and Pf1N1B4_5560 are separated by 324 nucleotidesPf1N1B4_5560 and Pf1N1B4_5561 are separated by 281 nucleotidesPf1N1B4_5561 and Pf1N1B4_5562 are separated by 16 nucleotides Pf1N1B4_5559 - hypothetical protein, at 329,864 to 329,986 _5559 Pf1N1B4_5560 - hypothetical protein, at 330,311 to 330,520 _5560 Pf1N1B4_5561 - hypothetical protein, at 330,802 to 331,050 _5561 Pf1N1B4_5562 - DNA/RNA non-specific endonuclease, at 331,067 to 331,942 _5562 Position (kb) 330 331 332Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 329.865 kb on + strandat 329.865 kb on + strandat 329.866 kb on - strandat 329.866 kb on - strandat 329.866 kb on - strandat 329.866 kb on - strandat 329.866 kb on - strandat 329.866 kb on - strandat 329.866 kb on - strandat 329.866 kb on - strandat 330.312 kb on + strandat 330.357 kb on - strand, within Pf1N1B4_5560at 330.357 kb on - strandat 330.474 kb on + strand, within Pf1N1B4_5560at 330.474 kb on + strand, within Pf1N1B4_5560at 330.535 kb on - strandat 330.563 kb on + strandat 330.563 kb on + strandat 330.803 kb on + strandat 330.803 kb on + strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.804 kb on - strandat 330.834 kb on + strand, within Pf1N1B4_5561at 330.835 kb on - strand, within Pf1N1B4_5561at 330.835 kb on - strand, within Pf1N1B4_5561at 330.846 kb on - strand, within Pf1N1B4_5561at 330.846 kb on - strand, within Pf1N1B4_5561at 330.846 kb on - strand, within Pf1N1B4_5561at 330.846 kb on - strand, within Pf1N1B4_5561at 330.900 kb on - strand, within Pf1N1B4_5561at 330.996 kb on - strand, within Pf1N1B4_5561at 330.996 kb on - strandat 330.996 kb on - strand, within Pf1N1B4_5561at 331.040 kb on + strandat 331.041 kb on - strandat 331.041 kb on - strandat 331.230 kb on + strand, within Pf1N1B4_5562at 331.230 kb on + strand, within Pf1N1B4_5562at 331.231 kb on - strand, within Pf1N1B4_5562at 331.231 kb on - strand, within Pf1N1B4_5562at 331.231 kb on - strand, within Pf1N1B4_5562at 331.302 kb on + strand, within Pf1N1B4_5562at 331.302 kb on + strand, within Pf1N1B4_5562at 331.435 kb on + strand, within Pf1N1B4_5562at 331.436 kb on - strand, within Pf1N1B4_5562at 331.436 kb on - strand, within Pf1N1B4_5562at 331.548 kb on + strand, within Pf1N1B4_5562at 331.549 kb on - strandat 331.642 kb on + strand, within Pf1N1B4_5562at 331.642 kb on + strandat 331.642 kb on + strand, within Pf1N1B4_5562at 331.642 kb on + strand, within Pf1N1B4_5562at 331.642 kb on + strand, within Pf1N1B4_5562at 331.643 kb on - strand, within Pf1N1B4_5562at 331.644 kb on + strand, within Pf1N1B4_5562at 331.644 kb on + strand, within Pf1N1B4_5562at 331.645 kb on - strand, within Pf1N1B4_5562at 331.645 kb on - strand, within Pf1N1B4_5562at 331.645 kb on - strand, within Pf1N1B4_5562at 331.645 kb on - strand, within Pf1N1B4_5562at 331.645 kb on - strand, within Pf1N1B4_5562at 331.687 kb on + strand, within Pf1N1B4_5562at 331.687 kb on + strand, within Pf1N1B4_5562at 331.687 kb on + strand, within Pf1N1B4_5562at 331.687 kb on + strand, within Pf1N1B4_5562at 331.687 kb on + strand, within Pf1N1B4_5562at 331.687 kb on + strandat 331.687 kb on + strand, within Pf1N1B4_5562at 331.687 kb on + strand, within Pf1N1B4_5562at 331.687 kb on + strand, within Pf1N1B4_5562at 331.688 kb on - strand, within Pf1N1B4_5562at 331.688 kb on - strand, within Pf1N1B4_5562at 331.688 kb on - strand, within Pf1N1B4_5562at 331.688 kb on - strand, within Pf1N1B4_5562at 331.688 kb on - strand, within Pf1N1B4_5562at 331.688 kb on - strand, within Pf1N1B4_5562at 331.688 kb on - strand, within Pf1N1B4_5562at 331.965 kb on + strandat 331.965 kb on + strandat 331.965 kb on + strandat 331.966 kb on - strandat 331.966 kb on - strandat 331.966 kb on - strandat 331.971 kb on + strandat 331.972 kb on - strandat 331.972 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM
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329,865 + +0.2
329,865 + +1.9
329,866 - -0.8
329,866 - +0.6
329,866 - -0.3
329,866 - -0.1
329,866 - +0.2
329,866 - -0.7
329,866 - -0.8
329,866 - -0.1
330,312 + +2.2
330,357 - Pf1N1B4_5560 0.22 -0.3
330,357 - -0.1
330,474 + Pf1N1B4_5560 0.78 -1.0
330,474 + Pf1N1B4_5560 0.78 -1.6
330,535 - +1.0
330,563 + +1.2
330,563 + +0.5
330,803 + +0.3
330,803 + -0.1
330,804 - +2.2
330,804 - -0.8
330,804 - -1.0
330,804 - -0.6
330,804 - +0.5
330,804 - -0.4
330,804 - +0.5
330,804 - -0.2
330,804 - +0.1
330,804 - -0.4
330,804 - -0.9
330,804 - -1.2
330,804 - -0.0
330,804 - +0.5
330,834 + Pf1N1B4_5561 0.13 -1.4
330,835 - Pf1N1B4_5561 0.13 -0.6
330,835 - Pf1N1B4_5561 0.13 -0.8
330,846 - Pf1N1B4_5561 0.18 +0.1
330,846 - Pf1N1B4_5561 0.18 -0.4
330,846 - Pf1N1B4_5561 0.18 +0.6
330,846 - Pf1N1B4_5561 0.18 -1.1
330,900 - Pf1N1B4_5561 0.39 -0.9
330,996 - Pf1N1B4_5561 0.78 +0.6
330,996 - +2.2
330,996 - Pf1N1B4_5561 0.78 -1.1
331,040 + -1.1
331,041 - -1.5
331,041 - +1.5
331,230 + Pf1N1B4_5562 0.19 -2.3
331,230 + Pf1N1B4_5562 0.19 +0.3
331,231 - Pf1N1B4_5562 0.19 -2.6
331,231 - Pf1N1B4_5562 0.19 -2.5
331,231 - Pf1N1B4_5562 0.19 +0.6
331,302 + Pf1N1B4_5562 0.27 -1.1
331,302 + Pf1N1B4_5562 0.27 -0.4
331,435 + Pf1N1B4_5562 0.42 +1.9
331,436 - Pf1N1B4_5562 0.42 +0.6
331,436 - Pf1N1B4_5562 0.42 +0.2
331,548 + Pf1N1B4_5562 0.55 +1.0
331,549 - +0.1
331,642 + Pf1N1B4_5562 0.66 -0.4
331,642 + +0.5
331,642 + Pf1N1B4_5562 0.66 +1.4
331,642 + Pf1N1B4_5562 0.66 -0.8
331,642 + Pf1N1B4_5562 0.66 -1.1
331,643 - Pf1N1B4_5562 0.66 +0.9
331,644 + Pf1N1B4_5562 0.66 +0.8
331,644 + Pf1N1B4_5562 0.66 -0.4
331,645 - Pf1N1B4_5562 0.66 -0.1
331,645 - Pf1N1B4_5562 0.66 +0.1
331,645 - Pf1N1B4_5562 0.66 +0.9
331,645 - Pf1N1B4_5562 0.66 -0.8
331,645 - Pf1N1B4_5562 0.66 -0.1
331,687 + Pf1N1B4_5562 0.71 +1.2
331,687 + Pf1N1B4_5562 0.71 +0.6
331,687 + Pf1N1B4_5562 0.71 -1.4
331,687 + Pf1N1B4_5562 0.71 -0.4
331,687 + Pf1N1B4_5562 0.71 -0.8
331,687 + -0.1
331,687 + Pf1N1B4_5562 0.71 -2.9
331,687 + Pf1N1B4_5562 0.71 +0.5
331,687 + Pf1N1B4_5562 0.71 +0.3
331,688 - Pf1N1B4_5562 0.71 -0.5
331,688 - Pf1N1B4_5562 0.71 +0.1
331,688 - Pf1N1B4_5562 0.71 -2.5
331,688 - Pf1N1B4_5562 0.71 -1.0
331,688 - Pf1N1B4_5562 0.71 +0.9
331,688 - Pf1N1B4_5562 0.71 -0.4
331,688 - Pf1N1B4_5562 0.71 +0.1
331,965 + -0.1
331,965 + -0.2
331,965 + -1.2
331,966 - -0.3
331,966 - -0.2
331,966 - -1.0
331,971 + -0.3
331,972 - +0.2
331,972 - -1.9

Or see this region's nucleotide sequence