Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5489 and Pf1N1B4_5490 are separated by 369 nucleotides Pf1N1B4_5490 and Pf1N1B4_5491 are separated by 335 nucleotides Pf1N1B4_5491 and Pf1N1B4_5492 are separated by 34 nucleotides Pf1N1B4_5492 and Pf1N1B4_5493 are separated by 91 nucleotides
Pf1N1B4_5489 - hypothetical protein, at 237,157 to 237,435
_5489
Pf1N1B4_5490 - hypothetical protein, at 237,805 to 238,038
_5490
Pf1N1B4_5491 - hypothetical protein, at 238,374 to 238,607
_5491
Pf1N1B4_5492 - hypothetical protein, at 238,642 to 238,875
_5492
Pf1N1B4_5493 - hypothetical protein, at 238,967 to 239,542
_5493
Position (kb) 237
238
239 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 236.914 kb on + strand at 236.914 kb on + strand at 236.914 kb on + strand at 236.915 kb on - strand at 236.915 kb on - strand at 236.925 kb on + strand at 236.925 kb on + strand at 236.926 kb on - strand at 236.926 kb on - strand at 236.926 kb on - strand at 236.965 kb on + strand at 236.970 kb on + strand at 237.092 kb on + strand at 237.092 kb on + strand at 237.111 kb on - strand at 237.111 kb on - strand at 237.135 kb on + strand at 237.136 kb on - strand at 237.136 kb on - strand at 237.139 kb on + strand at 237.140 kb on - strand at 237.140 kb on - strand at 237.140 kb on - strand at 237.175 kb on - strand at 237.256 kb on + strand, within Pf1N1B4_5489 at 237.256 kb on + strand, within Pf1N1B4_5489 at 237.257 kb on - strand, within Pf1N1B4_5489 at 237.257 kb on - strand, within Pf1N1B4_5489 at 237.257 kb on - strand, within Pf1N1B4_5489 at 237.257 kb on - strand, within Pf1N1B4_5489 at 237.257 kb on - strand, within Pf1N1B4_5489 at 237.257 kb on - strand, within Pf1N1B4_5489 at 237.257 kb on - strand, within Pf1N1B4_5489 at 237.480 kb on - strand at 237.480 kb on - strand at 237.480 kb on - strand at 237.480 kb on - strand at 237.522 kb on - strand at 237.531 kb on + strand at 237.538 kb on + strand at 237.538 kb on + strand at 237.538 kb on + strand at 237.538 kb on + strand at 237.538 kb on + strand at 237.539 kb on - strand at 237.539 kb on - strand at 237.539 kb on - strand at 237.539 kb on - strand at 237.539 kb on - strand at 237.539 kb on - strand at 237.539 kb on - strand at 237.539 kb on - strand at 237.539 kb on - strand at 237.548 kb on - strand at 237.548 kb on - strand at 237.548 kb on - strand at 237.647 kb on - strand at 237.663 kb on + strand at 237.663 kb on + strand at 237.663 kb on + strand at 237.663 kb on + strand at 237.664 kb on - strand at 237.664 kb on - strand at 237.664 kb on - strand at 237.664 kb on - strand at 237.710 kb on + strand at 237.710 kb on + strand at 237.710 kb on + strand at 237.711 kb on - strand at 237.711 kb on - strand at 237.711 kb on - strand at 237.711 kb on - strand at 237.722 kb on + strand at 237.722 kb on + strand at 237.723 kb on - strand at 237.723 kb on - strand at 237.742 kb on + strand at 237.742 kb on + strand at 237.742 kb on + strand at 237.743 kb on - strand at 237.743 kb on - strand at 237.762 kb on - strand at 237.842 kb on + strand at 237.842 kb on + strand, within Pf1N1B4_5490 at 237.842 kb on + strand, within Pf1N1B4_5490 at 237.843 kb on - strand, within Pf1N1B4_5490 at 237.843 kb on - strand, within Pf1N1B4_5490 at 237.843 kb on - strand, within Pf1N1B4_5490 at 237.848 kb on + strand, within Pf1N1B4_5490 at 237.848 kb on + strand, within Pf1N1B4_5490 at 237.848 kb on + strand, within Pf1N1B4_5490 at 237.849 kb on - strand, within Pf1N1B4_5490 at 237.849 kb on - strand, within Pf1N1B4_5490 at 237.854 kb on + strand at 237.855 kb on - strand, within Pf1N1B4_5490 at 237.896 kb on + strand, within Pf1N1B4_5490 at 237.896 kb on + strand, within Pf1N1B4_5490 at 237.908 kb on + strand, within Pf1N1B4_5490 at 237.909 kb on - strand, within Pf1N1B4_5490 at 237.909 kb on - strand, within Pf1N1B4_5490 at 237.942 kb on + strand, within Pf1N1B4_5490 at 237.943 kb on - strand, within Pf1N1B4_5490 at 238.020 kb on + strand at 238.020 kb on + strand at 238.020 kb on + strand at 238.020 kb on + strand at 238.020 kb on + strand at 238.021 kb on - strand at 238.036 kb on + strand at 238.036 kb on + strand at 238.036 kb on + strand at 238.036 kb on + strand at 238.036 kb on + strand at 238.074 kb on + strand at 238.074 kb on + strand at 238.074 kb on + strand at 238.075 kb on - strand at 238.075 kb on - strand at 238.075 kb on - strand at 238.105 kb on + strand at 238.171 kb on + strand at 238.234 kb on + strand at 238.281 kb on + strand at 238.281 kb on + strand at 238.281 kb on + strand at 238.281 kb on + strand at 238.282 kb on - strand at 238.282 kb on - strand at 238.282 kb on - strand at 238.332 kb on + strand at 238.332 kb on + strand at 238.333 kb on - strand at 238.333 kb on - strand at 238.346 kb on + strand at 238.618 kb on + strand at 238.619 kb on - strand at 238.619 kb on - strand at 238.828 kb on + strand, within Pf1N1B4_5492 at 238.828 kb on + strand, within Pf1N1B4_5492 at 238.828 kb on + strand, within Pf1N1B4_5492 at 238.829 kb on - strand at 238.832 kb on + strand, within Pf1N1B4_5492 at 238.832 kb on + strand, within Pf1N1B4_5492 at 238.832 kb on + strand, within Pf1N1B4_5492 at 238.832 kb on + strand at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.833 kb on - strand at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.833 kb on - strand, within Pf1N1B4_5492 at 238.932 kb on + strand at 238.932 kb on + strand at 238.932 kb on + strand at 238.950 kb on + strand at 238.950 kb on + strand at 238.950 kb on + strand at 238.950 kb on + strand at 238.951 kb on - strand at 238.951 kb on - strand at 238.951 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 236,914 + -0.0 236,914 + +2.5 236,914 + +1.3 236,915 - -1.1 236,915 - +0.2 236,925 + -0.5 236,925 + +1.0 236,926 - -0.6 236,926 - -2.2 236,926 - -0.4 236,965 + -0.5 236,970 + +0.9 237,092 + +0.2 237,092 + +2.0 237,111 - +0.8 237,111 - +2.2 237,135 + +0.6 237,136 - -1.7 237,136 - -0.1 237,139 + -2.5 237,140 - -1.4 237,140 - -0.1 237,140 - -0.7 237,175 - +0.2 237,256 + Pf1N1B4_5489 0.35 -0.0 237,256 + Pf1N1B4_5489 0.35 -0.4 237,257 - Pf1N1B4_5489 0.36 -0.4 237,257 - Pf1N1B4_5489 0.36 -0.2 237,257 - Pf1N1B4_5489 0.36 +0.5 237,257 - Pf1N1B4_5489 0.36 -0.4 237,257 - Pf1N1B4_5489 0.36 +0.1 237,257 - Pf1N1B4_5489 0.36 +0.1 237,257 - Pf1N1B4_5489 0.36 -2.2 237,480 - +0.7 237,480 - -0.2 237,480 - -0.2 237,480 - -0.5 237,522 - +0.4 237,531 + +0.9 237,538 + -0.1 237,538 + -0.3 237,538 + -0.3 237,538 + -1.6 237,538 + +0.4 237,539 - -2.4 237,539 - +1.2 237,539 - -1.1 237,539 - -0.0 237,539 - -0.6 237,539 - -0.6 237,539 - -0.8 237,539 - +0.4 237,539 - +1.2 237,548 - -0.1 237,548 - +0.6 237,548 - +0.4 237,647 - -0.4 237,663 + -1.3 237,663 + +0.6 237,663 + -3.2 237,663 + -1.7 237,664 - +1.2 237,664 - -1.2 237,664 - -0.1 237,664 - -1.9 237,710 + +1.4 237,710 + -2.1 237,710 + +1.8 237,711 - -0.1 237,711 - -0.2 237,711 - +1.4 237,711 - +0.6 237,722 + +1.2 237,722 + -0.6 237,723 - +0.1 237,723 - +1.2 237,742 + +2.0 237,742 + -2.0 237,742 + +0.7 237,743 - -0.5 237,743 - +0.7 237,762 - -0.2 237,842 + +1.2 237,842 + Pf1N1B4_5490 0.16 -0.6 237,842 + Pf1N1B4_5490 0.16 -1.8 237,843 - Pf1N1B4_5490 0.16 +2.5 237,843 - Pf1N1B4_5490 0.16 +1.0 237,843 - Pf1N1B4_5490 0.16 -0.0 237,848 + Pf1N1B4_5490 0.18 -0.9 237,848 + Pf1N1B4_5490 0.18 -1.9 237,848 + Pf1N1B4_5490 0.18 -0.2 237,849 - Pf1N1B4_5490 0.19 -0.3 237,849 - Pf1N1B4_5490 0.19 +1.2 237,854 + +2.2 237,855 - Pf1N1B4_5490 0.21 +0.2 237,896 + Pf1N1B4_5490 0.39 +2.2 237,896 + Pf1N1B4_5490 0.39 +2.4 237,908 + Pf1N1B4_5490 0.44 -0.1 237,909 - Pf1N1B4_5490 0.44 +1.0 237,909 - Pf1N1B4_5490 0.44 +1.1 237,942 + Pf1N1B4_5490 0.59 -0.1 237,943 - Pf1N1B4_5490 0.59 +0.4 238,020 + -0.2 238,020 + +1.6 238,020 + +1.2 238,020 + +1.2 238,020 + +0.6 238,021 - +0.5 238,036 + -1.4 238,036 + -0.1 238,036 + +0.4 238,036 + +0.8 238,036 + -0.2 238,074 + -0.7 238,074 + +0.6 238,074 + -0.7 238,075 - +0.1 238,075 - -2.0 238,075 - +0.1 238,105 + +1.0 238,171 + -1.1 238,234 + +0.3 238,281 + +1.1 238,281 + +0.2 238,281 + -0.7 238,281 + -1.7 238,282 - -1.4 238,282 - -1.6 238,282 - +0.5 238,332 + +0.9 238,332 + -1.5 238,333 - +0.2 238,333 - -0.3 238,346 + +0.4 238,618 + +1.4 238,619 - -0.7 238,619 - -0.8 238,828 + Pf1N1B4_5492 0.79 -1.8 238,828 + Pf1N1B4_5492 0.79 -0.2 238,828 + Pf1N1B4_5492 0.79 -1.0 238,829 - +0.8 238,832 + Pf1N1B4_5492 0.81 -0.6 238,832 + Pf1N1B4_5492 0.81 -2.0 238,832 + Pf1N1B4_5492 0.81 +1.2 238,832 + +1.3 238,833 - Pf1N1B4_5492 0.82 +1.8 238,833 - Pf1N1B4_5492 0.82 +0.2 238,833 - -0.5 238,833 - Pf1N1B4_5492 0.82 -1.2 238,833 - Pf1N1B4_5492 0.82 +0.9 238,833 - Pf1N1B4_5492 0.82 +1.4 238,932 + -0.2 238,932 + -2.0 238,932 + -1.1 238,950 + -0.2 238,950 + -0.8 238,950 + +1.2 238,950 + -0.1 238,951 - -0.3 238,951 - +1.2 238,951 - -1.7
Or see this region's nucleotide sequence