Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4996 and Pf1N1B4_4997 are separated by 40 nucleotides Pf1N1B4_4997 and Pf1N1B4_4998 are separated by 340 nucleotides
Pf1N1B4_4996 - Aspartate aminotransferase (EC 2.6.1.1), at 5,543,411 to 5,544,598
_4996
Pf1N1B4_4997 - Methyl-accepting chemotaxis protein, at 5,544,639 to 5,546,264
_4997
Pf1N1B4_4998 - Glycerate kinase (EC 2.7.1.31), at 5,546,605 to 5,547,747
_4998
Position (kb) 5544
5545
5546
5547 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5543.759 kb on + strand, within Pf1N1B4_4996 at 5543.979 kb on + strand, within Pf1N1B4_4996 at 5544.213 kb on + strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.283 kb on - strand, within Pf1N1B4_4996 at 5544.370 kb on + strand, within Pf1N1B4_4996 at 5544.371 kb on - strand, within Pf1N1B4_4996 at 5544.371 kb on - strand at 5544.396 kb on - strand, within Pf1N1B4_4996 at 5544.600 kb on + strand at 5544.600 kb on + strand at 5544.600 kb on + strand at 5544.636 kb on - strand at 5545.887 kb on + strand, within Pf1N1B4_4997 at 5545.888 kb on - strand, within Pf1N1B4_4997 at 5545.891 kb on + strand, within Pf1N1B4_4997 at 5545.891 kb on + strand, within Pf1N1B4_4997 at 5545.891 kb on + strand, within Pf1N1B4_4997 at 5545.891 kb on + strand, within Pf1N1B4_4997 at 5545.891 kb on + strand, within Pf1N1B4_4997 at 5545.891 kb on + strand, within Pf1N1B4_4997 at 5545.891 kb on + strand, within Pf1N1B4_4997 at 5545.891 kb on + strand at 5545.891 kb on + strand, within Pf1N1B4_4997 at 5545.892 kb on - strand, within Pf1N1B4_4997 at 5545.892 kb on - strand, within Pf1N1B4_4997 at 5546.049 kb on + strand, within Pf1N1B4_4997 at 5546.208 kb on + strand at 5546.252 kb on + strand at 5546.253 kb on - strand at 5546.264 kb on + strand at 5546.264 kb on + strand at 5546.264 kb on + strand at 5546.264 kb on + strand at 5546.264 kb on + strand at 5546.265 kb on - strand at 5546.265 kb on - strand at 5546.333 kb on + strand at 5546.359 kb on + strand at 5546.359 kb on + strand at 5546.359 kb on + strand at 5546.359 kb on + strand at 5546.360 kb on - strand at 5546.382 kb on - strand at 5546.382 kb on - strand at 5546.382 kb on - strand at 5546.427 kb on + strand at 5546.427 kb on + strand at 5546.477 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 5,543,759 + Pf1N1B4_4996 0.29 -1.4 5,543,979 + Pf1N1B4_4996 0.48 -0.0 5,544,213 + Pf1N1B4_4996 0.68 +0.3 5,544,214 - Pf1N1B4_4996 0.68 -2.4 5,544,214 - Pf1N1B4_4996 0.68 +0.1 5,544,214 - Pf1N1B4_4996 0.68 -0.0 5,544,214 - Pf1N1B4_4996 0.68 +0.4 5,544,222 + Pf1N1B4_4996 0.68 +0.1 5,544,222 + Pf1N1B4_4996 0.68 +0.6 5,544,222 + Pf1N1B4_4996 0.68 -0.2 5,544,222 + Pf1N1B4_4996 0.68 +0.3 5,544,222 + Pf1N1B4_4996 0.68 +0.2 5,544,223 - Pf1N1B4_4996 0.68 -1.0 5,544,223 - Pf1N1B4_4996 0.68 -1.2 5,544,223 - Pf1N1B4_4996 0.68 +1.5 5,544,223 - Pf1N1B4_4996 0.68 -0.4 5,544,283 - Pf1N1B4_4996 0.73 -0.0 5,544,370 + Pf1N1B4_4996 0.81 +1.2 5,544,371 - Pf1N1B4_4996 0.81 +0.3 5,544,371 - -0.1 5,544,396 - Pf1N1B4_4996 0.83 -1.0 5,544,600 + -0.4 5,544,600 + -2.5 5,544,600 + -0.2 5,544,636 - -1.5 5,545,887 + Pf1N1B4_4997 0.77 +1.5 5,545,888 - Pf1N1B4_4997 0.77 -0.8 5,545,891 + Pf1N1B4_4997 0.77 +0.2 5,545,891 + Pf1N1B4_4997 0.77 +0.5 5,545,891 + Pf1N1B4_4997 0.77 +1.8 5,545,891 + Pf1N1B4_4997 0.77 +0.5 5,545,891 + Pf1N1B4_4997 0.77 +1.0 5,545,891 + Pf1N1B4_4997 0.77 +1.4 5,545,891 + Pf1N1B4_4997 0.77 -1.2 5,545,891 + -0.7 5,545,891 + Pf1N1B4_4997 0.77 -0.7 5,545,892 - Pf1N1B4_4997 0.77 +0.5 5,545,892 - Pf1N1B4_4997 0.77 -0.7 5,546,049 + Pf1N1B4_4997 0.87 -0.0 5,546,208 + +1.5 5,546,252 + +0.7 5,546,253 - +0.3 5,546,264 + +1.1 5,546,264 + -0.5 5,546,264 + -1.2 5,546,264 + +0.3 5,546,264 + +1.5 5,546,265 - -0.7 5,546,265 - +0.8 5,546,333 + -0.7 5,546,359 + +1.1 5,546,359 + +0.3 5,546,359 + -0.1 5,546,359 + +0.2 5,546,360 - +1.4 5,546,382 - -0.2 5,546,382 - +0.4 5,546,382 - -0.7 5,546,427 + -0.2 5,546,427 + +0.4 5,546,477 + -0.2
Or see this region's nucleotide sequence