Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4846

Experiment: nitrogen source Ammonium chloride 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4845 and Pf1N1B4_4846 are separated by 126 nucleotidesPf1N1B4_4846 and Pf1N1B4_4847 are separated by 14 nucleotides Pf1N1B4_4845 - Xylulose kinase (EC 2.7.1.17), at 5,352,753 to 5,354,249 _4845 Pf1N1B4_4846 - Multiple polyol-specific dehydrogenase (EC 1.1.1.-), at 5,354,376 to 5,355,851 _4846 Pf1N1B4_4847 - Various polyols ABC transporter, ATP-binding component, at 5,355,866 to 5,356,969 _4847 Position (kb) 5354 5355 5356Strain fitness (log2 ratio) -2 -1 0 1at 5353.426 kb on + strand, within Pf1N1B4_4845at 5353.427 kb on - strandat 5353.473 kb on + strand, within Pf1N1B4_4845at 5353.473 kb on + strandat 5353.474 kb on - strand, within Pf1N1B4_4845at 5353.475 kb on + strand, within Pf1N1B4_4845at 5353.714 kb on + strand, within Pf1N1B4_4845at 5353.714 kb on + strand, within Pf1N1B4_4845at 5353.744 kb on + strand, within Pf1N1B4_4845at 5353.744 kb on + strand, within Pf1N1B4_4845at 5353.745 kb on - strandat 5353.745 kb on - strand, within Pf1N1B4_4845at 5353.753 kb on + strand, within Pf1N1B4_4845at 5354.037 kb on + strand, within Pf1N1B4_4845at 5354.038 kb on - strand, within Pf1N1B4_4845at 5354.232 kb on + strandat 5354.232 kb on + strandat 5354.232 kb on + strandat 5354.233 kb on - strandat 5354.233 kb on - strandat 5354.261 kb on - strandat 5354.581 kb on + strand, within Pf1N1B4_4846at 5354.582 kb on - strand, within Pf1N1B4_4846at 5354.582 kb on - strand, within Pf1N1B4_4846at 5354.582 kb on - strand, within Pf1N1B4_4846at 5354.792 kb on - strand, within Pf1N1B4_4846at 5354.851 kb on + strand, within Pf1N1B4_4846at 5354.863 kb on + strand, within Pf1N1B4_4846at 5354.864 kb on - strand, within Pf1N1B4_4846at 5354.929 kb on + strandat 5354.930 kb on - strand, within Pf1N1B4_4846at 5355.610 kb on + strand, within Pf1N1B4_4846at 5355.610 kb on + strand, within Pf1N1B4_4846at 5355.718 kb on + strandat 5355.719 kb on - strandat 5355.794 kb on - strandat 5356.404 kb on + strand, within Pf1N1B4_4847at 5356.404 kb on + strand, within Pf1N1B4_4847at 5356.404 kb on + strand, within Pf1N1B4_4847at 5356.404 kb on + strand, within Pf1N1B4_4847at 5356.405 kb on - strand, within Pf1N1B4_4847at 5356.720 kb on - strand, within Pf1N1B4_4847at 5356.720 kb on - strand, within Pf1N1B4_4847

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM
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5,353,426 + Pf1N1B4_4845 0.45 +0.2
5,353,427 - +1.2
5,353,473 + Pf1N1B4_4845 0.48 -0.0
5,353,473 + -1.4
5,353,474 - Pf1N1B4_4845 0.48 -0.8
5,353,475 + Pf1N1B4_4845 0.48 +0.0
5,353,714 + Pf1N1B4_4845 0.64 -0.2
5,353,714 + Pf1N1B4_4845 0.64 -1.6
5,353,744 + Pf1N1B4_4845 0.66 -0.6
5,353,744 + Pf1N1B4_4845 0.66 -1.6
5,353,745 - -0.6
5,353,745 - Pf1N1B4_4845 0.66 +0.5
5,353,753 + Pf1N1B4_4845 0.67 -0.2
5,354,037 + Pf1N1B4_4845 0.86 -0.4
5,354,038 - Pf1N1B4_4845 0.86 -0.0
5,354,232 + -0.4
5,354,232 + -0.3
5,354,232 + -0.6
5,354,233 - -2.1
5,354,233 - -0.4
5,354,261 - -0.0
5,354,581 + Pf1N1B4_4846 0.14 +0.9
5,354,582 - Pf1N1B4_4846 0.14 +1.1
5,354,582 - Pf1N1B4_4846 0.14 +0.5
5,354,582 - Pf1N1B4_4846 0.14 -0.0
5,354,792 - Pf1N1B4_4846 0.28 -0.4
5,354,851 + Pf1N1B4_4846 0.32 -0.1
5,354,863 + Pf1N1B4_4846 0.33 -1.2
5,354,864 - Pf1N1B4_4846 0.33 +0.2
5,354,929 + -0.9
5,354,930 - Pf1N1B4_4846 0.38 +0.7
5,355,610 + Pf1N1B4_4846 0.84 +0.3
5,355,610 + Pf1N1B4_4846 0.84 +0.5
5,355,718 + +0.4
5,355,719 - -0.1
5,355,794 - -0.6
5,356,404 + Pf1N1B4_4847 0.49 -0.5
5,356,404 + Pf1N1B4_4847 0.49 +0.9
5,356,404 + Pf1N1B4_4847 0.49 +0.1
5,356,404 + Pf1N1B4_4847 0.49 +1.2
5,356,405 - Pf1N1B4_4847 0.49 -0.6
5,356,720 - Pf1N1B4_4847 0.77 -1.4
5,356,720 - Pf1N1B4_4847 0.77 +0.8

Or see this region's nucleotide sequence