Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3661

Experiment: nitrogen source Ammonium chloride 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3660 and Pf1N1B4_3661 are separated by 128 nucleotidesPf1N1B4_3661 and Pf1N1B4_3662 are separated by 6 nucleotidesPf1N1B4_3662 and Pf1N1B4_3663 overlap by 4 nucleotides Pf1N1B4_3660 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 4,065,636 to 4,066,418 _3660 Pf1N1B4_3661 - Glyoxylate carboligase (EC 4.1.1.47), at 4,066,547 to 4,068,322 _3661 Pf1N1B4_3662 - hypothetical protein, at 4,068,329 to 4,068,592 _3662 Pf1N1B4_3663 - GlcG protein, at 4,068,589 to 4,068,849 _3663 Position (kb) 4066 4067 4068 4069Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4065.570 kb on + strandat 4065.647 kb on + strandat 4065.698 kb on - strandat 4065.698 kb on - strandat 4065.698 kb on - strandat 4065.880 kb on + strand, within Pf1N1B4_3660at 4065.880 kb on + strand, within Pf1N1B4_3660at 4065.881 kb on - strand, within Pf1N1B4_3660at 4065.881 kb on - strand, within Pf1N1B4_3660at 4065.881 kb on - strand, within Pf1N1B4_3660at 4065.881 kb on - strand, within Pf1N1B4_3660at 4065.881 kb on - strand, within Pf1N1B4_3660at 4065.881 kb on - strand, within Pf1N1B4_3660at 4065.889 kb on + strand, within Pf1N1B4_3660at 4065.958 kb on + strand, within Pf1N1B4_3660at 4066.036 kb on + strand, within Pf1N1B4_3660at 4066.037 kb on - strand, within Pf1N1B4_3660at 4066.306 kb on + strand, within Pf1N1B4_3660at 4066.306 kb on + strand, within Pf1N1B4_3660at 4066.318 kb on + strand, within Pf1N1B4_3660at 4066.318 kb on + strand, within Pf1N1B4_3660at 4066.440 kb on - strandat 4066.440 kb on - strandat 4066.854 kb on + strand, within Pf1N1B4_3661at 4066.854 kb on + strand, within Pf1N1B4_3661at 4066.854 kb on + strand, within Pf1N1B4_3661at 4066.926 kb on + strand, within Pf1N1B4_3661at 4066.926 kb on + strand, within Pf1N1B4_3661at 4066.927 kb on - strand, within Pf1N1B4_3661at 4066.927 kb on - strand, within Pf1N1B4_3661at 4066.927 kb on - strand, within Pf1N1B4_3661at 4066.983 kb on + strand, within Pf1N1B4_3661at 4066.983 kb on + strand, within Pf1N1B4_3661at 4066.984 kb on - strand, within Pf1N1B4_3661at 4066.984 kb on - strand, within Pf1N1B4_3661at 4066.984 kb on - strandat 4066.984 kb on - strand, within Pf1N1B4_3661at 4067.103 kb on + strand, within Pf1N1B4_3661at 4067.104 kb on - strand, within Pf1N1B4_3661at 4067.104 kb on - strand, within Pf1N1B4_3661at 4067.157 kb on + strand, within Pf1N1B4_3661at 4067.157 kb on + strand, within Pf1N1B4_3661at 4067.158 kb on - strand, within Pf1N1B4_3661at 4067.158 kb on - strand, within Pf1N1B4_3661at 4067.158 kb on - strand, within Pf1N1B4_3661at 4067.197 kb on - strand, within Pf1N1B4_3661at 4067.197 kb on - strand, within Pf1N1B4_3661at 4067.219 kb on + strand, within Pf1N1B4_3661at 4067.449 kb on - strand, within Pf1N1B4_3661at 4067.790 kb on + strand, within Pf1N1B4_3661at 4067.791 kb on - strand, within Pf1N1B4_3661at 4067.791 kb on - strand, within Pf1N1B4_3661at 4067.791 kb on - strand, within Pf1N1B4_3661at 4067.880 kb on + strand, within Pf1N1B4_3661at 4067.881 kb on - strand, within Pf1N1B4_3661at 4067.881 kb on - strand, within Pf1N1B4_3661at 4067.881 kb on - strand, within Pf1N1B4_3661at 4067.881 kb on - strand, within Pf1N1B4_3661at 4067.881 kb on - strand, within Pf1N1B4_3661at 4067.958 kb on - strand, within Pf1N1B4_3661at 4067.958 kb on - strand, within Pf1N1B4_3661at 4067.958 kb on - strand, within Pf1N1B4_3661at 4068.061 kb on - strandat 4068.222 kb on + strandat 4068.353 kb on - strandat 4068.355 kb on - strandat 4068.377 kb on + strand, within Pf1N1B4_3662at 4068.377 kb on + strand, within Pf1N1B4_3662at 4068.377 kb on + strand, within Pf1N1B4_3662at 4068.377 kb on + strand, within Pf1N1B4_3662at 4068.378 kb on - strand, within Pf1N1B4_3662at 4068.378 kb on - strand, within Pf1N1B4_3662at 4068.378 kb on - strand, within Pf1N1B4_3662at 4068.378 kb on - strand, within Pf1N1B4_3662at 4068.378 kb on - strand, within Pf1N1B4_3662at 4068.378 kb on - strand, within Pf1N1B4_3662at 4068.378 kb on - strand, within Pf1N1B4_3662at 4068.378 kb on - strand, within Pf1N1B4_3662at 4068.599 kb on + strandat 4068.707 kb on + strand, within Pf1N1B4_3663at 4069.096 kb on - strandat 4069.129 kb on - strandat 4069.177 kb on + strandat 4069.178 kb on - strandat 4069.178 kb on - strandat 4069.178 kb on - strandat 4069.178 kb on - strandat 4069.178 kb on - strandat 4069.178 kb on - strandat 4069.178 kb on - strandat 4069.178 kb on - strandat 4069.202 kb on + strandat 4069.203 kb on - strandat 4069.250 kb on + strandat 4069.251 kb on - strandat 4069.251 kb on - strandat 4069.269 kb on - strandat 4069.303 kb on + strandat 4069.314 kb on + strandat 4069.315 kb on - strandat 4069.315 kb on - strandat 4069.315 kb on - strandat 4069.320 kb on + strandat 4069.320 kb on + strandat 4069.321 kb on - strandat 4069.321 kb on - strandat 4069.321 kb on - strandat 4069.321 kb on - strandat 4069.321 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM
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4,065,570 + +0.4
4,065,647 + -1.0
4,065,698 - -1.2
4,065,698 - -0.7
4,065,698 - -1.0
4,065,880 + Pf1N1B4_3660 0.31 +0.6
4,065,880 + Pf1N1B4_3660 0.31 -0.5
4,065,881 - Pf1N1B4_3660 0.31 -0.2
4,065,881 - Pf1N1B4_3660 0.31 +1.3
4,065,881 - Pf1N1B4_3660 0.31 +0.3
4,065,881 - Pf1N1B4_3660 0.31 +0.1
4,065,881 - Pf1N1B4_3660 0.31 +1.1
4,065,881 - Pf1N1B4_3660 0.31 +0.2
4,065,889 + Pf1N1B4_3660 0.32 +0.5
4,065,958 + Pf1N1B4_3660 0.41 -0.2
4,066,036 + Pf1N1B4_3660 0.51 +0.1
4,066,037 - Pf1N1B4_3660 0.51 -0.3
4,066,306 + Pf1N1B4_3660 0.86 -3.3
4,066,306 + Pf1N1B4_3660 0.86 -0.4
4,066,318 + Pf1N1B4_3660 0.87 +0.7
4,066,318 + Pf1N1B4_3660 0.87 +0.2
4,066,440 - +0.2
4,066,440 - -0.3
4,066,854 + Pf1N1B4_3661 0.17 +1.1
4,066,854 + Pf1N1B4_3661 0.17 +0.7
4,066,854 + Pf1N1B4_3661 0.17 -1.7
4,066,926 + Pf1N1B4_3661 0.21 +0.7
4,066,926 + Pf1N1B4_3661 0.21 -0.2
4,066,927 - Pf1N1B4_3661 0.21 -0.3
4,066,927 - Pf1N1B4_3661 0.21 -1.8
4,066,927 - Pf1N1B4_3661 0.21 -2.4
4,066,983 + Pf1N1B4_3661 0.25 +0.2
4,066,983 + Pf1N1B4_3661 0.25 +0.1
4,066,984 - Pf1N1B4_3661 0.25 -0.2
4,066,984 - Pf1N1B4_3661 0.25 -1.4
4,066,984 - -0.2
4,066,984 - Pf1N1B4_3661 0.25 +0.4
4,067,103 + Pf1N1B4_3661 0.31 -0.7
4,067,104 - Pf1N1B4_3661 0.31 -0.9
4,067,104 - Pf1N1B4_3661 0.31 -0.4
4,067,157 + Pf1N1B4_3661 0.34 -1.5
4,067,157 + Pf1N1B4_3661 0.34 -1.9
4,067,158 - Pf1N1B4_3661 0.34 +0.4
4,067,158 - Pf1N1B4_3661 0.34 -0.4
4,067,158 - Pf1N1B4_3661 0.34 -0.7
4,067,197 - Pf1N1B4_3661 0.37 +0.1
4,067,197 - Pf1N1B4_3661 0.37 -2.5
4,067,219 + Pf1N1B4_3661 0.38 -0.0
4,067,449 - Pf1N1B4_3661 0.51 +2.4
4,067,790 + Pf1N1B4_3661 0.70 -0.3
4,067,791 - Pf1N1B4_3661 0.70 +0.1
4,067,791 - Pf1N1B4_3661 0.70 +1.1
4,067,791 - Pf1N1B4_3661 0.70 -0.9
4,067,880 + Pf1N1B4_3661 0.75 -1.8
4,067,881 - Pf1N1B4_3661 0.75 -0.0
4,067,881 - Pf1N1B4_3661 0.75 +0.3
4,067,881 - Pf1N1B4_3661 0.75 -0.3
4,067,881 - Pf1N1B4_3661 0.75 -0.3
4,067,881 - Pf1N1B4_3661 0.75 -0.5
4,067,958 - Pf1N1B4_3661 0.79 -1.3
4,067,958 - Pf1N1B4_3661 0.79 -2.7
4,067,958 - Pf1N1B4_3661 0.79 -1.1
4,068,061 - -2.1
4,068,222 + -0.8
4,068,353 - +0.1
4,068,355 - +1.2
4,068,377 + Pf1N1B4_3662 0.18 -1.0
4,068,377 + Pf1N1B4_3662 0.18 -0.6
4,068,377 + Pf1N1B4_3662 0.18 -2.4
4,068,377 + Pf1N1B4_3662 0.18 +0.2
4,068,378 - Pf1N1B4_3662 0.19 -0.6
4,068,378 - Pf1N1B4_3662 0.19 +1.2
4,068,378 - Pf1N1B4_3662 0.19 +0.6
4,068,378 - Pf1N1B4_3662 0.19 -0.5
4,068,378 - Pf1N1B4_3662 0.19 -0.6
4,068,378 - Pf1N1B4_3662 0.19 +0.6
4,068,378 - Pf1N1B4_3662 0.19 -0.3
4,068,378 - Pf1N1B4_3662 0.19 -0.8
4,068,599 + +0.2
4,068,707 + Pf1N1B4_3663 0.45 +0.4
4,069,096 - -0.5
4,069,129 - +0.1
4,069,177 + +2.4
4,069,178 - +1.0
4,069,178 - -1.0
4,069,178 - -1.1
4,069,178 - +0.0
4,069,178 - +0.5
4,069,178 - +1.8
4,069,178 - +1.1
4,069,178 - -2.4
4,069,202 + -0.1
4,069,203 - -0.3
4,069,250 + +0.4
4,069,251 - -2.2
4,069,251 - -1.8
4,069,269 - +1.3
4,069,303 + +0.6
4,069,314 + +1.5
4,069,315 - -1.1
4,069,315 - -0.5
4,069,315 - +0.7
4,069,320 + -2.3
4,069,320 + -0.2
4,069,321 - -0.0
4,069,321 - -1.6
4,069,321 - -0.3
4,069,321 - +0.9
4,069,321 - -0.5

Or see this region's nucleotide sequence