Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3660 and Pf1N1B4_3661 are separated by 128 nucleotides Pf1N1B4_3661 and Pf1N1B4_3662 are separated by 6 nucleotides Pf1N1B4_3662 and Pf1N1B4_3663 overlap by 4 nucleotides
Pf1N1B4_3660 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 4,065,636 to 4,066,418
_3660
Pf1N1B4_3661 - Glyoxylate carboligase (EC 4.1.1.47), at 4,066,547 to 4,068,322
_3661
Pf1N1B4_3662 - hypothetical protein, at 4,068,329 to 4,068,592
_3662
Pf1N1B4_3663 - GlcG protein, at 4,068,589 to 4,068,849
_3663
Position (kb)
4066
4067
4068
4069 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4065.570 kb on + strand at 4065.647 kb on + strand at 4065.698 kb on - strand at 4065.698 kb on - strand at 4065.698 kb on - strand at 4065.880 kb on + strand, within Pf1N1B4_3660 at 4065.880 kb on + strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.889 kb on + strand, within Pf1N1B4_3660 at 4065.958 kb on + strand, within Pf1N1B4_3660 at 4066.036 kb on + strand, within Pf1N1B4_3660 at 4066.037 kb on - strand, within Pf1N1B4_3660 at 4066.306 kb on + strand, within Pf1N1B4_3660 at 4066.306 kb on + strand, within Pf1N1B4_3660 at 4066.318 kb on + strand, within Pf1N1B4_3660 at 4066.318 kb on + strand, within Pf1N1B4_3660 at 4066.440 kb on - strand at 4066.440 kb on - strand at 4066.854 kb on + strand, within Pf1N1B4_3661 at 4066.854 kb on + strand, within Pf1N1B4_3661 at 4066.854 kb on + strand, within Pf1N1B4_3661 at 4066.926 kb on + strand, within Pf1N1B4_3661 at 4066.926 kb on + strand, within Pf1N1B4_3661 at 4066.927 kb on - strand, within Pf1N1B4_3661 at 4066.927 kb on - strand, within Pf1N1B4_3661 at 4066.927 kb on - strand, within Pf1N1B4_3661 at 4066.983 kb on + strand, within Pf1N1B4_3661 at 4066.983 kb on + strand, within Pf1N1B4_3661 at 4066.984 kb on - strand, within Pf1N1B4_3661 at 4066.984 kb on - strand, within Pf1N1B4_3661 at 4066.984 kb on - strand at 4066.984 kb on - strand, within Pf1N1B4_3661 at 4067.103 kb on + strand, within Pf1N1B4_3661 at 4067.104 kb on - strand, within Pf1N1B4_3661 at 4067.104 kb on - strand, within Pf1N1B4_3661 at 4067.157 kb on + strand, within Pf1N1B4_3661 at 4067.157 kb on + strand, within Pf1N1B4_3661 at 4067.158 kb on - strand, within Pf1N1B4_3661 at 4067.158 kb on - strand, within Pf1N1B4_3661 at 4067.158 kb on - strand, within Pf1N1B4_3661 at 4067.197 kb on - strand, within Pf1N1B4_3661 at 4067.197 kb on - strand, within Pf1N1B4_3661 at 4067.219 kb on + strand, within Pf1N1B4_3661 at 4067.449 kb on - strand, within Pf1N1B4_3661 at 4067.790 kb on + strand, within Pf1N1B4_3661 at 4067.791 kb on - strand, within Pf1N1B4_3661 at 4067.791 kb on - strand, within Pf1N1B4_3661 at 4067.791 kb on - strand, within Pf1N1B4_3661 at 4067.880 kb on + strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.958 kb on - strand, within Pf1N1B4_3661 at 4067.958 kb on - strand, within Pf1N1B4_3661 at 4067.958 kb on - strand, within Pf1N1B4_3661 at 4068.061 kb on - strand at 4068.222 kb on + strand at 4068.353 kb on - strand at 4068.355 kb on - strand at 4068.377 kb on + strand, within Pf1N1B4_3662 at 4068.377 kb on + strand, within Pf1N1B4_3662 at 4068.377 kb on + strand, within Pf1N1B4_3662 at 4068.377 kb on + strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.599 kb on + strand at 4068.707 kb on + strand, within Pf1N1B4_3663 at 4069.096 kb on - strand at 4069.129 kb on - strand at 4069.177 kb on + strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.202 kb on + strand at 4069.203 kb on - strand at 4069.250 kb on + strand at 4069.251 kb on - strand at 4069.251 kb on - strand at 4069.269 kb on - strand at 4069.303 kb on + strand at 4069.314 kb on + strand at 4069.315 kb on - strand at 4069.315 kb on - strand at 4069.315 kb on - strand at 4069.320 kb on + strand at 4069.320 kb on + strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 4,065,570 + +0.4 4,065,647 + -1.0 4,065,698 - -1.2 4,065,698 - -0.7 4,065,698 - -1.0 4,065,880 + Pf1N1B4_3660 0.31 +0.6 4,065,880 + Pf1N1B4_3660 0.31 -0.5 4,065,881 - Pf1N1B4_3660 0.31 -0.2 4,065,881 - Pf1N1B4_3660 0.31 +1.3 4,065,881 - Pf1N1B4_3660 0.31 +0.3 4,065,881 - Pf1N1B4_3660 0.31 +0.1 4,065,881 - Pf1N1B4_3660 0.31 +1.1 4,065,881 - Pf1N1B4_3660 0.31 +0.2 4,065,889 + Pf1N1B4_3660 0.32 +0.5 4,065,958 + Pf1N1B4_3660 0.41 -0.2 4,066,036 + Pf1N1B4_3660 0.51 +0.1 4,066,037 - Pf1N1B4_3660 0.51 -0.3 4,066,306 + Pf1N1B4_3660 0.86 -3.3 4,066,306 + Pf1N1B4_3660 0.86 -0.4 4,066,318 + Pf1N1B4_3660 0.87 +0.7 4,066,318 + Pf1N1B4_3660 0.87 +0.2 4,066,440 - +0.2 4,066,440 - -0.3 4,066,854 + Pf1N1B4_3661 0.17 +1.1 4,066,854 + Pf1N1B4_3661 0.17 +0.7 4,066,854 + Pf1N1B4_3661 0.17 -1.7 4,066,926 + Pf1N1B4_3661 0.21 +0.7 4,066,926 + Pf1N1B4_3661 0.21 -0.2 4,066,927 - Pf1N1B4_3661 0.21 -0.3 4,066,927 - Pf1N1B4_3661 0.21 -1.8 4,066,927 - Pf1N1B4_3661 0.21 -2.4 4,066,983 + Pf1N1B4_3661 0.25 +0.2 4,066,983 + Pf1N1B4_3661 0.25 +0.1 4,066,984 - Pf1N1B4_3661 0.25 -0.2 4,066,984 - Pf1N1B4_3661 0.25 -1.4 4,066,984 - -0.2 4,066,984 - Pf1N1B4_3661 0.25 +0.4 4,067,103 + Pf1N1B4_3661 0.31 -0.7 4,067,104 - Pf1N1B4_3661 0.31 -0.9 4,067,104 - Pf1N1B4_3661 0.31 -0.4 4,067,157 + Pf1N1B4_3661 0.34 -1.5 4,067,157 + Pf1N1B4_3661 0.34 -1.9 4,067,158 - Pf1N1B4_3661 0.34 +0.4 4,067,158 - Pf1N1B4_3661 0.34 -0.4 4,067,158 - Pf1N1B4_3661 0.34 -0.7 4,067,197 - Pf1N1B4_3661 0.37 +0.1 4,067,197 - Pf1N1B4_3661 0.37 -2.5 4,067,219 + Pf1N1B4_3661 0.38 -0.0 4,067,449 - Pf1N1B4_3661 0.51 +2.4 4,067,790 + Pf1N1B4_3661 0.70 -0.3 4,067,791 - Pf1N1B4_3661 0.70 +0.1 4,067,791 - Pf1N1B4_3661 0.70 +1.1 4,067,791 - Pf1N1B4_3661 0.70 -0.9 4,067,880 + Pf1N1B4_3661 0.75 -1.8 4,067,881 - Pf1N1B4_3661 0.75 -0.0 4,067,881 - Pf1N1B4_3661 0.75 +0.3 4,067,881 - Pf1N1B4_3661 0.75 -0.3 4,067,881 - Pf1N1B4_3661 0.75 -0.3 4,067,881 - Pf1N1B4_3661 0.75 -0.5 4,067,958 - Pf1N1B4_3661 0.79 -1.3 4,067,958 - Pf1N1B4_3661 0.79 -2.7 4,067,958 - Pf1N1B4_3661 0.79 -1.1 4,068,061 - -2.1 4,068,222 + -0.8 4,068,353 - +0.1 4,068,355 - +1.2 4,068,377 + Pf1N1B4_3662 0.18 -1.0 4,068,377 + Pf1N1B4_3662 0.18 -0.6 4,068,377 + Pf1N1B4_3662 0.18 -2.4 4,068,377 + Pf1N1B4_3662 0.18 +0.2 4,068,378 - Pf1N1B4_3662 0.19 -0.6 4,068,378 - Pf1N1B4_3662 0.19 +1.2 4,068,378 - Pf1N1B4_3662 0.19 +0.6 4,068,378 - Pf1N1B4_3662 0.19 -0.5 4,068,378 - Pf1N1B4_3662 0.19 -0.6 4,068,378 - Pf1N1B4_3662 0.19 +0.6 4,068,378 - Pf1N1B4_3662 0.19 -0.3 4,068,378 - Pf1N1B4_3662 0.19 -0.8 4,068,599 + +0.2 4,068,707 + Pf1N1B4_3663 0.45 +0.4 4,069,096 - -0.5 4,069,129 - +0.1 4,069,177 + +2.4 4,069,178 - +1.0 4,069,178 - -1.0 4,069,178 - -1.1 4,069,178 - +0.0 4,069,178 - +0.5 4,069,178 - +1.8 4,069,178 - +1.1 4,069,178 - -2.4 4,069,202 + -0.1 4,069,203 - -0.3 4,069,250 + +0.4 4,069,251 - -2.2 4,069,251 - -1.8 4,069,269 - +1.3 4,069,303 + +0.6 4,069,314 + +1.5 4,069,315 - -1.1 4,069,315 - -0.5 4,069,315 - +0.7 4,069,320 + -2.3 4,069,320 + -0.2 4,069,321 - -0.0 4,069,321 - -1.6 4,069,321 - -0.3 4,069,321 - +0.9 4,069,321 - -0.5
Or see this region's nucleotide sequence