Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_362 and Pf1N1B4_363 overlap by 8 nucleotides Pf1N1B4_363 and Pf1N1B4_364 are separated by 3 nucleotides
Pf1N1B4_362 - Lipid A export ATP-binding/permease protein MsbA, at 430,596 to 432,413
_362
Pf1N1B4_363 - Thioredoxin, at 432,406 to 433,044
_363
Pf1N1B4_364 - Rhodanese domain protein UPF0176, at 433,048 to 433,989
_364
Position (kb) 432
433
434 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 431.426 kb on - strand, within Pf1N1B4_362 at 431.426 kb on - strand, within Pf1N1B4_362 at 431.624 kb on - strand, within Pf1N1B4_362 at 431.624 kb on - strand, within Pf1N1B4_362 at 431.803 kb on + strand, within Pf1N1B4_362 at 431.803 kb on + strand, within Pf1N1B4_362 at 431.803 kb on + strand, within Pf1N1B4_362 at 431.803 kb on + strand, within Pf1N1B4_362 at 431.804 kb on - strand, within Pf1N1B4_362 at 431.804 kb on - strand, within Pf1N1B4_362 at 432.154 kb on + strand at 432.471 kb on - strand, within Pf1N1B4_363 at 432.501 kb on + strand, within Pf1N1B4_363 at 432.502 kb on - strand, within Pf1N1B4_363 at 432.687 kb on - strand, within Pf1N1B4_363 at 432.687 kb on - strand, within Pf1N1B4_363 at 432.782 kb on + strand, within Pf1N1B4_363 at 432.865 kb on - strand, within Pf1N1B4_363 at 432.867 kb on - strand, within Pf1N1B4_363 at 432.867 kb on - strand, within Pf1N1B4_363 at 432.867 kb on - strand, within Pf1N1B4_363 at 432.937 kb on + strand, within Pf1N1B4_363 at 433.022 kb on + strand at 433.022 kb on + strand at 433.022 kb on + strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.073 kb on + strand at 433.074 kb on - strand at 433.074 kb on - strand at 433.074 kb on - strand at 433.074 kb on - strand at 433.074 kb on - strand at 433.079 kb on + strand at 433.079 kb on + strand at 433.080 kb on - strand at 433.205 kb on + strand, within Pf1N1B4_364 at 433.205 kb on + strand, within Pf1N1B4_364 at 433.206 kb on - strand, within Pf1N1B4_364 at 433.206 kb on - strand, within Pf1N1B4_364 at 433.206 kb on - strand, within Pf1N1B4_364 at 433.392 kb on - strand, within Pf1N1B4_364 at 433.421 kb on + strand, within Pf1N1B4_364 at 433.509 kb on - strand, within Pf1N1B4_364 at 433.652 kb on + strand, within Pf1N1B4_364 at 433.653 kb on - strand, within Pf1N1B4_364 at 433.653 kb on - strand, within Pf1N1B4_364 at 433.934 kb on + strand at 433.956 kb on + strand at 433.956 kb on + strand at 433.958 kb on + strand at 433.958 kb on + strand at 433.959 kb on - strand at 433.994 kb on - strand at 433.994 kb on - strand at 434.017 kb on + strand at 434.018 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 431,426 - Pf1N1B4_362 0.46 -0.4 431,426 - Pf1N1B4_362 0.46 -1.2 431,624 - Pf1N1B4_362 0.57 +1.2 431,624 - Pf1N1B4_362 0.57 +0.9 431,803 + Pf1N1B4_362 0.66 -1.0 431,803 + Pf1N1B4_362 0.66 +0.1 431,803 + Pf1N1B4_362 0.66 -0.4 431,803 + Pf1N1B4_362 0.66 -0.1 431,804 - Pf1N1B4_362 0.66 +0.8 431,804 - Pf1N1B4_362 0.66 +1.0 432,154 + -0.5 432,471 - Pf1N1B4_363 0.10 +1.4 432,501 + Pf1N1B4_363 0.15 -0.4 432,502 - Pf1N1B4_363 0.15 -1.0 432,687 - Pf1N1B4_363 0.44 +1.0 432,687 - Pf1N1B4_363 0.44 +0.4 432,782 + Pf1N1B4_363 0.59 +2.7 432,865 - Pf1N1B4_363 0.72 -0.9 432,867 - Pf1N1B4_363 0.72 -0.3 432,867 - Pf1N1B4_363 0.72 +1.0 432,867 - Pf1N1B4_363 0.72 +1.6 432,937 + Pf1N1B4_363 0.83 -0.4 433,022 + -0.4 433,022 + +0.7 433,022 + -0.7 433,023 - -0.1 433,023 - -0.1 433,023 - -3.0 433,023 - +0.3 433,023 - -1.6 433,023 - -0.7 433,073 + -0.5 433,074 - -1.6 433,074 - +1.4 433,074 - +1.9 433,074 - +1.0 433,074 - +1.3 433,079 + -0.9 433,079 + -1.9 433,080 - -3.4 433,205 + Pf1N1B4_364 0.17 -0.4 433,205 + Pf1N1B4_364 0.17 +0.8 433,206 - Pf1N1B4_364 0.17 -0.8 433,206 - Pf1N1B4_364 0.17 -1.1 433,206 - Pf1N1B4_364 0.17 +0.8 433,392 - Pf1N1B4_364 0.37 -0.7 433,421 + Pf1N1B4_364 0.40 -0.3 433,509 - Pf1N1B4_364 0.49 +0.1 433,652 + Pf1N1B4_364 0.64 +1.5 433,653 - Pf1N1B4_364 0.64 -1.2 433,653 - Pf1N1B4_364 0.64 +0.5 433,934 + -0.1 433,956 + +0.9 433,956 + -1.0 433,958 + -0.7 433,958 + +0.1 433,959 - +1.5 433,994 - -2.0 433,994 - -0.4 434,017 + +0.8 434,018 - -0.6
Or see this region's nucleotide sequence