Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3265 and Pf1N1B4_3266 are separated by 349 nucleotides Pf1N1B4_3266 and Pf1N1B4_3267 are separated by 14 nucleotides Pf1N1B4_3267 and Pf1N1B4_3268 are separated by 199 nucleotides Pf1N1B4_3268 and Pf1N1B4_3269 are separated by 192 nucleotides
Pf1N1B4_3265 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,656,022 to 3,656,942
_3265
Pf1N1B4_3266 - ThiJ/PfpI family protein, at 3,657,292 to 3,657,873
_3266
Pf1N1B4_3267 - FIG00954264: hypothetical protein, at 3,657,888 to 3,658,193
_3267
Pf1N1B4_3268 - FIG00955946: hypothetical protein, at 3,658,393 to 3,658,737
_3268
Pf1N1B4_3269 - FIG00953317: hypothetical protein, at 3,658,930 to 3,659,949
_3269
Position (kb)
3657
3658
3659 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3656.892 kb on - strand at 3656.981 kb on + strand at 3656.981 kb on + strand at 3656.981 kb on + strand at 3656.985 kb on + strand at 3656.985 kb on + strand at 3656.985 kb on + strand at 3656.986 kb on - strand at 3656.992 kb on - strand at 3657.014 kb on - strand at 3657.019 kb on + strand at 3657.020 kb on - strand at 3657.020 kb on - strand at 3657.020 kb on - strand at 3657.031 kb on + strand at 3657.037 kb on + strand at 3657.192 kb on + strand at 3657.206 kb on - strand at 3657.208 kb on + strand at 3657.209 kb on - strand at 3657.209 kb on - strand at 3657.219 kb on + strand at 3657.242 kb on + strand at 3657.242 kb on + strand at 3657.242 kb on + strand at 3657.242 kb on + strand at 3657.243 kb on - strand at 3657.243 kb on - strand at 3657.243 kb on - strand at 3657.251 kb on + strand at 3657.251 kb on + strand at 3657.251 kb on + strand at 3657.252 kb on - strand at 3657.252 kb on - strand at 3657.252 kb on - strand at 3657.252 kb on - strand at 3657.252 kb on - strand at 3657.252 kb on - strand at 3657.252 kb on - strand at 3657.252 kb on - strand at 3657.340 kb on + strand at 3657.341 kb on - strand at 3657.341 kb on - strand at 3657.341 kb on - strand at 3657.341 kb on - strand at 3657.389 kb on + strand, within Pf1N1B4_3266 at 3657.389 kb on + strand, within Pf1N1B4_3266 at 3657.389 kb on + strand, within Pf1N1B4_3266 at 3657.390 kb on - strand, within Pf1N1B4_3266 at 3657.390 kb on - strand, within Pf1N1B4_3266 at 3657.466 kb on + strand, within Pf1N1B4_3266 at 3657.466 kb on + strand, within Pf1N1B4_3266 at 3657.466 kb on + strand, within Pf1N1B4_3266 at 3657.466 kb on + strand, within Pf1N1B4_3266 at 3657.466 kb on + strand, within Pf1N1B4_3266 at 3657.467 kb on - strand, within Pf1N1B4_3266 at 3657.467 kb on - strand, within Pf1N1B4_3266 at 3657.467 kb on - strand, within Pf1N1B4_3266 at 3657.467 kb on - strand, within Pf1N1B4_3266 at 3657.518 kb on + strand, within Pf1N1B4_3266 at 3657.518 kb on + strand, within Pf1N1B4_3266 at 3657.518 kb on + strand, within Pf1N1B4_3266 at 3657.518 kb on + strand, within Pf1N1B4_3266 at 3657.518 kb on + strand, within Pf1N1B4_3266 at 3657.518 kb on + strand, within Pf1N1B4_3266 at 3657.518 kb on + strand, within Pf1N1B4_3266 at 3657.518 kb on + strand, within Pf1N1B4_3266 at 3657.518 kb on + strand, within Pf1N1B4_3266 at 3657.519 kb on - strand, within Pf1N1B4_3266 at 3657.519 kb on - strand, within Pf1N1B4_3266 at 3657.519 kb on - strand, within Pf1N1B4_3266 at 3657.519 kb on - strand, within Pf1N1B4_3266 at 3657.519 kb on - strand, within Pf1N1B4_3266 at 3657.519 kb on - strand, within Pf1N1B4_3266 at 3657.572 kb on - strand, within Pf1N1B4_3266 at 3657.572 kb on - strand, within Pf1N1B4_3266 at 3657.871 kb on + strand at 3657.900 kb on + strand at 3657.900 kb on + strand at 3657.901 kb on - strand at 3657.924 kb on + strand, within Pf1N1B4_3267 at 3657.924 kb on + strand, within Pf1N1B4_3267 at 3657.925 kb on - strand, within Pf1N1B4_3267 at 3658.059 kb on + strand, within Pf1N1B4_3267 at 3658.059 kb on + strand, within Pf1N1B4_3267 at 3658.060 kb on - strand, within Pf1N1B4_3267 at 3658.124 kb on + strand, within Pf1N1B4_3267 at 3658.124 kb on + strand, within Pf1N1B4_3267 at 3658.125 kb on - strand, within Pf1N1B4_3267 at 3658.125 kb on - strand, within Pf1N1B4_3267 at 3658.131 kb on + strand, within Pf1N1B4_3267 at 3658.132 kb on - strand, within Pf1N1B4_3267 at 3658.191 kb on + strand at 3658.191 kb on + strand at 3658.192 kb on - strand at 3658.236 kb on + strand at 3658.236 kb on + strand at 3658.290 kb on - strand at 3658.351 kb on - strand at 3658.386 kb on + strand at 3658.386 kb on + strand at 3658.386 kb on + strand at 3658.386 kb on + strand at 3658.386 kb on + strand at 3658.387 kb on - strand at 3658.387 kb on - strand at 3658.387 kb on - strand at 3658.387 kb on - strand at 3658.406 kb on - strand at 3658.406 kb on - strand at 3658.445 kb on + strand at 3658.446 kb on - strand, within Pf1N1B4_3268 at 3658.446 kb on - strand, within Pf1N1B4_3268 at 3658.489 kb on + strand, within Pf1N1B4_3268 at 3658.489 kb on + strand, within Pf1N1B4_3268 at 3658.489 kb on + strand, within Pf1N1B4_3268 at 3658.489 kb on + strand, within Pf1N1B4_3268 at 3658.489 kb on + strand, within Pf1N1B4_3268 at 3658.489 kb on + strand, within Pf1N1B4_3268 at 3658.489 kb on + strand, within Pf1N1B4_3268 at 3658.489 kb on + strand, within Pf1N1B4_3268 at 3658.490 kb on - strand, within Pf1N1B4_3268 at 3658.490 kb on - strand, within Pf1N1B4_3268 at 3658.490 kb on - strand, within Pf1N1B4_3268 at 3658.490 kb on - strand, within Pf1N1B4_3268 at 3658.490 kb on - strand, within Pf1N1B4_3268 at 3658.490 kb on - strand, within Pf1N1B4_3268 at 3658.490 kb on - strand, within Pf1N1B4_3268 at 3658.558 kb on + strand, within Pf1N1B4_3268 at 3658.591 kb on + strand, within Pf1N1B4_3268 at 3658.591 kb on + strand, within Pf1N1B4_3268 at 3658.591 kb on + strand, within Pf1N1B4_3268 at 3658.591 kb on + strand, within Pf1N1B4_3268 at 3658.592 kb on - strand, within Pf1N1B4_3268 at 3658.592 kb on - strand, within Pf1N1B4_3268 at 3658.592 kb on - strand, within Pf1N1B4_3268 at 3658.592 kb on - strand, within Pf1N1B4_3268 at 3658.592 kb on - strand, within Pf1N1B4_3268 at 3658.592 kb on - strand, within Pf1N1B4_3268 at 3658.642 kb on + strand, within Pf1N1B4_3268 at 3658.643 kb on - strand, within Pf1N1B4_3268 at 3658.643 kb on - strand, within Pf1N1B4_3268 at 3658.643 kb on - strand, within Pf1N1B4_3268 at 3658.665 kb on + strand, within Pf1N1B4_3268 at 3658.735 kb on + strand at 3658.735 kb on + strand at 3658.751 kb on + strand at 3658.752 kb on - strand at 3658.752 kb on - strand at 3658.752 kb on - strand at 3658.760 kb on + strand at 3658.760 kb on + strand at 3658.760 kb on + strand at 3658.760 kb on + strand at 3658.785 kb on + strand at 3658.785 kb on + strand at 3658.861 kb on + strand at 3658.862 kb on - strand at 3658.862 kb on - strand at 3658.862 kb on - strand at 3658.862 kb on - strand at 3658.862 kb on - strand at 3658.862 kb on - strand at 3658.862 kb on - strand at 3659.030 kb on + strand at 3659.031 kb on - strand at 3659.185 kb on + strand, within Pf1N1B4_3269
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 3,656,892 - +0.9 3,656,981 + -1.5 3,656,981 + -1.6 3,656,981 + -0.9 3,656,985 + -0.7 3,656,985 + +0.4 3,656,985 + -1.8 3,656,986 - -0.2 3,656,992 - +0.3 3,657,014 - +0.8 3,657,019 + +0.5 3,657,020 - -1.0 3,657,020 - +0.9 3,657,020 - +0.7 3,657,031 + +0.5 3,657,037 + -0.7 3,657,192 + -1.5 3,657,206 - -0.3 3,657,208 + +0.3 3,657,209 - -0.0 3,657,209 - -1.4 3,657,219 + +0.7 3,657,242 + +1.3 3,657,242 + +0.2 3,657,242 + +0.7 3,657,242 + -2.4 3,657,243 - -1.1 3,657,243 - -0.6 3,657,243 - +0.7 3,657,251 + -2.0 3,657,251 + +1.3 3,657,251 + -1.1 3,657,252 - -0.3 3,657,252 - +0.6 3,657,252 - -0.5 3,657,252 - +0.1 3,657,252 - -0.1 3,657,252 - -0.0 3,657,252 - +0.4 3,657,252 - +0.7 3,657,340 + +0.1 3,657,341 - +0.5 3,657,341 - -0.8 3,657,341 - +0.7 3,657,341 - +1.4 3,657,389 + Pf1N1B4_3266 0.17 +0.1 3,657,389 + Pf1N1B4_3266 0.17 +0.5 3,657,389 + Pf1N1B4_3266 0.17 -0.3 3,657,390 - Pf1N1B4_3266 0.17 +0.3 3,657,390 - Pf1N1B4_3266 0.17 -0.9 3,657,466 + Pf1N1B4_3266 0.30 -0.4 3,657,466 + Pf1N1B4_3266 0.30 +0.5 3,657,466 + Pf1N1B4_3266 0.30 -0.8 3,657,466 + Pf1N1B4_3266 0.30 +0.1 3,657,466 + Pf1N1B4_3266 0.30 -3.6 3,657,467 - Pf1N1B4_3266 0.30 +0.3 3,657,467 - Pf1N1B4_3266 0.30 -1.1 3,657,467 - Pf1N1B4_3266 0.30 -1.0 3,657,467 - Pf1N1B4_3266 0.30 -1.5 3,657,518 + Pf1N1B4_3266 0.39 -0.5 3,657,518 + Pf1N1B4_3266 0.39 +0.0 3,657,518 + Pf1N1B4_3266 0.39 +0.4 3,657,518 + Pf1N1B4_3266 0.39 +0.1 3,657,518 + Pf1N1B4_3266 0.39 -0.9 3,657,518 + Pf1N1B4_3266 0.39 -0.9 3,657,518 + Pf1N1B4_3266 0.39 -0.9 3,657,518 + Pf1N1B4_3266 0.39 -0.9 3,657,518 + Pf1N1B4_3266 0.39 +0.8 3,657,519 - Pf1N1B4_3266 0.39 -0.4 3,657,519 - Pf1N1B4_3266 0.39 +0.7 3,657,519 - Pf1N1B4_3266 0.39 -1.1 3,657,519 - Pf1N1B4_3266 0.39 +0.8 3,657,519 - Pf1N1B4_3266 0.39 -0.2 3,657,519 - Pf1N1B4_3266 0.39 +1.5 3,657,572 - Pf1N1B4_3266 0.48 +0.3 3,657,572 - Pf1N1B4_3266 0.48 -0.2 3,657,871 + -1.3 3,657,900 + +0.5 3,657,900 + +0.2 3,657,901 - -0.0 3,657,924 + Pf1N1B4_3267 0.12 +0.2 3,657,924 + Pf1N1B4_3267 0.12 -0.1 3,657,925 - Pf1N1B4_3267 0.12 +0.9 3,658,059 + Pf1N1B4_3267 0.56 +2.5 3,658,059 + Pf1N1B4_3267 0.56 +0.1 3,658,060 - Pf1N1B4_3267 0.56 +0.6 3,658,124 + Pf1N1B4_3267 0.77 +1.7 3,658,124 + Pf1N1B4_3267 0.77 -1.1 3,658,125 - Pf1N1B4_3267 0.77 +1.5 3,658,125 - Pf1N1B4_3267 0.77 -0.0 3,658,131 + Pf1N1B4_3267 0.79 +0.5 3,658,132 - Pf1N1B4_3267 0.80 +1.6 3,658,191 + -0.1 3,658,191 + +3.3 3,658,192 - -1.0 3,658,236 + +1.4 3,658,236 + +1.8 3,658,290 - -1.4 3,658,351 - -2.0 3,658,386 + -2.8 3,658,386 + +0.3 3,658,386 + +0.1 3,658,386 + -0.1 3,658,386 + -0.4 3,658,387 - -0.7 3,658,387 - -1.2 3,658,387 - +2.0 3,658,387 - -1.1 3,658,406 - +0.1 3,658,406 - -2.8 3,658,445 + -1.6 3,658,446 - Pf1N1B4_3268 0.15 +0.8 3,658,446 - Pf1N1B4_3268 0.15 -0.7 3,658,489 + Pf1N1B4_3268 0.28 -1.5 3,658,489 + Pf1N1B4_3268 0.28 -0.1 3,658,489 + Pf1N1B4_3268 0.28 -0.1 3,658,489 + Pf1N1B4_3268 0.28 +1.0 3,658,489 + Pf1N1B4_3268 0.28 -1.2 3,658,489 + Pf1N1B4_3268 0.28 -1.3 3,658,489 + Pf1N1B4_3268 0.28 -1.4 3,658,489 + Pf1N1B4_3268 0.28 -1.5 3,658,490 - Pf1N1B4_3268 0.28 -0.2 3,658,490 - Pf1N1B4_3268 0.28 +0.8 3,658,490 - Pf1N1B4_3268 0.28 +0.2 3,658,490 - Pf1N1B4_3268 0.28 -0.5 3,658,490 - Pf1N1B4_3268 0.28 -0.2 3,658,490 - Pf1N1B4_3268 0.28 -0.0 3,658,490 - Pf1N1B4_3268 0.28 -0.9 3,658,558 + Pf1N1B4_3268 0.48 +0.4 3,658,591 + Pf1N1B4_3268 0.57 -0.5 3,658,591 + Pf1N1B4_3268 0.57 -1.2 3,658,591 + Pf1N1B4_3268 0.57 +0.4 3,658,591 + Pf1N1B4_3268 0.57 -1.7 3,658,592 - Pf1N1B4_3268 0.58 +0.3 3,658,592 - Pf1N1B4_3268 0.58 +0.4 3,658,592 - Pf1N1B4_3268 0.58 -1.2 3,658,592 - Pf1N1B4_3268 0.58 -0.4 3,658,592 - Pf1N1B4_3268 0.58 -1.0 3,658,592 - Pf1N1B4_3268 0.58 +0.3 3,658,642 + Pf1N1B4_3268 0.72 +0.3 3,658,643 - Pf1N1B4_3268 0.72 +1.5 3,658,643 - Pf1N1B4_3268 0.72 -0.5 3,658,643 - Pf1N1B4_3268 0.72 -2.2 3,658,665 + Pf1N1B4_3268 0.79 -0.5 3,658,735 + +0.5 3,658,735 + -0.6 3,658,751 + -2.0 3,658,752 - +0.1 3,658,752 - +0.4 3,658,752 - +0.4 3,658,760 + -0.8 3,658,760 + +0.3 3,658,760 + -1.4 3,658,760 + -1.0 3,658,785 + +0.7 3,658,785 + -1.5 3,658,861 + +1.2 3,658,862 - -1.1 3,658,862 - +0.4 3,658,862 - +0.1 3,658,862 - -0.1 3,658,862 - -0.1 3,658,862 - +0.3 3,658,862 - -1.0 3,659,030 + -0.3 3,659,031 - +0.7 3,659,185 + Pf1N1B4_3269 0.25 -2.5
Or see this region's nucleotide sequence