Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3157 and Pf1N1B4_3158 are separated by 75 nucleotides Pf1N1B4_3158 and Pf1N1B4_3159 are separated by 18 nucleotides Pf1N1B4_3159 and Pf1N1B4_3160 are separated by 104 nucleotides
Pf1N1B4_3157 - FIG00956655: hypothetical protein, at 3,535,560 to 3,536,027
_3157
Pf1N1B4_3158 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 3,536,103 to 3,536,987
_3158
Pf1N1B4_3159 - Histone acetyltransferase HPA2 and related acetyltransferases, at 3,537,006 to 3,537,479
_3159
Pf1N1B4_3160 - Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase, at 3,537,584 to 3,538,921
_3160
Position (kb)
3537
3538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3536.010 kb on + strand at 3536.011 kb on - strand at 3536.011 kb on - strand at 3536.044 kb on - strand at 3536.064 kb on + strand at 3536.065 kb on - strand at 3536.085 kb on - strand at 3536.085 kb on - strand at 3536.085 kb on - strand at 3536.085 kb on - strand at 3536.085 kb on - strand at 3536.085 kb on - strand at 3536.085 kb on - strand at 3536.104 kb on + strand at 3536.104 kb on + strand at 3536.105 kb on - strand at 3536.105 kb on - strand at 3536.105 kb on - strand at 3536.105 kb on - strand at 3536.320 kb on + strand, within Pf1N1B4_3158 at 3536.321 kb on - strand, within Pf1N1B4_3158 at 3536.321 kb on - strand, within Pf1N1B4_3158 at 3536.321 kb on - strand, within Pf1N1B4_3158 at 3536.321 kb on - strand, within Pf1N1B4_3158 at 3536.321 kb on - strand, within Pf1N1B4_3158 at 3536.321 kb on - strand, within Pf1N1B4_3158 at 3536.386 kb on + strand, within Pf1N1B4_3158 at 3536.386 kb on + strand, within Pf1N1B4_3158 at 3536.386 kb on + strand, within Pf1N1B4_3158 at 3536.386 kb on + strand, within Pf1N1B4_3158 at 3536.386 kb on + strand, within Pf1N1B4_3158 at 3536.386 kb on + strand, within Pf1N1B4_3158 at 3536.386 kb on + strand, within Pf1N1B4_3158 at 3536.387 kb on - strand, within Pf1N1B4_3158 at 3536.387 kb on - strand, within Pf1N1B4_3158 at 3536.387 kb on - strand, within Pf1N1B4_3158 at 3536.387 kb on - strand, within Pf1N1B4_3158 at 3536.387 kb on - strand, within Pf1N1B4_3158 at 3536.847 kb on + strand, within Pf1N1B4_3158 at 3536.847 kb on + strand, within Pf1N1B4_3158 at 3536.847 kb on + strand at 3536.847 kb on + strand, within Pf1N1B4_3158 at 3536.848 kb on - strand, within Pf1N1B4_3158 at 3536.848 kb on - strand, within Pf1N1B4_3158 at 3536.929 kb on - strand at 3536.942 kb on + strand at 3536.943 kb on - strand at 3536.943 kb on - strand at 3536.943 kb on - strand at 3536.943 kb on - strand at 3536.943 kb on - strand at 3536.964 kb on - strand at 3536.964 kb on - strand at 3537.040 kb on - strand at 3537.062 kb on - strand, within Pf1N1B4_3159 at 3537.121 kb on + strand, within Pf1N1B4_3159 at 3537.121 kb on + strand, within Pf1N1B4_3159 at 3537.121 kb on + strand, within Pf1N1B4_3159 at 3537.121 kb on + strand, within Pf1N1B4_3159 at 3537.122 kb on - strand, within Pf1N1B4_3159 at 3537.122 kb on - strand, within Pf1N1B4_3159 at 3537.122 kb on - strand, within Pf1N1B4_3159 at 3537.122 kb on - strand, within Pf1N1B4_3159 at 3537.309 kb on - strand, within Pf1N1B4_3159 at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.370 kb on + strand at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.370 kb on + strand, within Pf1N1B4_3159 at 3537.371 kb on - strand at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.371 kb on - strand, within Pf1N1B4_3159 at 3537.436 kb on + strand at 3537.437 kb on - strand at 3537.526 kb on - strand at 3537.531 kb on + strand at 3537.531 kb on + strand at 3537.531 kb on + strand at 3537.532 kb on - strand at 3537.532 kb on - strand at 3537.572 kb on + strand at 3537.585 kb on + strand at 3537.586 kb on - strand at 3537.586 kb on - strand at 3537.586 kb on - strand at 3537.610 kb on - strand at 3537.961 kb on - strand, within Pf1N1B4_3160 at 3537.961 kb on - strand, within Pf1N1B4_3160 at 3538.138 kb on - strand, within Pf1N1B4_3160 at 3538.204 kb on - strand, within Pf1N1B4_3160 at 3538.204 kb on - strand at 3538.204 kb on - strand, within Pf1N1B4_3160 at 3538.318 kb on - strand, within Pf1N1B4_3160
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 3,536,010 + -0.1 3,536,011 - -1.7 3,536,011 - +0.4 3,536,044 - -1.7 3,536,064 + +0.3 3,536,065 - -0.3 3,536,085 - -1.0 3,536,085 - -0.8 3,536,085 - +0.2 3,536,085 - -0.8 3,536,085 - -0.1 3,536,085 - -1.0 3,536,085 - -0.2 3,536,104 + -0.3 3,536,104 + -0.5 3,536,105 - +1.5 3,536,105 - +0.4 3,536,105 - +0.3 3,536,105 - +0.0 3,536,320 + Pf1N1B4_3158 0.25 -0.7 3,536,321 - Pf1N1B4_3158 0.25 -1.0 3,536,321 - Pf1N1B4_3158 0.25 -0.9 3,536,321 - Pf1N1B4_3158 0.25 +0.4 3,536,321 - Pf1N1B4_3158 0.25 +1.7 3,536,321 - Pf1N1B4_3158 0.25 -0.4 3,536,321 - Pf1N1B4_3158 0.25 -3.2 3,536,386 + Pf1N1B4_3158 0.32 +1.0 3,536,386 + Pf1N1B4_3158 0.32 -0.4 3,536,386 + Pf1N1B4_3158 0.32 -0.0 3,536,386 + Pf1N1B4_3158 0.32 -1.3 3,536,386 + Pf1N1B4_3158 0.32 +1.4 3,536,386 + Pf1N1B4_3158 0.32 -0.1 3,536,386 + Pf1N1B4_3158 0.32 +0.4 3,536,387 - Pf1N1B4_3158 0.32 -1.2 3,536,387 - Pf1N1B4_3158 0.32 +0.4 3,536,387 - Pf1N1B4_3158 0.32 +1.6 3,536,387 - Pf1N1B4_3158 0.32 -0.6 3,536,387 - Pf1N1B4_3158 0.32 +0.0 3,536,847 + Pf1N1B4_3158 0.84 -0.1 3,536,847 + Pf1N1B4_3158 0.84 -0.4 3,536,847 + +2.0 3,536,847 + Pf1N1B4_3158 0.84 -1.3 3,536,848 - Pf1N1B4_3158 0.84 -1.2 3,536,848 - Pf1N1B4_3158 0.84 -0.2 3,536,929 - -1.4 3,536,942 + +0.1 3,536,943 - +0.1 3,536,943 - -1.6 3,536,943 - -1.0 3,536,943 - -0.4 3,536,943 - -2.0 3,536,964 - -1.0 3,536,964 - +1.3 3,537,040 - -1.7 3,537,062 - Pf1N1B4_3159 0.12 -0.9 3,537,121 + Pf1N1B4_3159 0.24 +0.5 3,537,121 + Pf1N1B4_3159 0.24 +0.6 3,537,121 + Pf1N1B4_3159 0.24 +2.0 3,537,121 + Pf1N1B4_3159 0.24 +1.5 3,537,122 - Pf1N1B4_3159 0.24 -1.0 3,537,122 - Pf1N1B4_3159 0.24 -1.8 3,537,122 - Pf1N1B4_3159 0.24 +0.7 3,537,122 - Pf1N1B4_3159 0.24 +0.3 3,537,309 - Pf1N1B4_3159 0.64 -0.2 3,537,370 + Pf1N1B4_3159 0.77 +1.7 3,537,370 + Pf1N1B4_3159 0.77 +0.8 3,537,370 + Pf1N1B4_3159 0.77 +1.5 3,537,370 + -0.4 3,537,370 + Pf1N1B4_3159 0.77 +0.7 3,537,370 + Pf1N1B4_3159 0.77 +0.3 3,537,371 - -1.8 3,537,371 - Pf1N1B4_3159 0.77 +1.5 3,537,371 - Pf1N1B4_3159 0.77 +0.6 3,537,371 - Pf1N1B4_3159 0.77 -1.0 3,537,371 - Pf1N1B4_3159 0.77 -0.8 3,537,371 - Pf1N1B4_3159 0.77 -1.5 3,537,371 - Pf1N1B4_3159 0.77 +0.5 3,537,371 - Pf1N1B4_3159 0.77 -1.3 3,537,371 - Pf1N1B4_3159 0.77 -0.0 3,537,371 - Pf1N1B4_3159 0.77 +0.8 3,537,436 + +0.0 3,537,437 - -0.1 3,537,526 - -1.4 3,537,531 + +1.6 3,537,531 + -0.1 3,537,531 + +0.3 3,537,532 - -0.3 3,537,532 - -0.3 3,537,572 + +0.7 3,537,585 + -1.0 3,537,586 - +0.5 3,537,586 - -0.7 3,537,586 - -0.2 3,537,610 - +0.5 3,537,961 - Pf1N1B4_3160 0.28 -1.0 3,537,961 - Pf1N1B4_3160 0.28 -0.3 3,538,138 - Pf1N1B4_3160 0.41 +0.3 3,538,204 - Pf1N1B4_3160 0.46 -0.3 3,538,204 - -1.5 3,538,204 - Pf1N1B4_3160 0.46 -1.9 3,538,318 - Pf1N1B4_3160 0.55 -0.3
Or see this region's nucleotide sequence