Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2410 and Pf1N1B4_2411 are separated by 223 nucleotides Pf1N1B4_2411 and Pf1N1B4_2412 are separated by 131 nucleotides
Pf1N1B4_2410 - FIG00956403: hypothetical protein, at 2,728,623 to 2,730,806
_2410
Pf1N1B4_2411 - FIG00953568: hypothetical protein, at 2,731,030 to 2,732,208
_2411
Pf1N1B4_2412 - ATP-dependent 23S rRNA helicase DbpA, at 2,732,340 to 2,733,725
_2412
Position (kb)
2731
2732
2733 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2730.061 kb on + strand, within Pf1N1B4_2410 at 2730.061 kb on + strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.422 kb on - strand, within Pf1N1B4_2410 at 2730.422 kb on - strand, within Pf1N1B4_2410 at 2730.522 kb on - strand, within Pf1N1B4_2410 at 2730.522 kb on - strand, within Pf1N1B4_2410 at 2730.838 kb on + strand at 2730.839 kb on - strand at 2730.851 kb on + strand at 2730.851 kb on + strand at 2730.917 kb on - strand at 2730.917 kb on - strand at 2730.958 kb on - strand at 2730.958 kb on - strand at 2731.017 kb on + strand at 2731.017 kb on + strand at 2731.017 kb on + strand at 2731.017 kb on + strand at 2731.018 kb on - strand at 2731.127 kb on + strand at 2731.128 kb on - strand at 2731.168 kb on + strand, within Pf1N1B4_2411 at 2731.169 kb on - strand, within Pf1N1B4_2411 at 2731.289 kb on + strand, within Pf1N1B4_2411 at 2731.290 kb on - strand, within Pf1N1B4_2411 at 2731.290 kb on - strand at 2731.980 kb on - strand, within Pf1N1B4_2411 at 2732.379 kb on - strand at 2732.434 kb on + strand at 2732.435 kb on - strand at 2732.435 kb on - strand at 2732.435 kb on - strand at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.792 kb on + strand, within Pf1N1B4_2412 at 2732.843 kb on + strand at 2732.844 kb on - strand, within Pf1N1B4_2412 at 2732.844 kb on - strand, within Pf1N1B4_2412 at 2732.869 kb on - strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.056 kb on - strand, within Pf1N1B4_2412
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 2,730,061 + Pf1N1B4_2410 0.66 -0.8 2,730,061 + Pf1N1B4_2410 0.66 +0.0 2,730,245 - Pf1N1B4_2410 0.74 -3.0 2,730,245 - Pf1N1B4_2410 0.74 +0.8 2,730,245 - Pf1N1B4_2410 0.74 -1.2 2,730,245 - Pf1N1B4_2410 0.74 -1.5 2,730,245 - Pf1N1B4_2410 0.74 -0.8 2,730,245 - Pf1N1B4_2410 0.74 +0.3 2,730,422 - Pf1N1B4_2410 0.82 -1.3 2,730,422 - Pf1N1B4_2410 0.82 -1.1 2,730,522 - Pf1N1B4_2410 0.87 -1.0 2,730,522 - Pf1N1B4_2410 0.87 -1.5 2,730,838 + +0.4 2,730,839 - +1.1 2,730,851 + +0.2 2,730,851 + +0.3 2,730,917 - -0.5 2,730,917 - -2.1 2,730,958 - +0.3 2,730,958 - +2.4 2,731,017 + +0.3 2,731,017 + -0.2 2,731,017 + -0.0 2,731,017 + +1.3 2,731,018 - +1.9 2,731,127 + -1.4 2,731,128 - +1.2 2,731,168 + Pf1N1B4_2411 0.12 -0.7 2,731,169 - Pf1N1B4_2411 0.12 -0.2 2,731,289 + Pf1N1B4_2411 0.22 -0.5 2,731,290 - Pf1N1B4_2411 0.22 -1.7 2,731,290 - +1.7 2,731,980 - Pf1N1B4_2411 0.81 -1.0 2,732,379 - +0.1 2,732,434 + +0.1 2,732,435 - +0.5 2,732,435 - -1.3 2,732,435 - +0.2 2,732,527 - Pf1N1B4_2412 0.13 -1.3 2,732,527 - Pf1N1B4_2412 0.13 -0.3 2,732,527 - Pf1N1B4_2412 0.13 -0.7 2,732,527 - Pf1N1B4_2412 0.13 +0.1 2,732,792 + Pf1N1B4_2412 0.33 +1.1 2,732,843 + -0.7 2,732,844 - Pf1N1B4_2412 0.36 -1.5 2,732,844 - Pf1N1B4_2412 0.36 +0.5 2,732,869 - Pf1N1B4_2412 0.38 -0.1 2,733,055 + Pf1N1B4_2412 0.52 +2.0 2,733,055 + Pf1N1B4_2412 0.52 +0.5 2,733,055 + Pf1N1B4_2412 0.52 -2.3 2,733,056 - Pf1N1B4_2412 0.52 -0.7
Or see this region's nucleotide sequence