Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_233

Experiment: nitrogen source Ammonium chloride 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_231 and Pf1N1B4_232 are separated by 47 nucleotidesPf1N1B4_232 and Pf1N1B4_233 overlap by 17 nucleotidesPf1N1B4_233 and Pf1N1B4_234 are separated by 307 nucleotidesPf1N1B4_234 and Pf1N1B4_235 are separated by 65 nucleotides Pf1N1B4_231 - Sensor histidine kinase, at 292,071 to 292,964 _231 Pf1N1B4_232 - DNA-binding response regulator, LuxR family, at 293,012 to 293,620 _232 Pf1N1B4_233 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 293,604 to 294,536 _233 Pf1N1B4_234 - hypothetical protein, at 294,844 to 295,152 _234 Pf1N1B4_235 - Molybdopterin biosynthesis protein MoeA, at 295,218 to 296,405 _235 Position (kb) 293 294 295Strain fitness (log2 ratio) -2 -1 0 1 2at 292.682 kb on + strand, within Pf1N1B4_231at 292.682 kb on + strand, within Pf1N1B4_231at 292.683 kb on - strandat 292.684 kb on + strand, within Pf1N1B4_231at 292.684 kb on + strand, within Pf1N1B4_231at 292.684 kb on + strand, within Pf1N1B4_231at 292.706 kb on + strand, within Pf1N1B4_231at 292.849 kb on + strand, within Pf1N1B4_231at 292.849 kb on + strand, within Pf1N1B4_231at 292.931 kb on - strandat 292.949 kb on + strandat 292.950 kb on - strandat 292.969 kb on + strandat 292.969 kb on + strandat 292.970 kb on - strandat 292.974 kb on + strandat 292.974 kb on + strandat 292.974 kb on + strandat 292.974 kb on + strandat 292.975 kb on - strandat 292.975 kb on - strandat 292.999 kb on + strandat 293.049 kb on + strandat 293.125 kb on + strandat 293.558 kb on + strand, within Pf1N1B4_232at 293.559 kb on - strand, within Pf1N1B4_232at 293.559 kb on - strand, within Pf1N1B4_232at 293.735 kb on + strand, within Pf1N1B4_233at 293.736 kb on - strand, within Pf1N1B4_233at 293.736 kb on - strandat 293.737 kb on + strandat 293.738 kb on - strandat 293.738 kb on - strand, within Pf1N1B4_233at 293.738 kb on - strand, within Pf1N1B4_233at 293.738 kb on - strand, within Pf1N1B4_233at 293.738 kb on - strand, within Pf1N1B4_233at 293.738 kb on - strandat 293.738 kb on - strand, within Pf1N1B4_233at 294.046 kb on + strand, within Pf1N1B4_233at 294.046 kb on + strand, within Pf1N1B4_233at 294.047 kb on - strand, within Pf1N1B4_233at 294.087 kb on - strand, within Pf1N1B4_233at 294.087 kb on - strand, within Pf1N1B4_233at 294.087 kb on - strand, within Pf1N1B4_233at 294.264 kb on + strand, within Pf1N1B4_233at 294.265 kb on - strandat 294.351 kb on - strand, within Pf1N1B4_233at 294.555 kb on + strandat 294.555 kb on + strandat 294.555 kb on + strandat 294.556 kb on - strandat 294.556 kb on - strandat 294.578 kb on + strandat 294.579 kb on - strandat 294.579 kb on - strandat 294.600 kb on + strandat 294.601 kb on - strandat 294.628 kb on + strandat 294.649 kb on - strandat 294.649 kb on - strandat 294.694 kb on + strandat 294.695 kb on - strandat 294.695 kb on - strandat 294.730 kb on + strandat 294.734 kb on + strandat 294.735 kb on - strandat 294.735 kb on - strandat 294.821 kb on + strandat 294.997 kb on + strandat 294.997 kb on + strand, within Pf1N1B4_234at 294.997 kb on + strand, within Pf1N1B4_234at 294.997 kb on + strand, within Pf1N1B4_234at 294.998 kb on - strand, within Pf1N1B4_234at 294.998 kb on - strand, within Pf1N1B4_234at 295.150 kb on + strandat 295.150 kb on + strandat 295.150 kb on + strandat 295.216 kb on + strandat 295.217 kb on - strandat 295.275 kb on - strandat 295.348 kb on + strand, within Pf1N1B4_235at 295.349 kb on - strand, within Pf1N1B4_235at 295.387 kb on + strand, within Pf1N1B4_235at 295.388 kb on - strand, within Pf1N1B4_235at 295.388 kb on - strand, within Pf1N1B4_235at 295.388 kb on - strand, within Pf1N1B4_235at 295.483 kb on + strand, within Pf1N1B4_235

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM
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292,682 + Pf1N1B4_231 0.68 -0.3
292,682 + Pf1N1B4_231 0.68 -2.1
292,683 - +0.5
292,684 + Pf1N1B4_231 0.69 -0.6
292,684 + Pf1N1B4_231 0.69 -0.7
292,684 + Pf1N1B4_231 0.69 -0.5
292,706 + Pf1N1B4_231 0.71 +0.2
292,849 + Pf1N1B4_231 0.87 +0.4
292,849 + Pf1N1B4_231 0.87 -1.2
292,931 - +1.0
292,949 + -0.3
292,950 - +0.2
292,969 + +0.8
292,969 + -0.3
292,970 - -0.6
292,974 + +0.7
292,974 + +0.0
292,974 + -0.7
292,974 + -0.8
292,975 - +0.2
292,975 - -0.6
292,999 + -1.8
293,049 + +0.7
293,125 + +2.2
293,558 + Pf1N1B4_232 0.90 +0.5
293,559 - Pf1N1B4_232 0.90 -1.3
293,559 - Pf1N1B4_232 0.90 +1.6
293,735 + Pf1N1B4_233 0.14 -0.5
293,736 - Pf1N1B4_233 0.14 +1.1
293,736 - -0.8
293,737 + -1.1
293,738 - -0.9
293,738 - Pf1N1B4_233 0.14 +0.5
293,738 - Pf1N1B4_233 0.14 +0.6
293,738 - Pf1N1B4_233 0.14 -1.5
293,738 - Pf1N1B4_233 0.14 +0.9
293,738 - +1.0
293,738 - Pf1N1B4_233 0.14 +0.6
294,046 + Pf1N1B4_233 0.47 +0.6
294,046 + Pf1N1B4_233 0.47 +1.2
294,047 - Pf1N1B4_233 0.47 -1.3
294,087 - Pf1N1B4_233 0.52 +0.8
294,087 - Pf1N1B4_233 0.52 -0.9
294,087 - Pf1N1B4_233 0.52 +1.1
294,264 + Pf1N1B4_233 0.71 +0.4
294,265 - -1.2
294,351 - Pf1N1B4_233 0.80 +1.0
294,555 + +0.2
294,555 + -0.0
294,555 + -0.4
294,556 - +1.3
294,556 - -0.1
294,578 + -1.3
294,579 - +0.8
294,579 - -0.2
294,600 + +1.1
294,601 - +1.7
294,628 + +0.3
294,649 - -0.6
294,649 - -0.1
294,694 + -1.6
294,695 - +0.8
294,695 - +0.1
294,730 + -0.5
294,734 + -1.4
294,735 - -1.5
294,735 - +0.1
294,821 + +0.7
294,997 + -0.5
294,997 + Pf1N1B4_234 0.50 -0.4
294,997 + Pf1N1B4_234 0.50 +0.2
294,997 + Pf1N1B4_234 0.50 +0.2
294,998 - Pf1N1B4_234 0.50 -0.4
294,998 - Pf1N1B4_234 0.50 -1.2
295,150 + -0.1
295,150 + -1.2
295,150 + -2.0
295,216 + -2.6
295,217 - +1.0
295,275 - -1.5
295,348 + Pf1N1B4_235 0.11 -0.8
295,349 - Pf1N1B4_235 0.11 +0.4
295,387 + Pf1N1B4_235 0.14 +1.3
295,388 - Pf1N1B4_235 0.14 -2.1
295,388 - Pf1N1B4_235 0.14 +0.9
295,388 - Pf1N1B4_235 0.14 -0.1
295,483 + Pf1N1B4_235 0.22 +0.1

Or see this region's nucleotide sequence