Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1706 and Pf1N1B4_1707 overlap by 4 nucleotides Pf1N1B4_1707 and Pf1N1B4_1708 overlap by 4 nucleotides
Pf1N1B4_1706 - RND efflux transporter, at 1,951,667 to 1,954,720
_1706
Pf1N1B4_1707 - Efflux membrane fusion protein, RND family, at 1,954,717 to 1,955,784
_1707
Pf1N1B4_1708 - Efflux transporter, RND family, MFP subunit, at 1,955,781 to 1,956,884
_1708
Position (kb)
1954
1955
1956 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1953.731 kb on + strand, within Pf1N1B4_1706 at 1953.731 kb on + strand, within Pf1N1B4_1706 at 1953.731 kb on + strand, within Pf1N1B4_1706 at 1953.731 kb on + strand, within Pf1N1B4_1706 at 1953.731 kb on + strand, within Pf1N1B4_1706 at 1953.732 kb on - strand, within Pf1N1B4_1706 at 1953.755 kb on - strand, within Pf1N1B4_1706 at 1953.915 kb on + strand, within Pf1N1B4_1706 at 1953.916 kb on - strand, within Pf1N1B4_1706 at 1953.916 kb on - strand, within Pf1N1B4_1706 at 1953.951 kb on + strand, within Pf1N1B4_1706 at 1954.152 kb on + strand, within Pf1N1B4_1706 at 1954.153 kb on - strand, within Pf1N1B4_1706 at 1954.153 kb on - strand, within Pf1N1B4_1706 at 1954.153 kb on - strand, within Pf1N1B4_1706 at 1954.223 kb on + strand, within Pf1N1B4_1706 at 1954.224 kb on - strand, within Pf1N1B4_1706 at 1954.224 kb on - strand, within Pf1N1B4_1706 at 1954.224 kb on - strand, within Pf1N1B4_1706 at 1954.224 kb on - strand, within Pf1N1B4_1706 at 1954.224 kb on - strand, within Pf1N1B4_1706 at 1954.236 kb on + strand, within Pf1N1B4_1706 at 1954.281 kb on + strand, within Pf1N1B4_1706 at 1954.281 kb on + strand at 1954.281 kb on + strand, within Pf1N1B4_1706 at 1954.282 kb on - strand, within Pf1N1B4_1706 at 1954.462 kb on - strand at 1954.474 kb on - strand at 1954.474 kb on - strand at 1954.623 kb on + strand at 1954.623 kb on + strand at 1954.624 kb on - strand at 1954.662 kb on + strand at 1954.662 kb on + strand at 1954.662 kb on + strand at 1954.663 kb on - strand at 1954.701 kb on - strand at 1955.237 kb on - strand at 1955.291 kb on - strand at 1955.291 kb on - strand, within Pf1N1B4_1707 at 1955.300 kb on + strand, within Pf1N1B4_1707 at 1955.300 kb on + strand, within Pf1N1B4_1707 at 1955.300 kb on + strand, within Pf1N1B4_1707 at 1955.301 kb on - strand, within Pf1N1B4_1707 at 1955.301 kb on - strand, within Pf1N1B4_1707 at 1955.301 kb on - strand, within Pf1N1B4_1707 at 1955.301 kb on - strand, within Pf1N1B4_1707 at 1955.812 kb on + strand at 1955.813 kb on - strand at 1955.813 kb on - strand at 1955.813 kb on - strand at 1955.813 kb on - strand at 1955.853 kb on + strand at 1955.853 kb on + strand at 1955.853 kb on + strand at 1955.854 kb on - strand at 1956.221 kb on + strand, within Pf1N1B4_1708 at 1956.254 kb on - strand, within Pf1N1B4_1708 at 1956.254 kb on - strand, within Pf1N1B4_1708 at 1956.318 kb on + strand, within Pf1N1B4_1708 at 1956.318 kb on + strand, within Pf1N1B4_1708 at 1956.319 kb on - strand, within Pf1N1B4_1708 at 1956.319 kb on - strand, within Pf1N1B4_1708 at 1956.319 kb on - strand, within Pf1N1B4_1708 at 1956.319 kb on - strand, within Pf1N1B4_1708 at 1956.385 kb on + strand, within Pf1N1B4_1708 at 1956.386 kb on - strand, within Pf1N1B4_1708 at 1956.475 kb on + strand, within Pf1N1B4_1708 at 1956.476 kb on - strand, within Pf1N1B4_1708 at 1956.493 kb on + strand, within Pf1N1B4_1708
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 1,953,731 + Pf1N1B4_1706 0.68 +1.5 1,953,731 + Pf1N1B4_1706 0.68 +1.1 1,953,731 + Pf1N1B4_1706 0.68 -0.7 1,953,731 + Pf1N1B4_1706 0.68 -0.2 1,953,731 + Pf1N1B4_1706 0.68 +0.9 1,953,732 - Pf1N1B4_1706 0.68 -1.4 1,953,755 - Pf1N1B4_1706 0.68 -0.5 1,953,915 + Pf1N1B4_1706 0.74 -0.7 1,953,916 - Pf1N1B4_1706 0.74 -1.2 1,953,916 - Pf1N1B4_1706 0.74 +0.2 1,953,951 + Pf1N1B4_1706 0.75 -0.3 1,954,152 + Pf1N1B4_1706 0.81 +1.4 1,954,153 - Pf1N1B4_1706 0.81 -0.9 1,954,153 - Pf1N1B4_1706 0.81 +0.3 1,954,153 - Pf1N1B4_1706 0.81 -1.6 1,954,223 + Pf1N1B4_1706 0.84 -0.5 1,954,224 - Pf1N1B4_1706 0.84 -1.2 1,954,224 - Pf1N1B4_1706 0.84 -2.2 1,954,224 - Pf1N1B4_1706 0.84 +0.7 1,954,224 - Pf1N1B4_1706 0.84 +1.0 1,954,224 - Pf1N1B4_1706 0.84 -1.8 1,954,236 + Pf1N1B4_1706 0.84 +1.0 1,954,281 + Pf1N1B4_1706 0.86 -0.4 1,954,281 + -1.4 1,954,281 + Pf1N1B4_1706 0.86 -0.4 1,954,282 - Pf1N1B4_1706 0.86 -2.2 1,954,462 - +0.0 1,954,474 - -0.6 1,954,474 - -1.1 1,954,623 + +0.8 1,954,623 + +0.4 1,954,624 - -1.2 1,954,662 + -0.5 1,954,662 + +0.6 1,954,662 + +2.4 1,954,663 - -1.5 1,954,701 - -0.1 1,955,237 - -0.6 1,955,291 - -0.2 1,955,291 - Pf1N1B4_1707 0.54 -1.7 1,955,300 + Pf1N1B4_1707 0.55 -1.4 1,955,300 + Pf1N1B4_1707 0.55 +0.2 1,955,300 + Pf1N1B4_1707 0.55 -1.2 1,955,301 - Pf1N1B4_1707 0.55 -0.9 1,955,301 - Pf1N1B4_1707 0.55 -0.4 1,955,301 - Pf1N1B4_1707 0.55 -1.2 1,955,301 - Pf1N1B4_1707 0.55 -0.5 1,955,812 + +1.0 1,955,813 - +0.4 1,955,813 - -1.1 1,955,813 - -2.3 1,955,813 - -1.0 1,955,853 + +0.4 1,955,853 + +0.6 1,955,853 + -0.0 1,955,854 - +0.7 1,956,221 + Pf1N1B4_1708 0.40 -0.4 1,956,254 - Pf1N1B4_1708 0.43 -1.7 1,956,254 - Pf1N1B4_1708 0.43 -2.7 1,956,318 + Pf1N1B4_1708 0.49 +0.5 1,956,318 + Pf1N1B4_1708 0.49 -1.0 1,956,319 - Pf1N1B4_1708 0.49 +0.1 1,956,319 - Pf1N1B4_1708 0.49 +1.9 1,956,319 - Pf1N1B4_1708 0.49 +0.2 1,956,319 - Pf1N1B4_1708 0.49 +3.3 1,956,385 + Pf1N1B4_1708 0.55 +0.5 1,956,386 - Pf1N1B4_1708 0.55 -0.2 1,956,475 + Pf1N1B4_1708 0.63 -0.9 1,956,476 - Pf1N1B4_1708 0.63 -0.0 1,956,493 + Pf1N1B4_1708 0.64 +2.4
Or see this region's nucleotide sequence