Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1055 and Pf1N1B4_1056 are separated by 0 nucleotides Pf1N1B4_1056 and Pf1N1B4_1057 are separated by 181 nucleotides
Pf1N1B4_1055 - Phosphate acetyltransferase (EC 2.3.1.8), at 1,189,837 to 1,191,105
_1055
Pf1N1B4_1056 - FIG018329: 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,191,106 to 1,192,014
_1056
Pf1N1B4_1057 - Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25), at 1,192,196 to 1,194,094
_1057
Position (kb)
1191
1192
1193 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1190.353 kb on + strand, within Pf1N1B4_1055 at 1190.385 kb on + strand, within Pf1N1B4_1055 at 1190.385 kb on + strand, within Pf1N1B4_1055 at 1190.385 kb on + strand, within Pf1N1B4_1055 at 1190.385 kb on + strand, within Pf1N1B4_1055 at 1190.386 kb on - strand, within Pf1N1B4_1055 at 1190.386 kb on - strand, within Pf1N1B4_1055 at 1190.605 kb on + strand, within Pf1N1B4_1055 at 1190.722 kb on + strand, within Pf1N1B4_1055 at 1190.722 kb on + strand, within Pf1N1B4_1055 at 1190.722 kb on + strand at 1190.722 kb on + strand, within Pf1N1B4_1055 at 1190.831 kb on - strand, within Pf1N1B4_1055 at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.052 kb on + strand at 1191.053 kb on - strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.093 kb on + strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.094 kb on - strand at 1191.103 kb on + strand at 1191.103 kb on + strand at 1191.119 kb on + strand at 1191.359 kb on - strand, within Pf1N1B4_1056 at 1191.370 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.422 kb on - strand, within Pf1N1B4_1056 at 1191.422 kb on - strand, within Pf1N1B4_1056 at 1191.548 kb on - strand, within Pf1N1B4_1056 at 1191.680 kb on + strand, within Pf1N1B4_1056 at 1191.680 kb on + strand, within Pf1N1B4_1056 at 1191.680 kb on + strand, within Pf1N1B4_1056 at 1191.681 kb on - strand, within Pf1N1B4_1056 at 1191.681 kb on - strand, within Pf1N1B4_1056 at 1191.901 kb on + strand, within Pf1N1B4_1056 at 1191.902 kb on - strand, within Pf1N1B4_1056 at 1191.997 kb on + strand at 1191.997 kb on + strand at 1191.997 kb on + strand at 1191.998 kb on - strand at 1192.180 kb on + strand at 1192.198 kb on - strand at 1192.213 kb on + strand at 1192.345 kb on - strand at 1192.395 kb on + strand, within Pf1N1B4_1057 at 1192.396 kb on - strand, within Pf1N1B4_1057 at 1192.751 kb on + strand, within Pf1N1B4_1057 at 1192.752 kb on - strand, within Pf1N1B4_1057 at 1192.752 kb on - strand, within Pf1N1B4_1057
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 1,190,353 + Pf1N1B4_1055 0.41 +2.3 1,190,385 + Pf1N1B4_1055 0.43 +1.1 1,190,385 + Pf1N1B4_1055 0.43 -0.0 1,190,385 + Pf1N1B4_1055 0.43 -0.1 1,190,385 + Pf1N1B4_1055 0.43 -0.3 1,190,386 - Pf1N1B4_1055 0.43 +1.5 1,190,386 - Pf1N1B4_1055 0.43 +1.2 1,190,605 + Pf1N1B4_1055 0.61 -1.4 1,190,722 + Pf1N1B4_1055 0.70 -0.5 1,190,722 + Pf1N1B4_1055 0.70 +1.4 1,190,722 + -0.1 1,190,722 + Pf1N1B4_1055 0.70 +1.4 1,190,831 - Pf1N1B4_1055 0.78 +0.2 1,191,052 + +0.9 1,191,052 + +1.6 1,191,052 + -0.1 1,191,052 + -0.8 1,191,052 + -0.3 1,191,052 + -0.4 1,191,053 - +1.6 1,191,093 + -0.2 1,191,093 + +0.6 1,191,093 + -0.3 1,191,093 + -0.1 1,191,093 + +0.1 1,191,093 + -0.4 1,191,093 + +0.4 1,191,093 + -0.0 1,191,094 - -0.4 1,191,094 - -0.4 1,191,094 - -0.5 1,191,094 - -0.4 1,191,094 - +2.3 1,191,094 - +0.2 1,191,103 + -1.5 1,191,103 + +1.6 1,191,119 + +0.7 1,191,359 - Pf1N1B4_1056 0.28 -0.9 1,191,370 + Pf1N1B4_1056 0.29 -0.3 1,191,421 + Pf1N1B4_1056 0.35 -2.5 1,191,421 + Pf1N1B4_1056 0.35 +0.1 1,191,421 + Pf1N1B4_1056 0.35 -0.6 1,191,421 + Pf1N1B4_1056 0.35 -0.7 1,191,422 - Pf1N1B4_1056 0.35 -0.5 1,191,422 - Pf1N1B4_1056 0.35 -1.4 1,191,548 - Pf1N1B4_1056 0.49 -2.1 1,191,680 + Pf1N1B4_1056 0.63 -0.9 1,191,680 + Pf1N1B4_1056 0.63 +0.1 1,191,680 + Pf1N1B4_1056 0.63 -2.7 1,191,681 - Pf1N1B4_1056 0.63 -0.8 1,191,681 - Pf1N1B4_1056 0.63 -0.9 1,191,901 + Pf1N1B4_1056 0.87 +0.9 1,191,902 - Pf1N1B4_1056 0.88 -0.4 1,191,997 + +0.2 1,191,997 + -2.0 1,191,997 + -1.7 1,191,998 - +0.1 1,192,180 + +0.7 1,192,198 - +0.8 1,192,213 + +0.3 1,192,345 - -2.3 1,192,395 + Pf1N1B4_1057 0.10 -0.1 1,192,396 - Pf1N1B4_1057 0.11 -0.9 1,192,751 + Pf1N1B4_1057 0.29 -1.7 1,192,752 - Pf1N1B4_1057 0.29 -1.9 1,192,752 - Pf1N1B4_1057 0.29 -0.9
Or see this region's nucleotide sequence