Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_83 and Pf1N1B4_84 are separated by 250 nucleotides Pf1N1B4_84 and Pf1N1B4_85 are separated by 130 nucleotides Pf1N1B4_85 and Pf1N1B4_86 are separated by 29 nucleotides
Pf1N1B4_83 - putative lipoprotein, at 86,235 to 86,984
_83
Pf1N1B4_84 - Aldose 1-epimerase (EC 5.1.3.3), at 87,235 to 88,383
_84
Pf1N1B4_85 - Oxidoreductase, aldo/keto reductase family, at 88,514 to 89,335
_85
Pf1N1B4_86 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 89,365 to 89,835
_86
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 86.236 kb on + strand at 86.237 kb on - strand at 86.237 kb on - strand at 86.237 kb on - strand at 86.278 kb on + strand at 86.278 kb on + strand at 86.278 kb on + strand at 86.278 kb on + strand at 86.279 kb on - strand at 86.515 kb on + strand, within Pf1N1B4_83 at 86.515 kb on + strand, within Pf1N1B4_83 at 86.515 kb on + strand, within Pf1N1B4_83 at 86.515 kb on + strand at 86.516 kb on - strand, within Pf1N1B4_83 at 86.516 kb on - strand, within Pf1N1B4_83 at 86.516 kb on - strand, within Pf1N1B4_83 at 86.566 kb on + strand, within Pf1N1B4_83 at 86.566 kb on + strand, within Pf1N1B4_83 at 86.566 kb on + strand, within Pf1N1B4_83 at 86.567 kb on - strand, within Pf1N1B4_83 at 86.567 kb on - strand, within Pf1N1B4_83 at 86.567 kb on - strand, within Pf1N1B4_83 at 86.567 kb on - strand, within Pf1N1B4_83 at 86.567 kb on - strand, within Pf1N1B4_83 at 86.567 kb on - strand, within Pf1N1B4_83 at 86.570 kb on + strand, within Pf1N1B4_83 at 86.570 kb on + strand, within Pf1N1B4_83 at 86.570 kb on + strand, within Pf1N1B4_83 at 86.571 kb on - strand, within Pf1N1B4_83 at 86.571 kb on - strand, within Pf1N1B4_83 at 86.571 kb on - strand, within Pf1N1B4_83 at 86.571 kb on - strand, within Pf1N1B4_83 at 86.571 kb on - strand, within Pf1N1B4_83 at 86.614 kb on + strand, within Pf1N1B4_83 at 86.614 kb on + strand, within Pf1N1B4_83 at 86.614 kb on + strand, within Pf1N1B4_83 at 86.615 kb on - strand, within Pf1N1B4_83 at 86.615 kb on - strand, within Pf1N1B4_83 at 86.706 kb on + strand, within Pf1N1B4_83 at 86.706 kb on + strand, within Pf1N1B4_83 at 86.706 kb on + strand, within Pf1N1B4_83 at 86.707 kb on - strand, within Pf1N1B4_83 at 86.707 kb on - strand, within Pf1N1B4_83 at 86.707 kb on - strand, within Pf1N1B4_83 at 86.753 kb on - strand, within Pf1N1B4_83 at 86.774 kb on - strand, within Pf1N1B4_83 at 87.042 kb on + strand at 87.042 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.149 kb on + strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.150 kb on - strand at 87.370 kb on + strand, within Pf1N1B4_84 at 87.370 kb on + strand, within Pf1N1B4_84 at 87.370 kb on + strand, within Pf1N1B4_84 at 87.370 kb on + strand, within Pf1N1B4_84 at 87.370 kb on + strand, within Pf1N1B4_84 at 87.370 kb on + strand, within Pf1N1B4_84 at 87.371 kb on - strand at 87.415 kb on + strand, within Pf1N1B4_84 at 87.473 kb on + strand, within Pf1N1B4_84 at 87.474 kb on - strand at 87.474 kb on - strand, within Pf1N1B4_84 at 87.474 kb on - strand, within Pf1N1B4_84 at 87.474 kb on - strand, within Pf1N1B4_84 at 87.651 kb on + strand at 87.712 kb on + strand, within Pf1N1B4_84 at 87.712 kb on + strand, within Pf1N1B4_84 at 87.713 kb on - strand, within Pf1N1B4_84 at 87.713 kb on - strand, within Pf1N1B4_84 at 87.713 kb on - strand, within Pf1N1B4_84 at 87.713 kb on - strand, within Pf1N1B4_84 at 87.769 kb on + strand, within Pf1N1B4_84 at 87.770 kb on - strand, within Pf1N1B4_84 at 87.806 kb on - strand, within Pf1N1B4_84 at 87.806 kb on - strand, within Pf1N1B4_84 at 87.806 kb on - strand, within Pf1N1B4_84 at 87.856 kb on + strand, within Pf1N1B4_84 at 87.856 kb on + strand, within Pf1N1B4_84 at 87.890 kb on + strand, within Pf1N1B4_84 at 87.890 kb on + strand, within Pf1N1B4_84 at 87.891 kb on - strand, within Pf1N1B4_84 at 87.925 kb on + strand, within Pf1N1B4_84 at 88.168 kb on + strand, within Pf1N1B4_84 at 88.168 kb on + strand, within Pf1N1B4_84 at 88.169 kb on - strand, within Pf1N1B4_84 at 88.169 kb on - strand, within Pf1N1B4_84 at 88.173 kb on + strand, within Pf1N1B4_84 at 88.195 kb on + strand at 88.195 kb on + strand, within Pf1N1B4_84 at 88.195 kb on + strand, within Pf1N1B4_84 at 88.195 kb on + strand at 88.196 kb on - strand, within Pf1N1B4_84 at 88.196 kb on - strand, within Pf1N1B4_84 at 88.196 kb on - strand, within Pf1N1B4_84 at 88.197 kb on + strand, within Pf1N1B4_84 at 88.198 kb on - strand, within Pf1N1B4_84 at 88.345 kb on + strand at 88.345 kb on + strand at 88.345 kb on + strand at 88.346 kb on - strand at 88.346 kb on - strand at 88.346 kb on - strand at 88.347 kb on + strand at 88.363 kb on + strand at 88.363 kb on + strand at 88.363 kb on + strand at 88.363 kb on + strand at 88.364 kb on - strand at 88.381 kb on + strand at 88.382 kb on - strand at 88.422 kb on - strand at 88.485 kb on + strand at 88.500 kb on - strand at 88.519 kb on + strand at 88.520 kb on - strand at 88.617 kb on + strand, within Pf1N1B4_85 at 88.617 kb on + strand, within Pf1N1B4_85 at 88.667 kb on + strand, within Pf1N1B4_85 at 88.668 kb on - strand, within Pf1N1B4_85 at 88.754 kb on + strand, within Pf1N1B4_85 at 88.754 kb on + strand, within Pf1N1B4_85 at 88.755 kb on - strand, within Pf1N1B4_85 at 88.820 kb on + strand, within Pf1N1B4_85 at 88.820 kb on + strand, within Pf1N1B4_85 at 88.994 kb on + strand, within Pf1N1B4_85 at 88.994 kb on + strand, within Pf1N1B4_85 at 88.995 kb on - strand, within Pf1N1B4_85 at 88.996 kb on + strand at 89.070 kb on - strand, within Pf1N1B4_85 at 89.070 kb on - strand, within Pf1N1B4_85 at 89.070 kb on - strand, within Pf1N1B4_85 at 89.311 kb on + strand at 89.311 kb on + strand at 89.312 kb on - strand at 89.312 kb on - strand at 89.312 kb on - strand at 89.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 86,236 + -3.5 86,237 - -2.2 86,237 - +0.3 86,237 - -0.5 86,278 + +0.1 86,278 + -1.5 86,278 + -0.4 86,278 + -1.1 86,279 - -1.4 86,515 + Pf1N1B4_83 0.37 -1.5 86,515 + Pf1N1B4_83 0.37 -0.8 86,515 + Pf1N1B4_83 0.37 +0.5 86,515 + -0.9 86,516 - Pf1N1B4_83 0.37 -1.1 86,516 - Pf1N1B4_83 0.37 -0.9 86,516 - Pf1N1B4_83 0.37 +2.0 86,566 + Pf1N1B4_83 0.44 -1.0 86,566 + Pf1N1B4_83 0.44 -2.1 86,566 + Pf1N1B4_83 0.44 -0.7 86,567 - Pf1N1B4_83 0.44 -2.4 86,567 - Pf1N1B4_83 0.44 -2.9 86,567 - Pf1N1B4_83 0.44 +0.5 86,567 - Pf1N1B4_83 0.44 +0.7 86,567 - Pf1N1B4_83 0.44 -1.5 86,567 - Pf1N1B4_83 0.44 +1.1 86,570 + Pf1N1B4_83 0.45 -1.6 86,570 + Pf1N1B4_83 0.45 -0.5 86,570 + Pf1N1B4_83 0.45 -0.6 86,571 - Pf1N1B4_83 0.45 -1.2 86,571 - Pf1N1B4_83 0.45 +1.5 86,571 - Pf1N1B4_83 0.45 -0.1 86,571 - Pf1N1B4_83 0.45 -1.7 86,571 - Pf1N1B4_83 0.45 -0.1 86,614 + Pf1N1B4_83 0.51 -0.4 86,614 + Pf1N1B4_83 0.51 -1.9 86,614 + Pf1N1B4_83 0.51 -1.3 86,615 - Pf1N1B4_83 0.51 -0.2 86,615 - Pf1N1B4_83 0.51 -0.8 86,706 + Pf1N1B4_83 0.63 -1.6 86,706 + Pf1N1B4_83 0.63 +1.9 86,706 + Pf1N1B4_83 0.63 +1.0 86,707 - Pf1N1B4_83 0.63 -1.7 86,707 - Pf1N1B4_83 0.63 -2.1 86,707 - Pf1N1B4_83 0.63 -0.1 86,753 - Pf1N1B4_83 0.69 +0.9 86,774 - Pf1N1B4_83 0.72 -0.7 87,042 + +0.9 87,042 + -1.3 87,149 + -2.1 87,149 + +0.6 87,149 + -0.0 87,149 + -1.5 87,149 + -1.0 87,149 + -3.1 87,149 + -0.3 87,149 + -2.0 87,149 + +1.1 87,149 + -2.1 87,149 + +0.6 87,149 + -0.3 87,149 + -1.3 87,149 + +1.2 87,149 + -0.3 87,149 + -0.3 87,149 + +2.4 87,149 + -1.8 87,150 - +0.1 87,150 - +0.2 87,150 - -0.2 87,150 - +0.1 87,150 - -2.0 87,150 - -1.8 87,150 - -0.0 87,150 - -0.3 87,150 - -0.7 87,150 - +0.9 87,150 - -1.1 87,150 - -0.9 87,150 - +0.9 87,370 + Pf1N1B4_84 0.12 +0.3 87,370 + Pf1N1B4_84 0.12 -1.3 87,370 + Pf1N1B4_84 0.12 +0.1 87,370 + Pf1N1B4_84 0.12 -1.8 87,370 + Pf1N1B4_84 0.12 +1.0 87,370 + Pf1N1B4_84 0.12 -2.6 87,371 - -1.5 87,415 + Pf1N1B4_84 0.16 -0.8 87,473 + Pf1N1B4_84 0.21 +0.3 87,474 - -1.2 87,474 - Pf1N1B4_84 0.21 +0.9 87,474 - Pf1N1B4_84 0.21 +0.3 87,474 - Pf1N1B4_84 0.21 -2.4 87,651 + -0.9 87,712 + Pf1N1B4_84 0.42 -0.2 87,712 + Pf1N1B4_84 0.42 -2.6 87,713 - Pf1N1B4_84 0.42 +0.8 87,713 - Pf1N1B4_84 0.42 +0.7 87,713 - Pf1N1B4_84 0.42 -1.6 87,713 - Pf1N1B4_84 0.42 +2.3 87,769 + Pf1N1B4_84 0.46 -0.1 87,770 - Pf1N1B4_84 0.47 +0.9 87,806 - Pf1N1B4_84 0.50 +0.0 87,806 - Pf1N1B4_84 0.50 +2.8 87,806 - Pf1N1B4_84 0.50 -0.9 87,856 + Pf1N1B4_84 0.54 -0.0 87,856 + Pf1N1B4_84 0.54 +1.9 87,890 + Pf1N1B4_84 0.57 -0.9 87,890 + Pf1N1B4_84 0.57 +0.3 87,891 - Pf1N1B4_84 0.57 -3.7 87,925 + Pf1N1B4_84 0.60 +1.5 88,168 + Pf1N1B4_84 0.81 -0.5 88,168 + Pf1N1B4_84 0.81 -1.1 88,169 - Pf1N1B4_84 0.81 +1.0 88,169 - Pf1N1B4_84 0.81 +1.9 88,173 + Pf1N1B4_84 0.82 -3.1 88,195 + -1.2 88,195 + Pf1N1B4_84 0.84 -3.2 88,195 + Pf1N1B4_84 0.84 -1.2 88,195 + -0.9 88,196 - Pf1N1B4_84 0.84 -1.2 88,196 - Pf1N1B4_84 0.84 -0.0 88,196 - Pf1N1B4_84 0.84 -1.7 88,197 + Pf1N1B4_84 0.84 -1.1 88,198 - Pf1N1B4_84 0.84 -1.9 88,345 + +2.1 88,345 + +1.9 88,345 + -0.7 88,346 - -1.5 88,346 - -2.1 88,346 - +3.1 88,347 + +0.6 88,363 + +2.1 88,363 + -0.7 88,363 + -1.9 88,363 + -1.6 88,364 - +0.7 88,381 + +0.4 88,382 - -2.6 88,422 - +0.9 88,485 + -1.9 88,500 - +0.5 88,519 + -2.3 88,520 - -1.9 88,617 + Pf1N1B4_85 0.13 +0.2 88,617 + Pf1N1B4_85 0.13 -1.2 88,667 + Pf1N1B4_85 0.19 +0.6 88,668 - Pf1N1B4_85 0.19 -1.1 88,754 + Pf1N1B4_85 0.29 -1.0 88,754 + Pf1N1B4_85 0.29 -1.6 88,755 - Pf1N1B4_85 0.29 -3.1 88,820 + Pf1N1B4_85 0.37 +1.3 88,820 + Pf1N1B4_85 0.37 -0.2 88,994 + Pf1N1B4_85 0.58 +0.9 88,994 + Pf1N1B4_85 0.58 +0.5 88,995 - Pf1N1B4_85 0.59 +0.5 88,996 + +0.0 89,070 - Pf1N1B4_85 0.68 -0.9 89,070 - Pf1N1B4_85 0.68 -2.6 89,070 - Pf1N1B4_85 0.68 -0.2 89,311 + -1.5 89,311 + -0.4 89,312 - +1.3 89,312 - +0.4 89,312 - -0.4 89,312 - -1.7
Or see this region's nucleotide sequence