Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4425 and Pf1N1B4_4426 overlap by 4 nucleotides Pf1N1B4_4426 and Pf1N1B4_4427 overlap by 11 nucleotides Pf1N1B4_4427 and Pf1N1B4_4428 overlap by 14 nucleotides Pf1N1B4_4428 and Pf1N1B4_4429 are separated by 17 nucleotides
Pf1N1B4_4425 - Aspartate aminotransferase (EC 2.6.1.1), at 4,885,764 to 4,886,975
_4425
Pf1N1B4_4426 - Glutamate transport membrane-spanning protein, at 4,886,972 to 4,887,706
_4426
Pf1N1B4_4427 - Glutamate transport membrane-spanning protein, at 4,887,696 to 4,888,484
_4427
Pf1N1B4_4428 - ABC-type polar amino acid transport system, ATPase component, at 4,888,471 to 4,889,307
_4428
Pf1N1B4_4429 - No significant database matches, at 4,889,325 to 4,889,921
_4429
Position (kb) 4887
4888
4889 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4886.744 kb on - strand, within Pf1N1B4_4425 at 4886.748 kb on - strand, within Pf1N1B4_4425 at 4887.026 kb on + strand at 4887.558 kb on - strand, within Pf1N1B4_4426 at 4888.017 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.029 kb on + strand, within Pf1N1B4_4427 at 4888.030 kb on - strand, within Pf1N1B4_4427 at 4888.065 kb on + strand, within Pf1N1B4_4427 at 4888.065 kb on + strand, within Pf1N1B4_4427 at 4888.391 kb on + strand, within Pf1N1B4_4427 at 4888.395 kb on + strand, within Pf1N1B4_4427 at 4888.395 kb on + strand, within Pf1N1B4_4427 at 4888.763 kb on - strand, within Pf1N1B4_4428 at 4889.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 4,886,744 - Pf1N1B4_4425 0.81 -1.1 4,886,748 - Pf1N1B4_4425 0.81 -1.7 4,887,026 + -0.9 4,887,558 - Pf1N1B4_4426 0.80 -0.9 4,888,017 + Pf1N1B4_4427 0.41 +0.5 4,888,029 + Pf1N1B4_4427 0.42 -1.7 4,888,029 + Pf1N1B4_4427 0.42 -0.4 4,888,029 + Pf1N1B4_4427 0.42 -2.6 4,888,029 + Pf1N1B4_4427 0.42 -2.7 4,888,029 + Pf1N1B4_4427 0.42 -2.2 4,888,030 - Pf1N1B4_4427 0.42 -0.7 4,888,065 + Pf1N1B4_4427 0.47 -1.6 4,888,065 + Pf1N1B4_4427 0.47 -2.8 4,888,391 + Pf1N1B4_4427 0.88 +0.5 4,888,395 + Pf1N1B4_4427 0.89 +0.1 4,888,395 + Pf1N1B4_4427 0.89 -2.3 4,888,763 - Pf1N1B4_4428 0.35 -1.1 4,889,305 + -1.4
Or see this region's nucleotide sequence