Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2738 and Pf1N1B4_2739 are separated by 90 nucleotides Pf1N1B4_2739 and Pf1N1B4_2740 are separated by 105 nucleotides Pf1N1B4_2740 and Pf1N1B4_2741 overlap by 4 nucleotides
Pf1N1B4_2738 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 3,077,255 to 3,078,175
_2738
Pf1N1B4_2739 - acetyltransferase, GNAT family, at 3,078,266 to 3,078,835
_2739
Pf1N1B4_2740 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 3,078,941 to 3,079,810
_2740
Pf1N1B4_2741 - Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family, at 3,079,807 to 3,080,400
_2741
Position (kb)
3078
3079 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3077.487 kb on + strand, within Pf1N1B4_2738 at 3077.487 kb on + strand, within Pf1N1B4_2738 at 3077.487 kb on + strand, within Pf1N1B4_2738 at 3077.488 kb on - strand, within Pf1N1B4_2738 at 3077.488 kb on - strand, within Pf1N1B4_2738 at 3077.509 kb on - strand, within Pf1N1B4_2738 at 3077.523 kb on + strand, within Pf1N1B4_2738 at 3077.523 kb on + strand, within Pf1N1B4_2738 at 3077.523 kb on + strand, within Pf1N1B4_2738 at 3077.524 kb on - strand, within Pf1N1B4_2738 at 3077.524 kb on - strand, within Pf1N1B4_2738 at 3077.524 kb on - strand, within Pf1N1B4_2738 at 3077.553 kb on + strand, within Pf1N1B4_2738 at 3077.553 kb on + strand, within Pf1N1B4_2738 at 3077.554 kb on - strand, within Pf1N1B4_2738 at 3077.751 kb on + strand, within Pf1N1B4_2738 at 3077.751 kb on + strand, within Pf1N1B4_2738 at 3077.805 kb on + strand, within Pf1N1B4_2738 at 3077.805 kb on + strand, within Pf1N1B4_2738 at 3077.805 kb on + strand, within Pf1N1B4_2738 at 3077.806 kb on - strand, within Pf1N1B4_2738 at 3077.806 kb on - strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.987 kb on - strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.989 kb on - strand, within Pf1N1B4_2738 at 3077.989 kb on - strand, within Pf1N1B4_2738 at 3078.144 kb on + strand at 3078.145 kb on - strand at 3078.145 kb on - strand at 3078.156 kb on + strand at 3078.156 kb on + strand at 3078.157 kb on - strand at 3078.157 kb on - strand at 3078.157 kb on - strand at 3078.184 kb on + strand at 3078.184 kb on + strand at 3078.184 kb on + strand at 3078.184 kb on + strand at 3078.185 kb on - strand at 3078.185 kb on - strand at 3078.185 kb on - strand at 3078.186 kb on + strand at 3078.186 kb on + strand at 3078.186 kb on + strand at 3078.200 kb on + strand at 3078.200 kb on + strand at 3078.200 kb on + strand at 3078.220 kb on + strand at 3078.220 kb on + strand at 3078.221 kb on - strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.232 kb on - strand at 3078.232 kb on - strand at 3078.232 kb on - strand at 3078.232 kb on - strand at 3078.538 kb on + strand, within Pf1N1B4_2739 at 3078.539 kb on - strand, within Pf1N1B4_2739 at 3078.593 kb on + strand, within Pf1N1B4_2739 at 3078.791 kb on + strand at 3078.792 kb on - strand at 3078.792 kb on - strand at 3078.833 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.879 kb on + strand at 3079.057 kb on - strand, within Pf1N1B4_2740 at 3079.127 kb on + strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.166 kb on + strand, within Pf1N1B4_2740 at 3079.268 kb on + strand, within Pf1N1B4_2740 at 3079.311 kb on + strand, within Pf1N1B4_2740 at 3079.312 kb on - strand, within Pf1N1B4_2740 at 3079.312 kb on - strand, within Pf1N1B4_2740 at 3079.312 kb on - strand, within Pf1N1B4_2740 at 3079.445 kb on + strand, within Pf1N1B4_2740 at 3079.445 kb on + strand, within Pf1N1B4_2740 at 3079.446 kb on - strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.680 kb on - strand, within Pf1N1B4_2740
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 3,077,487 + Pf1N1B4_2738 0.25 -0.4 3,077,487 + Pf1N1B4_2738 0.25 +0.6 3,077,487 + Pf1N1B4_2738 0.25 +0.8 3,077,488 - Pf1N1B4_2738 0.25 +0.4 3,077,488 - Pf1N1B4_2738 0.25 -0.1 3,077,509 - Pf1N1B4_2738 0.28 -1.6 3,077,523 + Pf1N1B4_2738 0.29 -2.8 3,077,523 + Pf1N1B4_2738 0.29 -1.2 3,077,523 + Pf1N1B4_2738 0.29 +0.7 3,077,524 - Pf1N1B4_2738 0.29 +1.5 3,077,524 - Pf1N1B4_2738 0.29 -2.2 3,077,524 - Pf1N1B4_2738 0.29 -1.0 3,077,553 + Pf1N1B4_2738 0.32 +2.9 3,077,553 + Pf1N1B4_2738 0.32 -2.4 3,077,554 - Pf1N1B4_2738 0.32 +0.2 3,077,751 + Pf1N1B4_2738 0.54 -0.1 3,077,751 + Pf1N1B4_2738 0.54 +0.8 3,077,805 + Pf1N1B4_2738 0.60 +0.9 3,077,805 + Pf1N1B4_2738 0.60 +0.9 3,077,805 + Pf1N1B4_2738 0.60 +0.3 3,077,806 - Pf1N1B4_2738 0.60 -1.5 3,077,806 - Pf1N1B4_2738 0.60 +1.2 3,077,976 + Pf1N1B4_2738 0.78 +0.3 3,077,976 + Pf1N1B4_2738 0.78 -0.9 3,077,976 + Pf1N1B4_2738 0.78 -1.0 3,077,976 + Pf1N1B4_2738 0.78 +0.8 3,077,976 + Pf1N1B4_2738 0.78 -0.9 3,077,977 - Pf1N1B4_2738 0.78 -0.3 3,077,977 - Pf1N1B4_2738 0.78 -0.4 3,077,977 - Pf1N1B4_2738 0.78 +0.2 3,077,977 - Pf1N1B4_2738 0.78 +2.0 3,077,987 - Pf1N1B4_2738 0.79 +0.1 3,077,988 + Pf1N1B4_2738 0.80 -2.8 3,077,988 + Pf1N1B4_2738 0.80 -0.6 3,077,988 + Pf1N1B4_2738 0.80 -2.9 3,077,988 + Pf1N1B4_2738 0.80 -0.8 3,077,988 + +2.6 3,077,988 + Pf1N1B4_2738 0.80 +1.8 3,077,988 + Pf1N1B4_2738 0.80 -1.7 3,077,988 + Pf1N1B4_2738 0.80 +0.8 3,077,988 + Pf1N1B4_2738 0.80 -1.8 3,077,988 + Pf1N1B4_2738 0.80 -0.9 3,077,988 + Pf1N1B4_2738 0.80 -1.6 3,077,988 + Pf1N1B4_2738 0.80 +0.1 3,077,988 + Pf1N1B4_2738 0.80 +0.1 3,077,988 + Pf1N1B4_2738 0.80 -0.8 3,077,989 - Pf1N1B4_2738 0.80 +1.2 3,077,989 - Pf1N1B4_2738 0.80 -1.1 3,078,144 + -1.8 3,078,145 - -0.5 3,078,145 - +0.4 3,078,156 + +0.3 3,078,156 + -1.9 3,078,157 - +1.4 3,078,157 - +1.3 3,078,157 - +1.0 3,078,184 + -0.8 3,078,184 + +1.2 3,078,184 + -1.3 3,078,184 + +0.4 3,078,185 - +0.2 3,078,185 - -0.9 3,078,185 - -1.9 3,078,186 + -0.5 3,078,186 + -0.9 3,078,186 + +1.0 3,078,200 + +0.7 3,078,200 + -0.9 3,078,200 + -0.1 3,078,220 + -0.8 3,078,220 + +1.0 3,078,221 - +2.9 3,078,231 + -0.2 3,078,231 + +0.5 3,078,231 + +2.0 3,078,231 + -1.1 3,078,231 + +0.6 3,078,232 - -2.5 3,078,232 - -0.8 3,078,232 - -2.0 3,078,232 - +0.4 3,078,538 + Pf1N1B4_2739 0.48 -2.4 3,078,539 - Pf1N1B4_2739 0.48 -3.0 3,078,593 + Pf1N1B4_2739 0.57 -3.7 3,078,791 + +1.1 3,078,792 - -1.3 3,078,792 - -2.1 3,078,833 + -0.6 3,078,851 + -0.2 3,078,851 + +0.1 3,078,851 + -0.9 3,078,851 + -1.1 3,078,852 - +1.1 3,078,852 - -0.8 3,078,852 - -2.3 3,078,852 - -2.4 3,078,852 - +0.8 3,078,879 + -0.8 3,079,057 - Pf1N1B4_2740 0.13 +0.1 3,079,127 + Pf1N1B4_2740 0.21 +0.7 3,079,128 - Pf1N1B4_2740 0.21 +2.3 3,079,128 - Pf1N1B4_2740 0.21 +1.5 3,079,166 + Pf1N1B4_2740 0.26 -0.6 3,079,268 + Pf1N1B4_2740 0.38 -0.1 3,079,311 + Pf1N1B4_2740 0.43 -1.5 3,079,312 - Pf1N1B4_2740 0.43 -3.2 3,079,312 - Pf1N1B4_2740 0.43 +0.2 3,079,312 - Pf1N1B4_2740 0.43 +0.0 3,079,445 + Pf1N1B4_2740 0.58 +0.7 3,079,445 + Pf1N1B4_2740 0.58 -0.3 3,079,446 - Pf1N1B4_2740 0.58 +0.1 3,079,475 + Pf1N1B4_2740 0.61 +1.5 3,079,475 + Pf1N1B4_2740 0.61 -1.3 3,079,475 + Pf1N1B4_2740 0.61 +0.1 3,079,475 + Pf1N1B4_2740 0.61 +1.6 3,079,475 + Pf1N1B4_2740 0.61 +1.1 3,079,476 - Pf1N1B4_2740 0.61 +0.9 3,079,476 - Pf1N1B4_2740 0.61 +0.8 3,079,476 - Pf1N1B4_2740 0.61 -0.1 3,079,476 - Pf1N1B4_2740 0.61 +1.2 3,079,476 - Pf1N1B4_2740 0.61 +0.5 3,079,529 + Pf1N1B4_2740 0.68 -2.5 3,079,529 + Pf1N1B4_2740 0.68 -0.2 3,079,529 + Pf1N1B4_2740 0.68 +1.2 3,079,529 + Pf1N1B4_2740 0.68 +1.4 3,079,529 + Pf1N1B4_2740 0.68 +0.9 3,079,529 + Pf1N1B4_2740 0.68 -2.2 3,079,529 + Pf1N1B4_2740 0.68 -2.0 3,079,529 + Pf1N1B4_2740 0.68 -1.4 3,079,529 + Pf1N1B4_2740 0.68 -2.1 3,079,529 + Pf1N1B4_2740 0.68 -2.0 3,079,530 - Pf1N1B4_2740 0.68 -1.4 3,079,530 - Pf1N1B4_2740 0.68 -0.0 3,079,530 - Pf1N1B4_2740 0.68 +0.0 3,079,530 - Pf1N1B4_2740 0.68 +1.0 3,079,530 - Pf1N1B4_2740 0.68 +1.5 3,079,680 - Pf1N1B4_2740 0.85 +2.2
Or see this region's nucleotide sequence