Experiment: carbon source Sucrose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4329 and Pf1N1B4_4330 are separated by 21 nucleotides Pf1N1B4_4330 and Pf1N1B4_4331 are separated by 28 nucleotides Pf1N1B4_4331 and Pf1N1B4_4332 are separated by 188 nucleotides Pf1N1B4_4332 and Pf1N1B4_4333 are separated by 50 nucleotides
Pf1N1B4_4329 - Acyl dehydratase, at 4,790,204 to 4,790,689
_4329
Pf1N1B4_4330 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 4,790,711 to 4,791,553
_4330
Pf1N1B4_4331 - Transporter, LysE family, at 4,791,582 to 4,792,199
_4331
Pf1N1B4_4332 - Transcriptional regulator, AsnC family, at 4,792,388 to 4,792,840
_4332
Pf1N1B4_4333 - hypothetical protein, at 4,792,891 to 4,793,112
_4333
Position (kb) 4791
4792
4793 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4790.605 kb on + strand, within Pf1N1B4_4329 at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.867 kb on + strand, within Pf1N1B4_4330 at 4790.909 kb on - strand, within Pf1N1B4_4330 at 4791.423 kb on + strand, within Pf1N1B4_4330 at 4791.423 kb on + strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.424 kb on - strand, within Pf1N1B4_4330 at 4791.554 kb on + strand at 4791.575 kb on + strand at 4791.575 kb on + strand at 4791.715 kb on + strand, within Pf1N1B4_4331 at 4791.759 kb on - strand, within Pf1N1B4_4331 at 4791.831 kb on + strand, within Pf1N1B4_4331 at 4791.831 kb on + strand, within Pf1N1B4_4331 at 4791.832 kb on - strand, within Pf1N1B4_4331 at 4792.053 kb on + strand at 4792.147 kb on - strand at 4792.148 kb on + strand at 4792.149 kb on - strand at 4792.149 kb on - strand at 4792.149 kb on - strand at 4792.149 kb on - strand at 4792.199 kb on + strand at 4792.200 kb on - strand at 4792.214 kb on + strand at 4792.214 kb on + strand at 4792.215 kb on - strand at 4792.215 kb on - strand at 4792.240 kb on + strand at 4792.300 kb on - strand at 4792.318 kb on - strand at 4792.348 kb on + strand at 4792.474 kb on + strand, within Pf1N1B4_4332 at 4792.697 kb on + strand, within Pf1N1B4_4332 at 4792.698 kb on - strand, within Pf1N1B4_4332 at 4792.893 kb on - strand at 4792.918 kb on + strand, within Pf1N1B4_4333 at 4792.919 kb on - strand, within Pf1N1B4_4333 at 4792.919 kb on - strand, within Pf1N1B4_4333 at 4792.979 kb on - strand, within Pf1N1B4_4333 at 4792.979 kb on - strand, within Pf1N1B4_4333 at 4793.046 kb on + strand, within Pf1N1B4_4333 at 4793.047 kb on - strand at 4793.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 20mM remove 4,790,605 + Pf1N1B4_4329 0.83 -0.2 4,790,641 + +1.0 4,790,641 + +3.1 4,790,641 + -0.5 4,790,641 + -2.0 4,790,867 + Pf1N1B4_4330 0.19 -0.4 4,790,909 - Pf1N1B4_4330 0.23 -0.6 4,791,423 + Pf1N1B4_4330 0.84 -1.0 4,791,423 + Pf1N1B4_4330 0.84 -0.8 4,791,424 - Pf1N1B4_4330 0.85 +0.1 4,791,424 - Pf1N1B4_4330 0.85 -0.6 4,791,424 - Pf1N1B4_4330 0.85 +0.4 4,791,424 - Pf1N1B4_4330 0.85 +0.7 4,791,424 - +0.2 4,791,424 - Pf1N1B4_4330 0.85 -1.9 4,791,424 - Pf1N1B4_4330 0.85 -0.3 4,791,554 + +0.9 4,791,575 + -0.5 4,791,575 + -1.2 4,791,715 + Pf1N1B4_4331 0.22 -0.5 4,791,759 - Pf1N1B4_4331 0.29 -0.7 4,791,831 + Pf1N1B4_4331 0.40 -3.0 4,791,831 + Pf1N1B4_4331 0.40 -1.8 4,791,832 - Pf1N1B4_4331 0.40 -0.5 4,792,053 + +2.4 4,792,147 - -0.4 4,792,148 + +0.8 4,792,149 - +0.1 4,792,149 - +0.6 4,792,149 - -1.0 4,792,149 - -0.1 4,792,199 + -0.6 4,792,200 - -0.3 4,792,214 + -0.9 4,792,214 + +0.4 4,792,215 - -0.3 4,792,215 - -1.4 4,792,240 + +0.6 4,792,300 - -0.9 4,792,318 - -1.0 4,792,348 + +0.3 4,792,474 + Pf1N1B4_4332 0.19 +0.7 4,792,697 + Pf1N1B4_4332 0.68 -1.2 4,792,698 - Pf1N1B4_4332 0.68 -0.8 4,792,893 - -2.4 4,792,918 + Pf1N1B4_4333 0.12 +1.0 4,792,919 - Pf1N1B4_4333 0.13 -1.7 4,792,919 - Pf1N1B4_4333 0.13 -0.2 4,792,979 - Pf1N1B4_4333 0.40 +0.1 4,792,979 - Pf1N1B4_4333 0.40 +2.0 4,793,046 + Pf1N1B4_4333 0.70 -1.5 4,793,047 - -2.2 4,793,134 - +0.9
Or see this region's nucleotide sequence