Experiment: carbon source Sucrose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4039 and Pf1N1B4_4040 are separated by 72 nucleotides Pf1N1B4_4040 and Pf1N1B4_4041 are separated by 10 nucleotides Pf1N1B4_4041 and Pf1N1B4_4042 are separated by 649 nucleotides
Pf1N1B4_4039 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 4,490,237 to 4,492,021
_4039
Pf1N1B4_4040 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 4,492,094 to 4,492,768
_4040
Pf1N1B4_4041 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 4,492,779 to 4,493,192
_4041
Pf1N1B4_4042 - Isocitrate lyase (EC 4.1.3.1) (from data) , at 4,493,842 to 4,495,167
_4042
Position (kb)
4492
4493
4494 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4491.816 kb on + strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.828 kb on + strand, within Pf1N1B4_4039 at 4491.829 kb on - strand, within Pf1N1B4_4039 at 4491.987 kb on + strand at 4491.988 kb on - strand at 4492.000 kb on - strand at 4492.000 kb on - strand at 4492.025 kb on + strand at 4492.025 kb on + strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.133 kb on - strand at 4492.296 kb on + strand, within Pf1N1B4_4040 at 4492.297 kb on - strand, within Pf1N1B4_4040 at 4492.350 kb on + strand, within Pf1N1B4_4040 at 4492.351 kb on - strand, within Pf1N1B4_4040 at 4492.396 kb on + strand at 4492.563 kb on + strand, within Pf1N1B4_4040 at 4492.585 kb on + strand, within Pf1N1B4_4040 at 4492.586 kb on - strand, within Pf1N1B4_4040 at 4492.761 kb on + strand at 4492.762 kb on - strand at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.879 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.284 kb on + strand at 4493.326 kb on - strand at 4493.329 kb on + strand at 4493.330 kb on - strand at 4493.345 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.425 kb on - strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.551 kb on + strand at 4493.780 kb on + strand at 4493.781 kb on - strand at 4493.781 kb on - strand at 4493.825 kb on + strand at 4493.930 kb on + strand at 4494.111 kb on - strand, within Pf1N1B4_4042
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 20mM remove 4,491,816 + Pf1N1B4_4039 0.88 -1.7 4,491,817 - Pf1N1B4_4039 0.89 -1.7 4,491,817 - Pf1N1B4_4039 0.89 -2.5 4,491,817 - Pf1N1B4_4039 0.89 -1.2 4,491,828 + Pf1N1B4_4039 0.89 -1.8 4,491,829 - Pf1N1B4_4039 0.89 -1.4 4,491,987 + -1.1 4,491,988 - -2.2 4,492,000 - -1.7 4,492,000 - -2.9 4,492,025 + -2.5 4,492,025 + -1.3 4,492,026 - +1.3 4,492,026 - -1.8 4,492,026 - -2.1 4,492,026 - -2.2 4,492,048 - +0.0 4,492,048 - -2.8 4,492,048 - -0.9 4,492,048 - +0.0 4,492,079 - -0.4 4,492,079 - -0.2 4,492,079 - -1.4 4,492,079 - -1.9 4,492,079 - -1.2 4,492,133 - -1.1 4,492,296 + Pf1N1B4_4040 0.30 -0.4 4,492,297 - Pf1N1B4_4040 0.30 -3.2 4,492,350 + Pf1N1B4_4040 0.38 -2.5 4,492,351 - Pf1N1B4_4040 0.38 -2.5 4,492,396 + -0.2 4,492,563 + Pf1N1B4_4040 0.69 -2.1 4,492,585 + Pf1N1B4_4040 0.73 -2.7 4,492,586 - Pf1N1B4_4040 0.73 -2.2 4,492,761 + -4.6 4,492,762 - -1.1 4,492,853 - Pf1N1B4_4041 0.18 -0.5 4,492,853 - Pf1N1B4_4041 0.18 -2.3 4,492,853 - Pf1N1B4_4041 0.18 -2.1 4,492,879 - Pf1N1B4_4041 0.24 -1.2 4,492,994 - Pf1N1B4_4041 0.52 -2.2 4,492,994 - Pf1N1B4_4041 0.52 -1.8 4,493,035 - Pf1N1B4_4041 0.62 +0.9 4,493,035 - Pf1N1B4_4041 0.62 -2.7 4,493,035 - Pf1N1B4_4041 0.62 -2.5 4,493,284 + -1.1 4,493,326 - -1.5 4,493,329 + -1.9 4,493,330 - +0.3 4,493,345 + -1.5 4,493,406 + -2.0 4,493,406 + -0.3 4,493,406 + +0.0 4,493,425 - -2.6 4,493,433 + -2.2 4,493,433 + -1.5 4,493,433 + -2.0 4,493,433 + -2.8 4,493,433 + -1.2 4,493,433 + -1.9 4,493,433 + -0.2 4,493,433 + -0.5 4,493,433 + -1.1 4,493,433 + -3.5 4,493,433 + -1.5 4,493,434 - -0.3 4,493,434 - -0.1 4,493,434 - -0.3 4,493,434 - -1.4 4,493,434 - -0.4 4,493,513 + -2.6 4,493,513 + -1.1 4,493,513 + -2.2 4,493,513 + +0.4 4,493,514 - +1.9 4,493,514 - -2.8 4,493,514 - +0.7 4,493,514 - +0.3 4,493,514 - -1.2 4,493,515 + -2.0 4,493,515 + -1.9 4,493,515 + -0.9 4,493,515 + -2.0 4,493,515 + -1.8 4,493,516 - +0.2 4,493,516 - +0.4 4,493,516 - +1.0 4,493,516 - +0.2 4,493,551 + -1.0 4,493,780 + -1.0 4,493,781 - +0.1 4,493,781 - -0.1 4,493,825 + -0.7 4,493,930 + -0.9 4,494,111 - Pf1N1B4_4042 0.20 +0.2
Or see this region's nucleotide sequence