Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6032 and Pf1N1B4_6033 are separated by 3 nucleotides Pf1N1B4_6033 and Pf1N1B4_6034 overlap by 4 nucleotides Pf1N1B4_6034 and Pf1N1B4_6035 are separated by 56 nucleotides
Pf1N1B4_6032 - Ribose operon repressor, at 495,936 to 496,955
_6032
Pf1N1B4_6033 - D-ribose ABC transporter, permease component RbsC <small>(from data)</small>, at 496,959 to 497,936
_6033
Pf1N1B4_6034 - D-ribose ABC transporter, ATPase component RbsA <small>(from data)</small>, at 497,933 to 499,486
_6034
Pf1N1B4_6035 - D-ribose ABC transporter, substrate-binding component RbsB <small>(from data)</small>, at 499,543 to 500,499
_6035
Position (kb) 497
498
499
500 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 496.936 kb on - strand at 496.990 kb on + strand at 496.994 kb on + strand at 496.995 kb on - strand at 496.995 kb on - strand at 496.995 kb on - strand at 496.995 kb on - strand at 497.290 kb on + strand, within Pf1N1B4_6033 at 497.291 kb on - strand, within Pf1N1B4_6033 at 497.399 kb on + strand, within Pf1N1B4_6033 at 497.400 kb on - strand at 497.480 kb on - strand, within Pf1N1B4_6033 at 497.480 kb on - strand, within Pf1N1B4_6033 at 497.507 kb on - strand, within Pf1N1B4_6033 at 497.878 kb on + strand at 497.879 kb on - strand at 497.879 kb on - strand at 497.879 kb on - strand at 497.879 kb on - strand at 497.907 kb on - strand at 497.930 kb on + strand at 497.931 kb on - strand at 497.931 kb on - strand at 497.931 kb on - strand at 498.165 kb on + strand, within Pf1N1B4_6034 at 498.166 kb on - strand, within Pf1N1B4_6034 at 498.166 kb on - strand, within Pf1N1B4_6034 at 498.170 kb on - strand at 498.227 kb on - strand, within Pf1N1B4_6034 at 498.400 kb on + strand, within Pf1N1B4_6034 at 498.401 kb on - strand, within Pf1N1B4_6034 at 498.401 kb on - strand, within Pf1N1B4_6034 at 498.515 kb on - strand, within Pf1N1B4_6034 at 498.566 kb on - strand, within Pf1N1B4_6034 at 498.590 kb on + strand, within Pf1N1B4_6034 at 498.590 kb on + strand, within Pf1N1B4_6034 at 498.792 kb on + strand, within Pf1N1B4_6034 at 498.792 kb on + strand, within Pf1N1B4_6034 at 498.793 kb on - strand, within Pf1N1B4_6034 at 498.888 kb on + strand at 498.888 kb on + strand, within Pf1N1B4_6034 at 498.888 kb on + strand at 498.889 kb on - strand, within Pf1N1B4_6034 at 498.889 kb on - strand, within Pf1N1B4_6034 at 498.889 kb on - strand, within Pf1N1B4_6034 at 498.889 kb on - strand, within Pf1N1B4_6034 at 499.067 kb on - strand, within Pf1N1B4_6034 at 499.067 kb on - strand, within Pf1N1B4_6034 at 499.067 kb on - strand, within Pf1N1B4_6034 at 499.067 kb on - strand, within Pf1N1B4_6034 at 499.067 kb on - strand, within Pf1N1B4_6034 at 499.342 kb on + strand at 499.342 kb on + strand at 499.342 kb on + strand at 499.431 kb on + strand at 499.431 kb on + strand at 499.431 kb on + strand at 499.431 kb on + strand at 499.486 kb on + strand at 499.544 kb on + strand at 499.544 kb on + strand at 499.544 kb on + strand at 499.545 kb on - strand at 499.545 kb on - strand at 499.545 kb on - strand at 499.777 kb on + strand, within Pf1N1B4_6035 at 499.795 kb on - strand, within Pf1N1B4_6035 at 500.027 kb on + strand, within Pf1N1B4_6035 at 500.027 kb on + strand, within Pf1N1B4_6035 at 500.028 kb on - strand, within Pf1N1B4_6035 at 500.068 kb on + strand, within Pf1N1B4_6035 at 500.069 kb on - strand, within Pf1N1B4_6035 at 500.324 kb on + strand, within Pf1N1B4_6035 at 500.325 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 496,936 - +0.1 496,990 + +1.1 496,994 + -2.0 496,995 - -2.9 496,995 - -0.5 496,995 - +0.9 496,995 - -0.1 497,290 + Pf1N1B4_6033 0.34 -2.6 497,291 - Pf1N1B4_6033 0.34 -0.2 497,399 + Pf1N1B4_6033 0.45 +0.0 497,400 - -1.5 497,480 - Pf1N1B4_6033 0.53 +2.0 497,480 - Pf1N1B4_6033 0.53 +1.2 497,507 - Pf1N1B4_6033 0.56 -1.1 497,878 + -0.1 497,879 - -0.8 497,879 - -1.0 497,879 - -1.7 497,879 - -1.0 497,907 - -1.5 497,930 + +1.2 497,931 - -2.2 497,931 - +0.0 497,931 - +0.1 498,165 + Pf1N1B4_6034 0.15 -1.9 498,166 - Pf1N1B4_6034 0.15 -2.0 498,166 - Pf1N1B4_6034 0.15 -2.7 498,170 - -2.0 498,227 - Pf1N1B4_6034 0.19 -1.7 498,400 + Pf1N1B4_6034 0.30 -1.9 498,401 - Pf1N1B4_6034 0.30 +0.1 498,401 - Pf1N1B4_6034 0.30 -2.4 498,515 - Pf1N1B4_6034 0.37 -1.1 498,566 - Pf1N1B4_6034 0.41 +1.2 498,590 + Pf1N1B4_6034 0.42 -3.3 498,590 + Pf1N1B4_6034 0.42 -1.3 498,792 + Pf1N1B4_6034 0.55 -2.9 498,792 + Pf1N1B4_6034 0.55 -2.7 498,793 - Pf1N1B4_6034 0.55 -2.5 498,888 + +0.5 498,888 + Pf1N1B4_6034 0.61 -2.3 498,888 + +1.0 498,889 - Pf1N1B4_6034 0.62 +0.5 498,889 - Pf1N1B4_6034 0.62 +1.1 498,889 - Pf1N1B4_6034 0.62 +0.5 498,889 - Pf1N1B4_6034 0.62 -2.5 499,067 - Pf1N1B4_6034 0.73 -2.8 499,067 - Pf1N1B4_6034 0.73 -1.3 499,067 - Pf1N1B4_6034 0.73 -2.6 499,067 - Pf1N1B4_6034 0.73 -2.2 499,067 - Pf1N1B4_6034 0.73 +0.8 499,342 + -0.2 499,342 + +0.2 499,342 + +0.5 499,431 + -4.0 499,431 + -1.9 499,431 + -0.4 499,431 + +0.9 499,486 + -2.0 499,544 + -0.0 499,544 + -1.8 499,544 + +0.4 499,545 - -2.7 499,545 - -0.6 499,545 - -1.4 499,777 + Pf1N1B4_6035 0.24 -1.3 499,795 - Pf1N1B4_6035 0.26 +0.7 500,027 + Pf1N1B4_6035 0.51 +0.3 500,027 + Pf1N1B4_6035 0.51 +0.3 500,028 - Pf1N1B4_6035 0.51 +0.2 500,068 + Pf1N1B4_6035 0.55 +1.5 500,069 - Pf1N1B4_6035 0.55 -0.9 500,324 + Pf1N1B4_6035 0.82 +3.3 500,325 - +0.0
Or see this region's nucleotide sequence