Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5896 and Pf1N1B4_5897 overlap by 4 nucleotides Pf1N1B4_5897 and Pf1N1B4_5898 are separated by 173 nucleotides Pf1N1B4_5898 and Pf1N1B4_5899 are separated by 4 nucleotides
Pf1N1B4_5896 - Polysaccharide biosynthesis protein, at 339,330 to 340,634
_5896
Pf1N1B4_5897 - FIG00963512: hypothetical protein, at 340,631 to 342,550
_5897
Pf1N1B4_5898 - hypothetical protein, at 342,724 to 343,332
_5898
Pf1N1B4_5899 - FIG00954746: hypothetical protein, at 343,337 to 344,551
_5899
Position (kb) 340
341
342
343 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 340.202 kb on - strand, within Pf1N1B4_5896 at 340.291 kb on - strand at 340.536 kb on + strand at 340.536 kb on + strand at 340.536 kb on + strand at 340.537 kb on - strand at 340.537 kb on - strand at 340.537 kb on - strand at 340.705 kb on + strand at 340.757 kb on - strand at 340.929 kb on - strand, within Pf1N1B4_5897 at 341.033 kb on + strand, within Pf1N1B4_5897 at 341.033 kb on + strand, within Pf1N1B4_5897 at 341.034 kb on - strand, within Pf1N1B4_5897 at 341.382 kb on - strand, within Pf1N1B4_5897 at 341.382 kb on - strand, within Pf1N1B4_5897 at 341.382 kb on - strand, within Pf1N1B4_5897 at 341.391 kb on - strand, within Pf1N1B4_5897 at 341.456 kb on + strand, within Pf1N1B4_5897 at 341.517 kb on - strand, within Pf1N1B4_5897 at 341.664 kb on - strand, within Pf1N1B4_5897 at 341.696 kb on + strand, within Pf1N1B4_5897 at 341.697 kb on - strand, within Pf1N1B4_5897 at 341.705 kb on + strand, within Pf1N1B4_5897 at 341.748 kb on + strand, within Pf1N1B4_5897 at 342.358 kb on - strand, within Pf1N1B4_5897 at 342.368 kb on - strand at 342.437 kb on + strand at 342.438 kb on - strand at 342.643 kb on - strand at 342.643 kb on - strand at 342.721 kb on + strand at 342.721 kb on + strand at 342.905 kb on - strand, within Pf1N1B4_5898 at 342.919 kb on + strand, within Pf1N1B4_5898 at 342.920 kb on - strand, within Pf1N1B4_5898 at 342.991 kb on + strand at 343.158 kb on + strand, within Pf1N1B4_5898 at 343.158 kb on + strand, within Pf1N1B4_5898 at 343.159 kb on - strand, within Pf1N1B4_5898 at 343.161 kb on - strand, within Pf1N1B4_5898 at 343.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 340,202 - Pf1N1B4_5896 0.67 +0.8 340,291 - -0.8 340,536 + +1.7 340,536 + -0.8 340,536 + -2.0 340,537 - -0.2 340,537 - +0.6 340,537 - -0.2 340,705 + -1.3 340,757 - -3.0 340,929 - Pf1N1B4_5897 0.16 -0.1 341,033 + Pf1N1B4_5897 0.21 +0.7 341,033 + Pf1N1B4_5897 0.21 -2.9 341,034 - Pf1N1B4_5897 0.21 -0.5 341,382 - Pf1N1B4_5897 0.39 +0.5 341,382 - Pf1N1B4_5897 0.39 +1.4 341,382 - Pf1N1B4_5897 0.39 +0.8 341,391 - Pf1N1B4_5897 0.40 -1.9 341,456 + Pf1N1B4_5897 0.43 -0.2 341,517 - Pf1N1B4_5897 0.46 +3.5 341,664 - Pf1N1B4_5897 0.54 +1.3 341,696 + Pf1N1B4_5897 0.55 -2.1 341,697 - Pf1N1B4_5897 0.56 +2.5 341,705 + Pf1N1B4_5897 0.56 +1.7 341,748 + Pf1N1B4_5897 0.58 +1.7 342,358 - Pf1N1B4_5897 0.90 +2.6 342,368 - -0.5 342,437 + -2.4 342,438 - -2.5 342,643 - +1.1 342,643 - +1.3 342,721 + +0.6 342,721 + -2.0 342,905 - Pf1N1B4_5898 0.30 -1.7 342,919 + Pf1N1B4_5898 0.32 +0.5 342,920 - Pf1N1B4_5898 0.32 -3.0 342,991 + +2.8 343,158 + Pf1N1B4_5898 0.71 -0.2 343,158 + Pf1N1B4_5898 0.71 -2.9 343,159 - Pf1N1B4_5898 0.71 -2.4 343,161 - Pf1N1B4_5898 0.72 -1.7 343,430 - +1.8
Or see this region's nucleotide sequence