Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5808 and Pf1N1B4_5809 are separated by 169 nucleotides Pf1N1B4_5809 and Pf1N1B4_5810 are separated by 247 nucleotides Pf1N1B4_5810 and Pf1N1B4_5811 are separated by 67 nucleotides Pf1N1B4_5811 and Pf1N1B4_5812 overlap by 4 nucleotides
Pf1N1B4_5808 - Uricase (urate oxidase) (EC 1.7.3.3), at 241,988 to 242,887
_5808
Pf1N1B4_5809 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 243,057 to 244,004
_5809
Pf1N1B4_5810 - hypothetical protein, at 244,252 to 244,410
_5810
Pf1N1B4_5811 - FIG00958502: hypothetical protein, at 244,478 to 244,792
_5811
Pf1N1B4_5812 - FIG00955248: hypothetical protein, at 244,789 to 245,487
_5812
Position (kb) 243
244
245 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 242.103 kb on + strand, within Pf1N1B4_5808 at 242.104 kb on - strand, within Pf1N1B4_5808 at 242.166 kb on + strand, within Pf1N1B4_5808 at 242.167 kb on - strand, within Pf1N1B4_5808 at 242.216 kb on + strand, within Pf1N1B4_5808 at 242.296 kb on + strand, within Pf1N1B4_5808 at 242.369 kb on + strand at 242.369 kb on + strand, within Pf1N1B4_5808 at 242.370 kb on - strand, within Pf1N1B4_5808 at 242.377 kb on + strand, within Pf1N1B4_5808 at 242.378 kb on - strand, within Pf1N1B4_5808 at 242.378 kb on - strand, within Pf1N1B4_5808 at 242.405 kb on - strand, within Pf1N1B4_5808 at 242.415 kb on + strand, within Pf1N1B4_5808 at 243.411 kb on - strand at 243.454 kb on + strand, within Pf1N1B4_5809 at 243.541 kb on + strand, within Pf1N1B4_5809 at 243.610 kb on + strand, within Pf1N1B4_5809 at 243.610 kb on + strand, within Pf1N1B4_5809 at 243.630 kb on + strand, within Pf1N1B4_5809 at 243.630 kb on + strand, within Pf1N1B4_5809 at 243.630 kb on + strand, within Pf1N1B4_5809 at 243.844 kb on + strand, within Pf1N1B4_5809 at 243.895 kb on + strand, within Pf1N1B4_5809 at 243.898 kb on + strand, within Pf1N1B4_5809 at 243.940 kb on + strand at 243.988 kb on + strand at 244.144 kb on + strand at 244.176 kb on + strand at 244.176 kb on + strand at 244.177 kb on - strand at 244.222 kb on - strand at 244.454 kb on + strand at 244.454 kb on + strand at 244.454 kb on + strand at 244.455 kb on - strand at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.549 kb on - strand at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.733 kb on + strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.805 kb on + strand at 244.805 kb on + strand at 244.806 kb on - strand at 244.806 kb on - strand at 244.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 242,103 + Pf1N1B4_5808 0.13 +0.9 242,104 - Pf1N1B4_5808 0.13 -1.6 242,166 + Pf1N1B4_5808 0.20 +2.1 242,167 - Pf1N1B4_5808 0.20 -0.5 242,216 + Pf1N1B4_5808 0.25 +0.3 242,296 + Pf1N1B4_5808 0.34 +1.5 242,369 + +1.2 242,369 + Pf1N1B4_5808 0.42 -0.8 242,370 - Pf1N1B4_5808 0.42 +0.5 242,377 + Pf1N1B4_5808 0.43 +1.0 242,378 - Pf1N1B4_5808 0.43 +1.8 242,378 - Pf1N1B4_5808 0.43 +1.2 242,405 - Pf1N1B4_5808 0.46 -1.0 242,415 + Pf1N1B4_5808 0.47 -0.4 243,411 - -1.8 243,454 + Pf1N1B4_5809 0.42 +1.1 243,541 + Pf1N1B4_5809 0.51 +0.6 243,610 + Pf1N1B4_5809 0.58 +2.3 243,610 + Pf1N1B4_5809 0.58 +1.2 243,630 + Pf1N1B4_5809 0.60 +2.7 243,630 + Pf1N1B4_5809 0.60 +1.4 243,630 + Pf1N1B4_5809 0.60 +0.3 243,844 + Pf1N1B4_5809 0.83 +0.2 243,895 + Pf1N1B4_5809 0.88 -1.3 243,898 + Pf1N1B4_5809 0.89 +0.9 243,940 + -0.9 243,988 + -1.5 244,144 + -0.4 244,176 + +2.6 244,176 + +0.6 244,177 - +0.2 244,222 - +1.6 244,454 + +1.3 244,454 + -1.1 244,454 + -0.7 244,455 - +0.1 244,548 + Pf1N1B4_5811 0.22 +2.3 244,548 + Pf1N1B4_5811 0.22 -1.9 244,548 + Pf1N1B4_5811 0.22 -0.6 244,548 + Pf1N1B4_5811 0.22 +0.6 244,548 + Pf1N1B4_5811 0.22 +0.6 244,549 - +4.6 244,549 - Pf1N1B4_5811 0.23 -1.9 244,549 - Pf1N1B4_5811 0.23 -3.8 244,549 - Pf1N1B4_5811 0.23 -2.8 244,549 - Pf1N1B4_5811 0.23 -0.3 244,733 + Pf1N1B4_5811 0.81 -2.3 244,734 - Pf1N1B4_5811 0.81 -0.8 244,734 - Pf1N1B4_5811 0.81 +0.4 244,734 - Pf1N1B4_5811 0.81 +1.5 244,734 - Pf1N1B4_5811 0.81 -1.3 244,734 - Pf1N1B4_5811 0.81 -1.9 244,805 + +0.5 244,805 + +1.1 244,806 - -3.3 244,806 - -3.5 244,822 - -0.9
Or see this region's nucleotide sequence