Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5649 and Pf1N1B4_5650 are separated by 175 nucleotides Pf1N1B4_5650 and Pf1N1B4_5651 are separated by 184 nucleotides Pf1N1B4_5651 and Pf1N1B4_5652 are separated by 119 nucleotides
Pf1N1B4_5649 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 76,543 to 77,766
_5649
Pf1N1B4_5650 - Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes, at 77,942 to 79,588
_5650
Pf1N1B4_5651 - ChlD component of cobalt chelatase involved in B12 biosynthesis, at 79,773 to 80,318
_5651
Pf1N1B4_5652 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 80,438 to 81,448
_5652
Position (kb) 77
78
79
80 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 77.320 kb on + strand at 77.320 kb on + strand, within Pf1N1B4_5649 at 77.380 kb on + strand at 77.381 kb on - strand, within Pf1N1B4_5649 at 77.381 kb on - strand, within Pf1N1B4_5649 at 77.622 kb on + strand, within Pf1N1B4_5649 at 77.674 kb on - strand at 77.719 kb on + strand at 77.719 kb on + strand at 77.719 kb on + strand at 77.719 kb on + strand at 77.743 kb on + strand at 77.760 kb on + strand at 77.761 kb on - strand at 77.761 kb on - strand at 77.765 kb on - strand at 77.765 kb on - strand at 77.919 kb on - strand at 78.050 kb on + strand at 78.079 kb on - strand at 78.087 kb on + strand at 78.088 kb on - strand at 78.150 kb on + strand, within Pf1N1B4_5650 at 78.150 kb on + strand, within Pf1N1B4_5650 at 78.657 kb on + strand, within Pf1N1B4_5650 at 78.987 kb on + strand, within Pf1N1B4_5650 at 78.987 kb on + strand, within Pf1N1B4_5650 at 78.987 kb on + strand, within Pf1N1B4_5650 at 78.987 kb on + strand, within Pf1N1B4_5650 at 78.987 kb on + strand, within Pf1N1B4_5650 at 79.393 kb on + strand, within Pf1N1B4_5650 at 79.393 kb on + strand, within Pf1N1B4_5650 at 79.393 kb on + strand, within Pf1N1B4_5650 at 79.394 kb on - strand, within Pf1N1B4_5650 at 79.539 kb on + strand at 79.540 kb on - strand at 79.797 kb on - strand at 80.247 kb on + strand, within Pf1N1B4_5651
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 77,320 + -2.3 77,320 + Pf1N1B4_5649 0.63 +0.2 77,380 + -1.9 77,381 - Pf1N1B4_5649 0.68 -1.7 77,381 - Pf1N1B4_5649 0.68 +2.1 77,622 + Pf1N1B4_5649 0.88 +2.8 77,674 - +0.6 77,719 + +0.4 77,719 + +1.8 77,719 + +1.5 77,719 + -2.1 77,743 + +2.0 77,760 + +0.3 77,761 - +0.8 77,761 - +2.8 77,765 - -1.8 77,765 - +1.2 77,919 - +0.2 78,050 + -2.2 78,079 - +1.3 78,087 + -0.3 78,088 - -1.1 78,150 + Pf1N1B4_5650 0.13 +1.0 78,150 + Pf1N1B4_5650 0.13 +2.4 78,657 + Pf1N1B4_5650 0.43 +0.0 78,987 + Pf1N1B4_5650 0.63 -1.1 78,987 + Pf1N1B4_5650 0.63 +1.5 78,987 + Pf1N1B4_5650 0.63 -2.7 78,987 + Pf1N1B4_5650 0.63 +0.6 78,987 + Pf1N1B4_5650 0.63 +0.9 79,393 + Pf1N1B4_5650 0.88 -0.5 79,393 + Pf1N1B4_5650 0.88 -2.4 79,393 + Pf1N1B4_5650 0.88 -1.1 79,394 - Pf1N1B4_5650 0.88 -2.1 79,539 + -1.7 79,540 - -1.4 79,797 - -1.3 80,247 + Pf1N1B4_5651 0.87 -0.7
Or see this region's nucleotide sequence